splice sites
Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2 dependent introns
... 3' splice sites to identify 3-nt splice variant pairs ...minor splice variant did not exceed the number of these ambiguously mapped ...one splice site corresponding to a RefSeq ...
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The Allele-Specific Suppressor sup-39 Alters Use of Cryptic Splice Sites in Caenorhabditis elegans
... 15/16 splice haps the sup-39 mutation affects the levels of these differ- junction (Figure 4B, lanes 1–5) or for the extrachromo- ent spliced isoforms through changes in the splicing of somal array splice ...
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Activation of cryptic splice sites in murine sarcoma virus-124 mutants.
... In this study we examined the relationship of alterations within the 1,919-base intron between the 5' gag and 3' mos splice sites in MuSV-124 to activation of these sites for Murine sarc[r] ...
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Contributions of Viral Splice Sites andcis-Regulatory Elements to Lentivirus Vector Function
... by splice sites and cis-repressive se- quences (CRS) (or inhibitory sequences [INS]) to allow nu- clear export of Rev-responsive element (RRE)-containing RNAs (3, 7–9, 32, ...the splice donor site ...
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Prediction of donor splice sites using random forest with a new sequence encoding approach
... The purpose of developing the web server is to facilitate easy prediction of donor splice sites by the users working in the area of genome annotations. The developed web server provides flexibility to the ...
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The strength of the HIV 1 3' splice sites affects Rev function
... a splice site however, is exacerbated by the fact that its intrinsic strength can be greatly modified, both positively as well as negatively, by cis-acting splicing regulatory sequences called splicing enhancers ...
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Impairment of alternative splice sites defining a novel gammaretroviral exon within gagmodifies the oncogenic properties of Akv murine leukemia virus
... Finally, we examined if specific regions of the targeted gene/RefSeq have been favored with respect to orientation and position of the integrated provirus. We have recently reported of differences between Akv MLV and an ...
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Intronic sequences and 3' splice sites control Rous sarcoma virus RNA splicing.
... McNALLY AND BEEMON DISCUSSION We demonstrate with heterologous constructs that the control of RSV RNA splicing involves both cis-acting negative regulatory sequences within the intron of[r] ...
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Mutations in the regions of the Rous sarcoma virus 3' splice sites: implications for regulation of alternative splicing.
... The relative amount of spliced src mRNA in pMPM7-transfected cells determined from the ratio of unspliced src to spliced src was increased from 12 to 28%, but the overall level of splice[r] ...
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Method of predicting Splice Sites based on signal interactions
... of splice and splice-like signals in the learning set without a dramatic learning time increase, which substantially improves sensor performance [see Subsection Bayesian sensor ...
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The Research on Identification of Gene Splice Sites by Support Vector Machine
... The recognition of splicing sites is a very important step in the eukaryotic DNA se- quence analysis. Many scholars are working hard to improve the accuracy of identi- fication. Our team carried out research on ...
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Splice junctions in adenovirus 2 early region 4 mRNAs: multiple splice sites produce 18 to 24 RNAs.
... Open reading region 5 is positioned for translation by splicing the leader sequence to the acceptor site at nucleotide 6710 mp 94.7 in molecules of the 0.8-kb mRNA size class Fig.. Howev[r] ...
12
journal.ppat.1006824.pdf
... To identify and catalog regions of the HIV-1 genome that contain critical but unidentified cis-acting RNA elements that impact splicing and other functions, we employed a global syn- onymous mutagenesis strategy. Sixteen ...
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Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1.
... A highly sensitive assay for 5' splice site usage was designed by engineering an ACTl-CUP1 construct with duplicated 5' splice sites; mutations were introduced into[r] ...
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Systematic analysis of splice-site-creating mutations in cancer
... analyze splice-site-creating events that arise from somatic mu- ...on splice-disrupting events in known splice sites, but the current study substantially extends our knowledge into the realm ...
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Improved Primate Foamy Virus Vectors and Packaging Constructs
... heterologous splice sites enabled efficient generation of vec- tor particles while the incorporation of RNA transcribed from the packaging constructs into virus particles could be reduced to barely ...
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Interpretation, Stratification and Validation of Sequence Variants Affecting mRNA Splicing in Complete Human Genome Sequences
... predicted splice sites reside in intronic regions or alternative exons that map far upstream or downstream of constitutively expressed ...these sites are associated with rare, alternatively spliced ...
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Subdivision of Large Introns in Drosophila by Recursive Splicing at Nonexonic Elements
... recursive splice sites/intronic genome (SD ...24.8 sites (see materials and ...recursive splice distances, but we have traced several sites ...nonexo- sites is seven times ...
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Vex seq: high throughput identification of the impact of genetic variation on pre mRNA splicing efficiency
... One of the main goals of personalized medicine is to understand how genetic variations between individuals impact health. Genetic variants can impact health in a number of different ways, one of which is through alter- ...
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The Negative Regulator of Splicing Element of Rous Sarcoma Virus Promotes Polyadenylation
... ⬘ splice sites and a cis-acting negative regulator of splicing (NRS) element within the gag gene, which contribute to the maintenance of unspliced retroviral RNA (2, 13, ...binding sites at its 3 ⬘ ...
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