Skeletal actin
Skeletal actin mRNA increases in the human heart during ontogenic development and is the major isoform of control and failing adult hearts
... sarcomeric actin mRNA was evaluated as a function of total ...alpha-skeletal actin mRNA represents less than or equal to 20% of sarcomeric actins, it increases to 48 +/- 6% during the first decade ...
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1,25(OH)2 vitamin D3, and retinoic acid antagonize endothelin stimulated hypertrophy of neonatal rat cardiac myocytes
... -skeletal actin CAT, containing 1,400 bp of 5 9 -FS from the mouse a -skeletal actin gene promoter linked to CAT, was kindly provided by ...
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Transcriptional Enhancer Activity of Biochemically Marked Genomic Elements
... “The myosin light chain enhancer and the skeletal actin promoter share a binding site for factors involved in muscle-specific gene expression.” In: Mol Cell Biol.. Essig DA, et al.[r] ...
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Inducible Cre transgenic mouse strain for skeletal muscle-specific gene targeting
... To localize Cre-mediated recombination at the cellular level, we performed histology to detect LacZ expression by assaying β-galactosidase activity following addition of the X-gal substrate. Consistent with the Western ...
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Sarcospan: a small protein with large potential for Duchenne muscular dystrophy
... an autosomal homolog of dystrophin and forms a func- tionally similar utrophin-glycoprotein complex (UGC) where utrophin replaces dystrophin [54-56]. In normal muscle, the UGC is located at the postsynaptic mem- brane of ...
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Distinct roles for Ste20-like kinase SLK in muscle function and regeneration
... levels in muscle tissues and proliferating myoblasts, suggesting a functional role for this kinase in physio- logical processes other than apoptosis [32]. Our previous data showed that SLK is expressed in the muscle mass ...
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The regulation of actin gene expression in "Xenopus" embryos
... The main aim of this work was to identify the important cis-acting regulatory sequences, and the trans-acting factors with which they interact, which are required for the tissue-specific expression of the Xenopus ...
338
Myocardial deletion of Smad4 using a novel α skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract
... The skilled technical assistance of Mrs. Batchelor is gratefully acknowledged. Dr Edna C. Hardeman and Prof. Pierre Chambon are thanked for, respec- tively, gifting the α-skeletal actin promoter and Cre ...
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The Comparative Study of Myofibrillar Proteins of Skeletal Muscles of Some Deep-Sea Fish Species
... The usual depth of the adults of these species range from several hundred meters to upwards of 2,500 m. The tissue composition of these deep-sea fish also suggests a midwater lifestyle. The majority of deep-sea fish ...
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Induction of the skeletal alpha actin gene in alpha 1 adrenoceptor mediated hypertrophy of rat cardiac myocytes
... of skeletal alpha-actin (sACT) mRNA, the fetal/neonatal ...cytoskeletal actin mRNA by 1.8-fold over control, and total alpha-actin mRNA by ...
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Molecular cloning and developmental expression patterns of the MyoD and MEF2 families of muscle transcription factors in the carp
... determination and differentiation of vertebrate skeletal muscle (see Weintraub, 1993). Collectively referred to as myogenic regulatory factors (MRFs), they include the genes for MyoD and myogenin, which are not ...
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Analysis of myofibrillar proteins and transcripts in adult skeletal muscles of the American lobster Homarus americanus: variable expression of myosins, actin and troponins in fast, slow twitch and slow tonic fibres
... Skeletal muscles are characterized using a number of criteria such as contractile properties, ultrastructure, oxidative capacity and distinct myofibrillar isoforms. Crustacean muscle fibers are consistent with ...
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Myosin heavy chain 2A and α Actin expression in human and murine skeletal muscles at feeding; particularly amino acids
... in skeletal muscle tissue should in part resolve this problem, but is only applicable in long-term experiments, since it would be practically difficult to correctly assess quantitative alterations of defined ...
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Distinct Actin and Lipid Binding Sites in Ysc84 Are Required during Early Stages of Yeast Endocytosis
... on actin organization at 30°C was then analysed (Fig ...of actin organization were defined in cells: (1) both actin patches and cables; (2) only patches but these were largely polar- ized; (3) only ...
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Actin myosin and the movements of non-muscle cells
... ii d Myosin extraction 32 e Heavy meromyosin HMM labelling o.f cells 33 f DNAase I treatment of cells 35 Techniques related to Actin and Myosin Isolated from Rabbit Skeletal Muscle 36 a [r] ...
286
Phenotypes induced by NM causing α skeletal muscle actin mutants in fibroblasts, Sol 8 myoblasts and myotubes
... Cell culture and transfection were performed as previ- ously described [12,14]. Briefly, NIH3T3 fibroblasts were transfected with lipofectamin 2000 (Invitrogen), accord- ing to manufacturer's protocol. Coverslips were ...
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Eccentric resistance training and β hydroxy β methylbutyrate free acid affects muscle PGC 1α expression and serum irisin, nesfatin 1 and resistin in rats
... Plausible mechanisms for how exercise can increase irisin concentration have been posited. Researchers have shown that exercise increases PGC-1 α levels in skeletal muscle and increases the muscle-bearing FNDC5 ...
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Human platelets contain gelsolin A regulator of actin filament length
... that actin in human platelets polymerizes in response to various stimuli and that shortening of actin filaments can be regulated by ...to actin in the presence of ...shortens actin filaments ...
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Influence of surface topography on the human epithelial cell response to micropatterned substrates with convex and concave architectures
... Our study supports the notion that the evaluation of spatial distribution in culture of epithelial cells can help in understanding the proliferation and migration potentials of cells grown on the pillar and pit ...
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Robbe_final thesis.docx
... Here, we demonstrate that in Chd4 cardiac null hearts, the atypical expression of skeletal and smooth muscle myofibril isoforms interferes with the proper formation of adjacent sarcomeres into a symmetrical array. ...
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