Streptozotocin Induced Diabetes

Top PDF Streptozotocin Induced Diabetes:

Effect of vitamin D on aortic remodeling in streptozotocin-induced diabetes

Effect of vitamin D on aortic remodeling in streptozotocin-induced diabetes

It is now widely recognised that vitamin D not only plays a major role in bone and calcium metabolism, but may also improve CV health and reduce the risk of CV morbidity and mortality [10,11]. There is evidence that hypovitaminosis D may adversely affect endothelial func- tion [12], leading to increased aortic stiffness [13]. The association between vitamin D deficiency and increased aortic stiffness has been demonstrated in healthy subjects [14,15] and in different chronic inflammatory diseases, including systemic lupus erythematosus [16] and DM [17,18]. Furthermore, the administration of vitamin D to subjects with DM been shown to improve insulin secre- tion and insulin resistance [19]. However, the potential role of vitamin D in diabetic macrovascular complica- tions remains unclear. We designed the current study to investigate the effects of streptozotocin-induced diabetes on the functional/structural properties of the aorta and endothelial function and the potential protective effects of vitamin D supplementation.
Show more

8 Read more

Enhanced plasma ghrelin levels in rats with streptozotocin-induced diabetes

Enhanced plasma ghrelin levels in rats with streptozotocin-induced diabetes

First published online 31 March 2003 Edited by Robert Barouki Abstract Ghrelin is a novel gastrointestinal peptide that stim- ulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such neg- ative energy states as starvation and low body weight. We in- vestigated the dynamics of ghrelin in rats with streptozotocin- induced diabetes, because they present reduced body weight and hyperphagia. The plasma ghrelin levels and gastric preproghre- lin mRNA expression levels of the diabetic rats increased sig- ni¢cantly and their gastric ghrelin levels decreased signi¢cantly.
Show more

5 Read more

EFFECT OF ETHANOLIC EXTRACT OF LEAVES OF PASSIFLORA INSARNATA LINN   IN STREPTOZOTOCIN INDUCED DIABETES IN RATS

EFFECT OF ETHANOLIC EXTRACT OF LEAVES OF PASSIFLORA INSARNATA LINN IN STREPTOZOTOCIN INDUCED DIABETES IN RATS

ABSTRACT: The worldwide prevalence of diabetes mellitus (DM) has risen dramatically over the past two decades; based on current trends, more than 360 million individuals will be having diabetes by year 2030. The present study was designed to investigate the hypoglycemic of effect of ethanolic extract of leaves of Passiflora insarnata linn. in streptozotocin-induced diabetes in rat. The pharmacological investigation suggested that the ethanolic extract of the leaves was beneficial in controlling the blood glucose levels, improves the lipid metabolism and prevents diabetic complications from lipid peroxidation in experimental diabetic rats. From this study, it can be concluded that the ethanolic extract of the plant exhibited protective and ameliorative effects against streptozotocin induced pancreatic cytotoxicity and severe hyperglycaemia by enhancing the peripheral utilization of glucose, correcting the impaired liver glycolysis and limiting gluconeogenic formation and also repairing and rejuvenating the residual beta cell population. These effects may be due to the presence of phenolic compounds, flavonoids, and other phytochemical constituents, which could act synergistically or independently in modulating the activities of glycolytic and gluconeogenic enzymes.
Show more

7 Read more

Serum lipoproteins and apolipoproteins in rats with streptozotocin induced diabetes

Serum lipoproteins and apolipoproteins in rats with streptozotocin induced diabetes

The lipoproteins of rats fed a high sucrose diet and made diabetic by administration of 45 mg/kg of streptozotocin were studied. All lipoprotein classes were found to be present in increased concentrations. The apolipoprotein composition of the various lipoprotein fractions was studied by polyacrylamide-gel electrophoresis in the presence of 8 M urea, isoelectric focusing in the presence of 8 M urea, and sodium dodecyl sulfate gel

9 Read more

Effects of kolaviron on hepatic oxidative stress in streptozotocin induced diabetes

Effects of kolaviron on hepatic oxidative stress in streptozotocin induced diabetes

