Somatic cell nuclear transfer (SCNT)
Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
... bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early development stage ...
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The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer
... of nuclear reprogramming following somatic cell nuclear transfer (SCNT) is thought to depend on factors present in the ...the somatic cell type on the outcome of ...
6
Generation of GHR modified pigs as Laron syndrome models via a dual sgRNAs/Cas9 system and somatic cell nuclear transfer
... fibroblasts; SCNT: somatic cell nuclear transfer; T7ENI: T7 endonuclease I; q‑PCR: quantitative polymerase chain reaction; WB: western blotting; IHC: immunohistochemical; CRISPR/Cas9: ...
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Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish
... Somatic cell nuclear transfer (SCNT) has been performed extensively in fish since the 1960s with a generally low efficiency of approximately ...about somatic nuclear ...
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Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer
... GTKO pigs were generated using traditional homolo- gous recombination (HR), zinc-finger nuclease (ZFN) gene editing technologies and somatic cell nuclear transfer (SCNT) methods [6–10]. ...
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Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer
... Somatic cell nuclear transfer (SCNT) was performed as previously described ...before nuclear transfer and then injected into the perivi- telline space of an enucleated ...
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Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors
... Interspecies somatic cell nuclear transfer (iSCNT) involves the transfer of a nucleus or cell from one species into the cytoplasm of an enucleated oocyte from ...the ...
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The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos
... from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic ...the nuclear ...
9
Generation of bovine transgenics using somatic cell nuclear transfer
... using SCNT to create transgenic ani- mals is that unlike ES cells somatic cells have a definitive ...transgenic cell line with a capacity for 30 PDs [17], Clarke and coworkers have estimated that ...
7
Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)
... programmed cell death induced by c-MYC [45, 46], and, in turn, c-MYC prevents the anti-proliferative function of KLF4 ...in SCNT embryos, and the balance between Klf4 and c-myc was important for the ...
9
Somatic cell nuclear transfer: failures, successes and the challenges ahead
... of SCNT hampers its practical ...wide nuclear remodelling remains a priority in our ...perfect nuclear reprogramming strategy should work across all species, not only ...in nuclear ...
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SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo
... of SCNT embryos from each donor cell were divergent may be related to the status of donor cells such as their passage number and cell cycle phase ...
11
Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology
... To generate MC3R knock-out piglets, biallelically modified colonies and wild-type control colonies were used simultaneously as donor cells for somatic cell nu- clear transfer (SCNT). A total ...
8
Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
... 1000 cell clones. We settled on using a 96-well plate to screen crude cell lysates by ...of cell clones, and will thus likely increase cellular compe- tence for NT ...other cell type use- ful ...
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Development of genome engineering technologies in cattle: from random to specific
... embryo transfer, somatic cell nuclear transfer (SCNT) and cryopreservation, which help us to further our understanding of basic and advanced embryology in animals as well as in ...
9
Generation of Cashmere Goats Carrying an EDAR Gene Mutant Using CRISPR-Cas9-Mediated Genome Editing
... and somatic cell nuclear transfer (SCNT) were used to obtain Cashmere goats with an EDAR gene mutation, providing an animal model and experimental material for studying the relationship ...
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Cloning animals by somatic cell nuclear transfer – biological factors
... of nuclear transfer ...the cell cycle stage of ...with nuclear transfer have uti- lized a serum starvation ...for nuclear transfer and four calves were born from 28 ...
7
Application of new biotechnologies for improvements in swine nutrition and pork production
... embryonic cell nuclear transfer ...called somatic cell nuclear transfer ...the transfer of a nucleus from a donor cell into a mature enucleated oocyte (an ...
16
Gene expression of OCT4, SOX2, KLF4 and MYC (OSKM) induced pluripotent stem cells: identification for potential mechanisms
... by cell fusion, somatic cell nuclear transfer and ectopic expression of defined factors including octamer binding transcription factor 4 (OCT4), SRY related high mobility group box ...
8
Bähr, Andrea (2011): (Re)producing transgenic pigs for xenotransplantation – selection of founder animals and establishment of breeding herds. Dissertation, LMU München: Tierärztliche Fakultät
... The importance of pigs in translational biomedical research has been on a constant increase, as their anatomical and physiological suitability as model animals is distinct (Aigner et al. 2010). Furthermore, pigs are ...
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