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Stable gene

Retroviral vectors and transposons for stable gene therapy: advances, current challenges and perspectives

Retroviral vectors and transposons for stable gene therapy: advances, current challenges and perspectives

... fundamental gene is env, which pro- duces proteins embedded in the viral membrane that enable viral attachment to cellular receptors and fusion with target cells, determining the tropism of these viruses ...

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Stable Gene Silencing in Zebrafish with Spatiotemporally Targetable RNA Interference

Stable Gene Silencing in Zebrafish with Spatiotemporally Targetable RNA Interference

... Two platforms are established that can be used either independently or in combination to serve different research needs. The first platform is the in vivo transient RNAi. We demonstrate that this method leads to robust ...

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Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns

Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns

... bridization of macroarrays were carried out as previously described (19). Each RNA sample was independently labeled and hybridized a minimum of three times against swapped filters. Preliminary normalization, logarithmic ...

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Validation of Reference Genes in Cervical Cell Samples from Human Papillomavirus-Infected and -Uninfected Women for Quantitative Reverse Transcription-PCR Assays

Validation of Reference Genes in Cervical Cell Samples from Human Papillomavirus-Infected and -Uninfected Women for Quantitative Reverse Transcription-PCR Assays

... cervical gene expression in studies of cervical HPV ...most stable provides added confidence that, when the focus is on HPV infection, a nor- malization factor derived from GAPDH and RPLP0 is likely to be ...

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EFFECTS OF FLOWER NODE POSITION ON THE MUTABLE V AND STABLE R LOCI IN A CLONE OF NICOTIANA

EFFECTS OF FLOWER NODE POSITION ON THE MUTABLE V AND STABLE R LOCI IN A CLONE OF NICOTIANA

... The somatic effect on a mutable gene system was compared with a stable gene system in the same tissue by counting speckled sectors (changes from us3 to us phenotype) and [r] ...

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Selection of Reference Genes for Expression Studies of Xenobiotic Adaptation in Tetranychus urticae

Selection of Reference Genes for Expression Studies of Xenobiotic Adaptation in Tetranychus urticae

... reference gene identification and selection under diverse experimental ...compared gene expression across several biotic or abiotic variables, such as developmental stage, tissues type, pesticide exposure, ...

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Original Article Molecular cloning and stable knockout strain construction of the AcuE gene in Talaromyces marneffei

Original Article Molecular cloning and stable knockout strain construction of the AcuE gene in Talaromyces marneffei

... (MS)-encoding gene AcuE and to construct stable AcuE gene-knockout strains of Talaromyces ...AcuE gene sequence was identified utilizing a bio- informatics approach, and then it was amplified ...

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Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)

Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)

... one gene compared with each other gene of the set ...indicates stable expression, with the lowest M-value corresponding to the highest stability, and vice ...most stable genes, and his3- 2 was ...

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Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility

Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility

... rice gene LOC_Os01g09010, which we call here ...this gene in all sequenced commelinid monocots; in Brachypodium distachyon (Brachypodium), Setaria viridis (Setaria) and maize there is a one- to-one ortholog ...

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Spatial and temporal localisation of exopolysaccharide gene expression in mucoid and non mucoid Pseudomonas aeruginosa biofilms : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology, Massey Univ

Spatial and temporal localisation of exopolysaccharide gene expression in mucoid and non mucoid Pseudomonas aeruginosa biofilms : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology, Massey University, Manawatu, New Zealand

... To observe the spatial localisation of cells expressing each EPS gene, this strain will be transformed with a plasmid encoding the expression of the stable green fluorescent protein gfp [r] ...

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Stable Transfection of pEGFP-N1-MOG Plasmid to Utilize in Multiple Sclerosis Gene Therapy

Stable Transfection of pEGFP-N1-MOG Plasmid to Utilize in Multiple Sclerosis Gene Therapy

... In order to confirm the entrance of the gene into the des- tination vector, the bacteria were cultured on the plates containing antibiotic. The proliferated colonies entered the colony PCR stage. The proliferated ...