Apoptosis, a programmed cell death although occurs normally to maintain tissue homeostasis, it can become uncontrolled or dysregulated leading to deleterious patho- logical consequences such as diabetes [24]. Several studies have shown a positive relationship between hyperglycemia- induced oxidative stress and apoptosis [25–28]. Jaeschke et al. also demonstrates that the diabetic state increased oxi- dative stress and cell death of hepatocytes and endothelial cells [29]. Similarly, our work corroborates these observa- tions as apoptosis was significantly increased in the liver post-streptozotocin injection. Treatment with KV however, protected hepatic cells from apoptotic death suggesting the anti-apoptotic property of KV.
Show more

7 Read more

Subhuman Primate Pregnancy Complicated by Streptozotocin Induced Diabetes Mellitus

Subhuman Primate Pregnancy Complicated by Streptozotocin Induced Diabetes Mellitus

Polydipsia, polyuria, polyphagia, and glucosuria followed the administration of streptozotocin to 6 nonpregnant and 15 pregnant monkeys (Macaca mulatta) in the first trimester of pregnancy. The diabetogenic action of the drug was also reflected in an induced but variable deterioration in maternal intravenous glucose tolerance and a marked

12 Read more

Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus

Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus

57. Brooks WW, Bing OHL, Boluyt MO, Malhotra A, Morgan JP, Satoh N, Colucci WS, Conrad CH. Altered inotropic responsiveness and gene expression of hypertrophied myocardium with captopril. Hypertension. 2000;35:1203–9. 58. Prakoso D, De Blasio MJ, Qin C, Rosli S, Kiriazis H, Qian H, Du XJ, Weeks KL, Gregorevic P, McMullen JR, et al. Phosphoinositide 3-kinase (p110α) gene delivery limits diabetes-induced cardiac NADPH oxidase and cardiomyo- pathy in a mouse model with established diastolic dysfunction. Clin Sci.

8 Read more

Streptozotocin-induced diabetes disrupts the body temperature daily rhythm in rats

Streptozotocin-induced diabetes disrupts the body temperature daily rhythm in rats

A combination of melatonin plus insulin restored normal BT and its rhythm, including daily allocation of the acro- phases at night and normal MESOR and amplitudes. These results show a complementary action of insulin and mela- tonin to restore BT, body weight, glycemia and the daily rhythm in long-term diabetic animals. The early treatment of diabetic animals with insulin plus melatonin increased BT above control values and maintained its rhythm. This could be explained by a combined action of melatonin and insulin on BT, since they both act increasing it. When the treatments were interrupted, some of the effects observed during the treatments were lost. Reinstituting treatments restored MESOR, but not amplitude nor acrophases. These results suggest that 15 days of diabetes, even after receiving treatment, lead to persistent alterations of BT rhythm and all rhythmic parameters, along with body weight loss and hyperglycemia, and the reinstitution of the treatments does not result as effective as treating the animals after an early onset. They also suggest a possible interaction between in- sulin and melatonin in diabetic animals that leads to a more efficient treatment of the disruption in the daily rhythm of BT. Since body temperature is a marker rhythm of the state
Show more

13 Read more

A role for glycosphingolipid accumulation in the renal hypertrophy of streptozotocin induced diabetes mellitus

A role for glycosphingolipid accumulation in the renal hypertrophy of streptozotocin induced diabetes mellitus

J Clin Invest. 1993;91(3):797-803. https://doi.org/10.1172/JCI116299. Glucosylceramide (GlcCer) and related glycosphingolipids have been implicated as causal elements in both the growth of cells and in the regulation of hormonal signaling. We therefore studied whether the renal hypertrophy induced by diabetes was associated with enhanced synthesis of glycosphingolipids. 16 d after the induction of diabetes, increases in renal size and concentration of glucocerebroside and ganglioside GM3 were observed paralleling an increase in UDP-Glc concentration. GlcCer synthase and beta-glucosidase- specific activities were no different between control and diabetic kidneys. The apparent Km of the GlcCer synthase with respect to UDP-Glc was 250 microM and was unchanged in the diabetic kidneys. The observed concentrations of UDP-Glc were 149 and 237 microM in control and diabetic kidneys, respectively. The UDP-Glc level is thus rate limiting with regard to GlcCer synthesis. To determine whether the changes in glycolipid content were functionally significant, diabetic and control groups were treated with the GlcCer synthase inhibitor, D-threo-1-phenyl-2-decanoyl-amino-3-morpholino-1- propanol, 2 wk after the induction of diabetes. Kidney weights in the diabetic rats treated with D-threo-1-phenyl-2- decanoylamino-3-morpholino-1-propanol were no different than the control groups.
Show more

