Soluble epoxide hydrolase activity
Genetic deletion or pharmacological inhibition of soluble epoxide hydrolase reduces brain damage and attenuates neuroinflammation after intracerebral hemorrhage
... Results: ICH induced an increase in sEH protein levels in the hemorrhagic hemisphere from 3 h to 4 days. sEH was expressed in microglia/macrophages, astrocytes, neurons, and endothelial cells in the perihematomal region. ...
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Exported Epoxide Hydrolases Modulate Erythrocyte Vasoactive Lipids during Plasmodium falciparum Infection
... erythrocyte epoxide signaling ...rocyte epoxide signaling lipids, fatty acid preparations were iso- lated from the phospholipid fraction of erythrocytes infected with the PfEH1/2 double-knockout clones ...
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Pharmacological inhibition of soluble epoxide hydrolase ameliorates diet-induced metabolic syndrome in rats
... sEH activity was increased in obese mice, with a large increase during maturation of adipocytes ...increased activity of sEH during obesity and metabolic dysfunction, we hypothesised that increasing EET ...
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Soluble epoxide hydrolase inhibitor enhances synaptic neurotransmission and plasticity in mouse prefrontal cortex
... sEH activity and sEH- EET pathway has been implicated in the disease associated neurotransmission imbalance such as anxiety-related behavior and seizure disorder ...C-terminal epoxide hydrolase (EH) ...
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Basal and inducible anti-inflammatory epoxygenase activity in endothelial cells
... and soluble epoxide hydrolase (sEH) mRNA and ...and epoxide hydro- lase activity: with arachidonic acid (stable epoxide products 5,6-DHET, and 14,15-DHET), linoleic acid ...
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Omega 6 oxylipins generated by soluble epoxide hydrolase are associated with knee osteoarthritis
... Previous studies on knee OA have suggested that the pres- ence of synovitis seen by arthroscopy, MRI, and ultrasound may predict an increased risk of disease progression (2, 28, 29). A role for synovial inflammation in ...
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Original Article Soluble epoxide hydrolase inhibitor, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid, represses human aortic smooth muscle cell proliferation and migration by regulating cell death pathways via the mTOR signaling
... 15], which conversion is the consequence of soluble epoxide hydrolase (sEH) activity [16]. Numerous studies have evoked that inhibition of sEH, with an ensuing decrease in EET degra- dation, ...
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Soluble epoxide hydrolase modulates immune responses in activated astrocytes involving regulation of STAT3 activity
... 35]. Understanding the underlying regulatory mechan- ism may confer a molecular basis for the development disease therapies. Our data show that the expression of sEH was positively associated with the progression of ...
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<p>Soluble epoxide hydrolase inhibition enhances anti-inflammatory and antioxidative processes, modulates microglia polarization, and promotes recovery after ischemic stroke</p>
... by soluble epoxide hydrolase (sEH), thereafter decreasing its anti-in fl ammatory ...enzyme activity and protein stability were linked to lowered risk for ischemic ...
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Genetic deletion of soluble epoxide hydrolase delays the progression of Alzheimer’s disease
... Rabbit anti-sEH (sc-25797), mouse anti-von Willebrand factor (vWF) (sc-365712), goat anti-rabbit IgG FITC- conjugated (sc-2012), Texas red-conjugated (sc-2780), and goat anti-mouse IgG Texas red-conjugated (sc-2781) ...
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Soluble epoxide hydrolase promotes astrocyte survival in retinopathy of prematurity
... Whether the actions of DHA-derived diols can be considered protective or deleterious seems to depend on their local concen- tration, largely determined by the expression and activity of sEH. For example, the ...
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Effects of soluble epoxide hydrolase inhibitor on the expression of fatty acid synthase in peripheral blood mononuclear cell in patients with acute coronary syndrome
... Rae and Graham [14] showed that the C75, which was found to be an inhibitor of FAS [42], effectively blocked pro-atherogenic metabolic responses to a inflammatory factor, preventing this factor from inducing increases in ...
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Involvement of the Saccharomyces cerevisiae Hydrolase Ldh1p in Lipid Homeostasis
... Cells deficient in Ldh1p are characterized by giant LDs accompanied by the accumulation of nonpolar lipids and phos- pholipids. Thus, Ldh1p seems to be required for the mobiliza- tion of LD-stored lipids, which would ...
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A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals
... enzyme activity was defined as the amount of pro- tein capable of releasing 1 μmol pNP, glucose, or reduc- ing sugars from the substrates (releasing 2 μmol glucose from cellobiose) per ...
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Assessment of In Vitro Probiotic Potential of Lactic Acid Bacteria
... Probiotic potential of fermented milks isolates Lactobacillus rhamnosus NS6, Streptococcus thermophilus MD2 and Streptococcus thermophilus MD8 were carried out by performing various in vitro tests. MD2 and MD8 were able ...
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Two different methods for screening of bile salt hydrolase activity in Lactobacillus strains
... salt hydrolase (BSH) activity of intestinal bacteria (including lactobacilli) is one of the indirect ways of decreas- ing a cholesterol level in human ...BSH activity via two methods – thin layer ...
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Functional Characterization of Dipeptide Transport System in Human Jejunum
... The evidence that suggests this includes: a either no or minimal peptide hydrolase activity against glycylglycine or glycylleucine in the intraluminal fluids; b almost unimpaired absorpt[r] ...
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Development of a Multiplexed Activity-Based Protein Profiling Assay to Evaluate Activity of Endocannabinoid Hydrolase Inhibitors
... serine hydrolase activity in native biological ...inhibitor activity and selectivity on a broad range of endocannabinoid hydrolases in mouse brain tissue in a single ...
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Proteomic characterization of serine hydrolase activity and composition in normal urine
... Normal urine (50 mL) was concentrated to 800 μL using centrifugation (4000 g, 30 minutes, 4°C) with a 30 kDa cut-off [Amicon® UFC903024]. The concentrated sample (200 μl) was labeled with 2 μM FP-TAMRA probe [ActivX ...
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Activity Improvement of Organophosphorus Hydrolase Enzyme by Error Prone PCR Method
... We obtained the mutants of oph gene that are able to express OPH enzymes with profound activity; therefore we recommend the error PCR methods as a rapid method for improvement of the protein properties . The epPCR ...
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