Somatic Cell Hybrids
Rescue of Epstein-Barr Virus from Somatic Cell Hybrids of Burkitt Lymphoblastoid Cells
... The D98/HR-1 cells treated with IUDR contained virus particles in the nuclei of about 10%l, of the cells examined and some cells exhibited particles in the cytoplasm; however, no virus p[r] ...
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Host cell-dependent regulation of growth transformation-associated Epstein-Barr virus antigens in somatic cell hybrids.
... 2768 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have analyzed the expression of the three major known growth transformation-associated Epstein-Barr virus EBV pr[r] ...
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Properties of Somatic Cell Hybrids Between Mouse Cells and Simian Virus 40-Transformed Rat Cells
... A positive correlation was found between the loss of human chromosomes and the loss of T antigen from the hybrid cells, suggesting integration of the SV40 genome into the chromosomes of [r] ...
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Physical mapping of genes in somatic cell radiation hybrids by comparative genomic hybridization to cDNA microarrays
... Recent developments in microarray technology have been applied in comparative genomic hybridization (array-CGH) by replacing metaphase chromosomes with arrayed DNAs such as bacterial artificial chromosomes (BACs), P1 ...
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Kinetics of reprogramming in cell fusion hybrids
... differentiated somatic cell nucleus to the totipotent ...reprogram somatic cells (Do and Scholer, ...gram somatic cells, we fused cytoplasts of ES cells (cyESCs) with somatic cells from ...
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Lamin B1 regulates somatic mutations and progression of B cell malignancies
... Figure 4. Nuclear Lamin B1 decreased in B-cell lymphomas. (a) Immunohistochemical tissue microarray analysis of nuclear Lamin B1 in tissue biopsies of B-cell malignancies showing decreasing LMNB1 staining ...
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Chromatin reprogramming during the somatic to reproductive cell fate transition in plants
... The chromatin state at stage 2-II is established gradually and asynchronously among histone modifications. Typically, H1 eviction precedes all other changes sequentially affecting heterochromatin and euchromatin, with ...
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Effects of somatic cell counts in milk on physical and chemical characteristics of yoghurt
... Mastitis is one of the most serious diseases that can affect quality of milk and milk products, it’s derived from it. In the present study, yoghurts were made from cows’ milk with four different somatic ...
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A reinvestigation of somatic hypermethylation at the PTEN CpG island in cancer cell lines
... We designed bisulphite-based PCR assays to determine the methylation status of six regions (designated regions 1-6) within the PTEN CpG island. The regions exam- ined for methylation, the position of these regions rela- ...
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RESEARCHES ON CHEMICAL COMPOSITION AND SOMATIC CELL COUNT IN RAW COW MILK
... Somatic cell count was higher in summer and autumn (over 500,000 cells/ml from June to October) and lower at the end of winter and beginning of spring (lower than 400,000 cells/ml from January till April) ...
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Environmental split between germ cell parasitism and somatic cell synergism in chimeras of a colonial urochordate
... The apparent synergism for fine-tuning a plastic combination of the genetic components in Botryllus chimerical soma (to better fit adverse environmental conditions) may represent a typical form of a green-beard (Dawkins, ...
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Direct control of somatic stem cell proliferation factors by the Drosophila testis stem cell niche
... Fig. 6. Sav in the adult testis. (A) Organisation of the sav locus, the sav-T2A-GFPnls construct, and the sav::GFPnls transcriptional reporters. Blue line, extent of Zfh1 DamID peak (grey shading); blue box, RCSI-like ...
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Brimonidine prevents axonal and somatic degeneration of retinal ganglion cell neurons
... Unilateral IOP elevation in male Sprague-Dawley rats (weight range, 350 - 400 g) was achieved by laser photo- coagulation of episcleral and limbal veins as described previously [11]. Rats were anesthetized with a mixture ...
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CTCF Expression Is Essential for Somatic Cell Viability and Protection against Cancer
... Protein extracts were prepared with cell lysis buffer containing 20 mM Tris-HCl (pH 8), 150 mM NaCl, 1% (v/v) Triton X-100, 0.1% (v/v) SDS, 0.5% (w/v) sodium deoxycholate and EDTA-free protease inhibitor cocktail ...
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Somatic mutations in plasma cell-free DNA are diagnostic markers for esophageal squamous cell carcinoma recurrence
... whether somatic mutations identified in tumor samples could be detected in the matched cfDNA ...Twenty-nine somatic mutations detected in cfDNA samples were concordant with those in the primary or recurrent ...
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HLA Identical Dendritic Leukemic Cell Hybrids Generate Specific CTLs in Vitro
... tumor cell fusion hybrids using solid carcinoma cells, in mouse models or in vitro with human cells such as breast and ovarian carcinoma, has been shown to elicit potent anti tumor effects via the induction ...
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SEPARATION OF LINKED MARKERS IN CHINESE HAMSTER CELL HYBRIDS: MITOTIC RECOMBINATION IS NOT INVOLVED
... The two mechanisms of mitotic recombination and chromosome breakage leading to HPRT+ G6PD- segregants from hybrid 9Y11 may be distinguished by subjecting the nonparental segre[r] ...
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Novel Candidate Genes for Somatic Cell Count in Frizarta Dairy Sheep
... Somatic cell count (SCC) in dairy sheep milk is important in many aspects, including health and production. As in dairy cows, mastitis in dairy ewes is associated with increased SCC in milk [1]. Hence, milk ...
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Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish
... To remove the egg pronucleus, we placed recip- ient eggs in an agar plate filled with Hank’s saline solution. The second polar body of the dechorionated egg was visible under a 403 stereomicroscope. The egg nucleus ...
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Segregation and Recombination of Solanum brevidens Synteny Groups in Progeny of Somatic Hybrids With S. tuberosum: Intragenomic Equals or Exceeds Intergenomic Recombination
... To begin to understand early events in introgression, we have analyzed recombination as deduced from the inheritance of molecular genetic markers in the first few backcros[r] ...
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