8 Read more

Effect of streptozotocin induced diabetes on GLUT 4 phosphorylation in rat adipocytes

Effect of streptozotocin induced diabetes on GLUT 4 phosphorylation in rat adipocytes

Diabetes resulted in 50-60% increase in the particulate PSPase activity and concomitant reductions in cytosolic PSPase activities. Although reduced cytosolic PSPase activity correlated with an inadequate dephosphorylation of LDM GLUT-4, the existence of highly phosphorylated PM GLUT-4 in the presence of increased particulate PSPase activity required additional explanation. To address this problem, we used PM GLUT-4 from diabetic rats as a substrate of particulate PSPase. Highly active diabetic particulate PSPase, which […]

10 Read more

Intestinal Lesions of Streptozotocin-Induced Diabetes and the Effects of Azadirachta Indica Treatment

Intestinal Lesions of Streptozotocin-Induced Diabetes and the Effects of Azadirachta Indica Treatment

indica leaves was most pronounced 1-2 hrs post-dose. In this instance however, the drug was given intravenously to adrenaline-induced hyperglycemic dogs. Proposed mechanisms of hypoglycemic effect of A. indica include inhibition of adrenalin- induced glycogenolysis (13); and an insulinotropic effect (5). In the present study, we observed a modulatory effect of A. indica on feed intake and body weight of diabetic (and non-diabetic) rats exposed to the leaf extract. By 50d, increases in body weight were least in neem-treated rats (Fig. 2). Histological examination of intestinal mucosae of these animals showed intact epithelium (Fig. 4 C); and this suggests that the reduced feed intake and relatively low weight gain in these animals were not a function of compromised nutrient absorption. Rather, a modulatory effect of the drug on leptin production, secretion and/or action, or effects on body adiposity, might be involved. A similar mechanism was reported for exendin-4, an antidiabetic substance from the salivary gland of the Gila monster lizard (14). Future research on A. indica may thus test this hypothesis by quantifying the levels of leptin in the plasma or serum of neem-treated rats.
Show more

10 Read more

Effects of streptozotocin-induced diabetes on the pharmacology of rat conduit and resistance intrapulmonary arteries

Effects of streptozotocin-induced diabetes on the pharmacology of rat conduit and resistance intrapulmonary arteries

Conclusion The results of this study suggest that small resistance pul- monary arteries are relatively resistant to STZ-induced dia- betes. While conduit arteries showed a loss of responsiveness to serotonin and phenyephrine in chronic diabetes, no significant change was seen in resistance arteries. Diabetes had a differential effect on the constric- tor responses to different receptor agonists, with serot- onin affected more than phenylephrine and PGF 2α constriction unchanged, suggesting that there may be dif- ferentially altered expression or function of receptors. The loss of agonist responses may represent a compensatory mechanism to protect the pulmonary circulation from changes in diabetes that promote pulmonary vasocon- striction. In this study, chronic diabetes had no effect on endothelium-dependent or endothelium-independent vasodilation, but an effect on the endothelium cannot be ruled out as a recent study, carried out under different conditions, did find reduced endothelium-dependent vasodilatation in conduit pulmonary arteries.
Show more

10 Read more

Evaluation of antidiabetic efficacy of Murraya koenigii on Streptozotocin induced diabetes in experimental rats

Evaluation of antidiabetic efficacy of Murraya koenigii on Streptozotocin induced diabetes in experimental rats

Diet has been recognized as corner stone in the management of diabetes mellitus. Spices are common dietary adjuncts that contribute to be the taste and flavor of foods. Besides spices it is also known to exert several beneficial physicochemical effects6. Curry leaf tree (Murraya koenigii L., Family: Rutaceae) is a plant which has various important uses in the traditional system of medicine in Eastern Asia. Based on ethnomedicine, Murraya koenigii is used as a stimulant, antidysentric and for the management of diabetes Mellitus. The plant is highly valued for its leaves an important ingredient in an Indian cuisine to promote appetite and digestion. 4
Show more

