surface binding
Cell Surface-Binding Motifs of L2 That Facilitate Papillomavirus Infection
... from binding to the cell surface, and BPV1 virions rendered noninfectious by small deletions at either terminus of L2 are still able to bind to the cell surface as for the wild type (38, 40, ...cell ...
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Blumhardt, Philipp Paul (2019): Surface-Integrated Fluorescence Correlation Spectroscopy (SI-FCS) for the quantification of transient membrane and surface binding. Dissertation, LMU München: Fakultät für Physik
... terize surface binding ...of surface densities and still yields accurate results, when the surface concentration of docking sites or fluorescent probes is too high to detect them ...the ...
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Surface Binding of Lysine to Nanoporous PLGA Microparticles
... This work demonstrates the method to control the porosity of nanoporous PLGA microparticles. The nanoporous PLGA microparticles surface was activated with BS3 to form a stable amide bond with primary amine groups. ...
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Structural identifiability of surface binding reactions involving heterogeneous analyte : application to surface plasmon resonance experiments
... involving binding kinetics, greater care has to be taken over the choice of technique, since the assumption that any external input is persistently exciting becomes more restrictive, and computational tractability ...
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Selection and Characterization of Murine Monoclonal Antibodies to Staphylococcus aureus Iron-Regulated Surface Determinant B with Functional Activity In Vitro and In Vivo
... MAb preparation. All animal work was performed in accordance with the Merck Research Laboratories Institutional Animal Care and Use committee guidelines. Two separate immunization protocols were performed in an effort to ...
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Altered interaction of Cis dichlorodiammineplatinum(II) modified alpha 2 macroglobulin (alpha 2M) with the low density lipoprotein receptor related protein/alpha 2M receptor but not the alpha 2M signaling receptor
... determine surface bound ...degradation, surface binding, and uptake were determined by us- ing buffer B in some of the wells containing radioligand and averaged # 10–20% of total binding or ...
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Transfection of a glycosylated phosphatidylinositol anchored folate binding protein complementary DNA provides cells with the ability to survive in low folate medium
... for binding [3H] folate to the surface of transfected 3T3 cells cultured in folate-deficient medium is ...the surface binding capacity of KG cells under similar ...
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Monocyte chemotactic peptide receptor Functional characteristics and ligand induced regulation
... irreversible at the elevated temperatures and exceeded the amount of surface receptor by approximately four-fold, suggesting receptor-mediated peptide endocytosis. Processing of rhodamine-labeled fNLPNTL by ...
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Recent Advances in T Cell Signaling in Aging
... cell surface re- ceptors. Upon binding of antigen to TCR/CD3 complex on the surface of T cells, it induces receptor coupling to transducers such as GTP binding pro- teins or tyrosine kinases, ...
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Maternal celiac disease autoantibodies bind directly to syncytiotrophoblast and inhibit placental tissue transglutaminase activity
... demonstrated binding at the MVM, but the resolution of the technique is insufficient to distinguish tTG at the outer or inner ...outer surface of the pla- ...the surface of the placenta (figures ...
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A Recombinant Clumping Factor A-Containing Vaccine Induces Functional Antibodies to Staphylococcus aureus That Are Not Observed after Natural Exposure
... From the vaccine development standpoint, it is significant that FIG 5 S. aureus is released from immobilized Fg by free ligand or by functional antibodies. (A) Washed PFESA0237 bacteria bound to Fg-coated microplate ...
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Characterization of Caenorhabditis elegans lectin-binding mutants.
... (Because surface lectin binding is restricted to small regions of the adult wild-type animal, mutants missing this lectin binding are difficult to identify in a dissecting micro- sc[r] ...
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Binding of Host Cell Surface Protein Disulfide Isomerase by Anaplasma phagocytophilum Asp14 Enables Pathogen Infection
... cell surface PDI, and Chlamydia trachomatis has been reported to co-opt PDI reductive activity for invasion, but no responsible chlamydial protein has been identi- fied (26, 50, ...
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On the effect of mutations in bovine or camel chymosin on the thermodynamics of binding κ-caseins
... The crystal structure of a chymosin-inhibitor complex (RCSB PDB ID: 1CZI) and previous molecular modelling studies, suggest that κ-casein binds to chymosin in an extended secondary structure.[13, 14] This is supported by ...
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A Single Mutation in the E2 Glycoprotein Important for Neurovirulence Influences Binding of Sindbis Virus to Neuroblastoma Cells
... justed independently and binding was assessed. DMEM ⫹ SB without glucose was supplemented with 11 or 25 mM glucose and 1% FBS. TE bound to N18 cells better than 633 at both concentrations of glucose (Fig. 5C), so ...
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An experimental investigation of metal phthalocyanine germanium interfaces
... It is clear under all bias conditions th a t molecules of this bonding type straddle two dim er rows. However, the observed imago is dram atically different under different bias conditions, giving an indication of the ...
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Computer simulation study of probe-target hybridization in model DNA microarrays: Effect of probe surface density and target concentration
... Investigators have used computer simulations to gain in- sight into the structure and dynamics of DNA at the molecu- lar level, to interpret experimental data, and to test analytical theories. The level of detail used in ...
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Protein surface mimetics: understanding how ruthenium tris(bipyridines) interact with proteins.
... calculated as 4.3, 5.9 and 4.8, respectively. Complex 1 and CCP have relatively similar charges, suggesting they make similar electrostatic interactions with cyt c. Complex 2 has a larger charge indicating increased ...
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Sialic Acid Functions in Enterovirus 70 Binding and Infection
... EV70 binding to HeLa cells decreased in a dose- dependent manner as a result of preincubation of the cells with the broad-specificity, protease-free ...E11 binding to HeLa cells under the same conditions ...
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Neutralization of Human Papillomavirus with Monoclonal Antibodies Reveals Different Mechanisms of Inhibition
... cell surface and extracellular matrix (ECM) of HaCaT ...cell surface-bound capsids. In contrast, MAb U4 prevented binding to the cell surface but not to the ...cell surface but became ...
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