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Genetic regulation of gene expression and splicing during a 10 year period of human aging

Genetic regulation of gene expression and splicing during a 10 year period of human aging

... Fig. 5 Population-level age-specific splicing across the transcriptome. a Manhattan plot of the splicing-age discoveries. Each dot represents a cluster in a gene; the x-axis gives the position of the cluster in ...

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THE UNDERLYING BASES OF GENE EXPRESSION DIFFERENCES IN STABLE TRANSFORMANTS OF THE ROSY LOCUS IN DROSOPHILA MELANOGASTER

THE UNDERLYING BASES OF GENE EXPRESSION DIFFERENCES IN STABLE TRANSFORMANTS OF THE ROSY LOCUS IN DROSOPHILA MELANOGASTER

... That euchromatic po- sition effects may influence transformant gene expression is suggested by the instances of rosy locus transposons that exhibit sex-linked dosage com[r] ...

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Effects of stable ectopic expression of the primary sex determination gene Yob in the mosquito Anopheles gambiae

Effects of stable ectopic expression of the primary sex determination gene Yob in the mosquito Anopheles gambiae

... trans- gene driven by strong promoters, apart from the expected lethal effect on females, may be deleterious or lethal to males, and prevent recovery of transgenic individuals or es- tablishing transgenic ...

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Genetic manipulation and gene expression in the human malaria parasite Plasmodium falciparum

Genetic manipulation and gene expression in the human malaria parasite Plasmodium falciparum

... at gene expression across the whole genome; for example looking at stage-specific mRNA levels using microarrays (Mamoun et al, 2001) or the presence of proteins by mass spectrometry (Florens et al, ...

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Flexible nanopillar-based electrochemical sensors for genetic detection of foodborne pathogens

Flexible nanopillar-based electrochemical sensors for genetic detection of foodborne pathogens

... We demonstrated a reliable process for fabrication of flexible and highly arrayed NPE-based sensing plat- forms by using photo-/soft-lithography and a subsequent electrode patterning process. The NPE showed a great ...

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Agrobacterium-mediated transformation systems of Primula vulgaris

Agrobacterium-mediated transformation systems of Primula vulgaris

... Pre-culture of explants was found crucial in this study, improving explant viability, reducing explant overgrowth from A. tumefaciens and assisting in the identification and removal of bacterial contaminated material ...

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RNAi technology targeting PbGP43 and PbP27 in  Paracoccidioides brasiliensis

RNAi technology targeting PbGP43 and PbP27 in Paracoccidioides brasiliensis

... The RNAi plasmid pCR99 (Figure 1(a)) (provided by Chad Rappleye, Washington University in St. Louis, Mi- ssouri USA) was used to construct the silencing cas- sette targeting P. brasiliensis PbGP43 and PbP27 (Fig- ure ...

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Autoregulated Expression of Schizosaccharomyces pombe Meiosis-Specific Transcription Factor Mei4 and a Genome-Wide Search for Its Target Genes

Autoregulated Expression of Schizosaccharomyces pombe Meiosis-Specific Transcription Factor Mei4 and a Genome-Wide Search for Its Target Genes

... In mei4⌬ cells, the elevated transcript level persisted, scribed when meiosis was induced in wild type, but not in contrast to wild-type cells in which such elevation was in mei4⌬ under the same conditions (Figure 3A). ...

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ON THE NUMBER OF STABLE EQUILIBRIA AND THE SIMULTANEOUS STABILITY OF FIXATION AND POLYMORPHISM IN TWO-LOCUS MODELS

ON THE NUMBER OF STABLE EQUILIBRIA AND THE SIMULTANEOUS STABILITY OF FIXATION AND POLYMORPHISM IN TWO-LOCUS MODELS

... that, for very tight linkage, gene fixation and polymorphism can simultaneously be stable; the polymorphism in this case moves in from a boundary equilibrium as recombination increases[r] ...

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