5 Read more

Original Article Lycopene ameliorates renal function in rats with streptozotocin-induced diabetes

Original Article Lycopene ameliorates renal function in rats with streptozotocin-induced diabetes

Departments of 1 Pathophysiology, 2 Physiology, 3 Biochemistry, School of Basic Medicine, Wannan Medical College, Wuhu 241002, Anhui, PRC Received June 20, 2014; Accepted August 1, 2014; Epub July 15, 2014; Published August 1, 2014 Abstract: Aim: To study the effect of lycopene on ameliorating renal function of diabetic nephropathy. Methods: Sixty male SD rats were divided into four groups: normal untreated (NC-U), normal treatment (NC-L), diabetes untreated (DM-U) and diabetes treatment (DM-L). DM was prepared by a single injection of STZ (70 mg/kg, intraperitoneally) dissolved in 0.1 M citrate buffer (pH 4.5). DM-U and NC-U rats received control diet; DM-L and NC-L rats received lycopene. After treated with lycopene for 8 weeks, blood was obtained for analyzing plasma lipid profiles, glucose and renal function. The kidneys were used to determine SOD activity, malondialdehyde (MDA) level, processed for histological examination and western blot. Results: Treatment of diabetic rats with lycopene decreased the values of blood urea nitrogen, 24 h urea protein and creatinine. The serum lipids like TC, TG, and LDL were decreased and HDL was increased in DM-L rats when compared with those of diabetic rats. Administration of lycopene decreased the levels of MDA content and expression of CTGF, increased Akt/PKB phosphorylation and SOD activity in diabetic renal tissues. Conclusions: Lycopene protects against development of diabetic nephropathy and ameliorates renal function via improving oxidative status and regulating p-Akt and CTGF.
Show more

8 Read more

Role of Angiotensin (1 7) on Renal Hypertrophy  in Streptozotocin Induced Diabetes Mellitus

Role of Angiotensin (1 7) on Renal Hypertrophy in Streptozotocin Induced Diabetes Mellitus

2. Material and Methods 2.1. Animals and Treatment In the present study, we used the well-established STZ-induced diabetes model. STZ destroys the pancreatic beta cells causing a state of insulin dependent diabetes. Male Wistar rats were obtained from the rat colony of the Facultad de Estudios Superiores Iztacala. Animals aged 10 weeks, with initial body weight of 250 ± 20 g were studied.

17 Read more

Chemical and Therapeutic Study of Nannochloropsis oculata on Spleen of Streptozotocin Induced Diabetes in Rats

Chemical and Therapeutic Study of Nannochloropsis oculata on Spleen of Streptozotocin Induced Diabetes in Rats

4 Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt ABSTRACT Diabetes is considered as the most prevalent metabolic disease. Nannochloropsis oculata is well known by its beneficial values due to the presence of various bioactive compounds. In the present study, Streptozotocin (STZ) induced diabetes resulted in a significant (P<0.05) decrease in all hematological measurements. In addition, it caused significant (P<0.05) elevation in the Lipid Peroxidation Product (LPO) level associated with lowering Total Antioxidant Capacity (TAC) level in the spleen tissue. The N. oculata algal extract restored levels of these measurements to normalcy. The present study revealed that N. oculata contains fat soluble vitamin (β-carotene; 79.069 µg/g) and water soluble vitamins (Vitamin B3, B6 and B12; 3.811, 2.531 and 2.475 µg/g, respectively). Furthermore, it contains various micro and macro minerals (Calcium 20.90% and iron 4924 ppm) in addition to presence of phenolics, chlorophylls and carotenoids. The chromatographic analysis identified presence of flavonoids, pyrogallol (179.65 µg/g) and catachine (46.00 µg/g) in ethyl acetate extract. Also, four phenolic compounds (cinnamic acid, p-comaric acid, p-hydroxy benzoic acid and gallic acid) were isolated and identified by column chromatography. In conclusion, Nannochloropsis oculata algal extract showed scavenging activities against oxidative stress induced by STZ in spleen tissue of diabetic rats.
Show more

8 Read more

Effect of Calendula officinalis Extract Against Streptozotocin Induced Diabetes in Male Rats

Effect of Calendula officinalis Extract Against Streptozotocin Induced Diabetes in Male Rats

(F). (H and E x 400) Oral administration of Calendula officinalis extract showed decrease in glucose level and increase in insulin level of both streptozotocin treated groups compared to the positive control group. This might be due to the antioxidants present in Calendula officinalis extract that was confirmed by the phytochemical screening which revealed that it had large amounts of flavonoids and glycosides; moderate amounts of alkaloids Steroids and saponins. These results were in the same line with Khalid et al. [32] who examined the administration of two hundred and six hundred mg/kg of the extract of marigold. The results indicated that there was a significant reduction in blood glucose, while insulin was significantly increased in glucose-loaded rats.
Show more

7 Read more

Protective role of Excoecaria agallocha L. against streptozotocin-induced diabetes and related complications

Protective role of Excoecaria agallocha L. against streptozotocin-induced diabetes and related complications

Hyperglycemia-induced oxidative stress in diabetic control rats indicated by a significant decrease in superoxide dismutase and reduced glutathione level with a significant increase in malondialdehyde (thiobarbituric acid reactive substances) levels was also ameliorated by treatment with extracts. Results and Discussion: Development of neuropathy was evident from marked hyperalgesia (thermal as well as mechanical) and tactile allodynia along with reduced Motor nervous conduction velocity (MNCV). An elevated level of nitrite, tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and interleukin-1β (IL-1β) were also assessed in the sciatic nerve of diabetic neuropathy animals. Treatment with different extracts of E. agallocha L. significantly attenuated elevated thermal and mechanical hyperalgesia. Elevated tactile allodynia assessed using flexible Von Frey filaments was also reversed by extracts. Treatment with both extracts significantly attenuated the parameters of oxidative stress in sciatic nerve of diabetic neuropathy rats. Furthermore, level of nitrite, TNF-α, TGF-β, and IL-1β significantly increased in the sciatic nerve of diabetic neuropathy animals that were ameliorated by treatment with E. agallocha L. extracts. Conclusion:
Show more

13 Read more

Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers

Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers

More detailed studies examining diabetes-induced struc- tural changes in the vagina are currently planned. These studies would quantitatively determine structural changes in the various layers of the vagina, blood vessel density, innervation, etc. Further, since the Western blot assays were performed with whole tissue extracts, the cellular localization of the biochemical markers that were exam- ined in this study remains unclear. Immunohistochemical staining of these markers would provide additional infor- mation pertinent to the effects of diabetic impairment of vaginal function. Future histological studies should also examine changes in structure and protein expression in different regions of the vagina. Only tissue sections from the mid-vagina were used in the current study since this is the region where blood flow was measured. Also, tissues from multiple animals in each treatment group were pooled for Western blot analyses. Values reported as per- cent change (relative to control) were intended to be indicative of general trends and should not be taken as exhaustive quantitative determinations. Finally, vaginal smears were not performed daily to assess potential dis- ruptions in the estrous cycle and the increased number of diabetic rats in diestrus may have been purely coinciden-
Show more

9 Read more

STREPTOZOTOCIN-INDUCED DIABETES INCREASES DISULFIDE BOND. FORMATION ON CARDIAC RYANODINE RECEPTOR (RyR2)

STREPTOZOTOCIN-INDUCED DIABETES INCREASES DISULFIDE BOND. FORMATION ON CARDIAC RYANODINE RECEPTOR (RyR2)

Induction and verification of experimental STZ-induced diabetes. All animal procedures were done in accordance with institutional guidelines established by the Institutional Animal Care and Use Committees. Male Sprague-Dawley rats weighing between 180-190g were purchased from Harlan Laboratories (Indianapolis, IN). After anesthesia (Brevital ® , 25mg/kg i.p.), animals were injected with either a single dose of STZ (50mg/kg) in 0.1M citrate buffer, pH 4.5 or citrate buffer via their tail veins. Three days later, blood glucose levels were measured using a Glucometer II and Glucostix (Peridochrom Glucose GOD-PAP Assay Kit, Roche Molecular Biochemicals,
Show more

54 Read more

Show all 10000 documents...