Synechocystis sp.
Cloning and expression of two genes (NHAS2 AND NHAS4) isolated from SYNECHOCYSTIS SP PCC 6803 in yeast
... strain Synechocystis PCC ...from Synechocystis PCC 6803 was PCR amplified with gene-specific primers for the two different Na + /H + antiporter genes (nhaS2 and nhaS4) (Fig ...
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Quantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803
... (measured at the ion gauge) and both precursor and frag- ment ions were detected in the FTICR cell at a resolution of up to 60.000 (m/ Δ m) with a maximum MS/MS rate of about 2 Hz. Instrument mass calibration was better ...
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Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803
... As one core function of cyanobacteria, “Photosyn- thesis” pathway (KO00195) was differentially regulated by hexane exposure at both 24 h and 48 h, in two out of four biological replicates (Additional file 2: Table S2). ...
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Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803
... glucose-tolerant strain of Synechocystis sp. PCC 6803 (21.25 ± 0.55 h and 24.88 ± 0.81 h for GT and ΔapcE mutant, respectively), between 3 to 6 days of culture. These doubling times are similar to that ...
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Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
... Here, we report on our attempts to establish isoprene synthesis in the model cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). In contrast to pre- vious attempts, we used ...
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A Bioelectrochemical Approach to Characterize Extracellular Electron Transfer by Synechocystis sp. PCC6803
... This work reports the use of a simple, mediatorless bioelec- trochemical system to rapidly measure photocurrent produced by Synechocystis sp. PCC6803 without the need to formally grow a mature biofilm on an ...
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Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714
... In this study, the cyanobacterium Synechocystis sp. PCC 6714 has been reported as PHB producer for the first time. The results suggest Synechocystis sp. PCC 6714 to be suitable as a potential ...
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Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803
... cyanobacterial Synechocystis sp. PCC 6803 (hereafter Synechocystis) to butanol was found to be at least 10 times lower than other microbes ever being investigated, including Escherichia coli, Zymo- ...
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Transcriptomic response to prolonged ethanol production in the cyanobacterium Synechocystis sp. PCC6803
... Results: The transcriptome-wide response to continuous ethanol production was examined in Synechocystis sp. PCC6803 using high resolution microarrays. In two independent experiments, ethanol production ...
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Diurnal Regulation of Cellular Processes in the Cyanobacterium Synechocystis sp Strain PCC 6803: Insights from Transcriptomic, Fluxomic, and Physiological Analyses
... of Synechocystis PCC 6803 is tuned to diurnal changes in light ...in Synechocystis PCC 6803 show robust diurnal oscillating behavior, with a majority of the transcripts being upregulated during the early ...
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The alternative sigma factor SigF is a key player in the control of secretion mechanisms in Synechocystis sp. PCC 6803.
... in Synechocystis sp PCC ...on Synechocystis physiology revealed its pleiotropic action and possible targets under SigF control, highlighting its importance for environmental ...the ...
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Overexpression of flv3 improves photosynthesis in the cyanobacterium Synechocystis sp. PCC6803 by enhancement of alternative electron flow
... The flv3 (sll0550) coding region was amplified from Synechocystis sp. PCC6803 genomic DNA by PCR using the primer set 5′-GGAATTATAACCATAATGTTCACT ACCCCCCTCCC-3′ and 5′-GGCATGGAGGACATAT ...
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A study of the impact of mazie SBE I on the [alpha] polyglucan produced in Synechocystis sp strain PCC 6803
... cyanobacterium, Synechocystis, by introducing maize starch branching enzyme I (MSBE I) into a recipient strain, M3, that has only residual levels of branching enzyme activity to produce the double mutant ...in ...
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A bioenergetic assessment of photosynthetic growth of Synechocystis sp. PCC 6803 in continuous cultures
... ver, in our calculation we assumed that PSII/PSI ratio was constant and equal to 1. This ratio is close to the value reported by Fujimori et al. [22], who used Synechocys- tis cultures exposed to a constant light ...
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Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803
... In this study, we have systematically applied different strategies to increase the production of L-lactic acid in Synechocystis. First, we gradually raised the expression level of the L-LDH of L. lactis by gene ...
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The twin arginine translocase pathway : a functional and structural study in Escherichia coli and Synechocystis sp PCC6803
... and Synechocystis, export of TorA-GFP mutants was assessed in Synechocystis ...native Synechocystis Tat substrate, its signal sequence directs GFP to the periplasm (Spence et ...of ...
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A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803
... limitation, so called marker-less strategies have been de- veloped, which allow for the removal of the integrated marker. The first marker-less method was established in Gram-negative bacteria using the nptI-sacB double ...
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Conserved Residues in Ycf54 are required for Protochlorophyllide Formation in Synechocystis sp. PCC 6803.
... HNCI/[eh76"cpf"HNCI/C;I0"Kpfggf."rtgxkqwu"rwnnfqyp"gzrgtkogpvu"*3;+"ujqygf"vjcv"qpn{"HNCI/ [eh76"nqecvgf"ykvjkp"vjg"kpuqnwdng"htcevkqp"ycu"ecrcdng"qh"kpvgtcevkpi"ykvj"E{eK"cpf"cpcn{ugu"q[r] ...
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Functional Expression of Gloeobacter Rhodopsin in Synechocystis sp. PCC6803
... exogenous addition of all‐trans retinal could well increase the amount of functional GR so as to . further increase the extent of the beneficial effect of this retinal‐bas[r] ...
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Ethylene production with engineered Synechocystis sp PCC 6803 strains
... of Synechocystis and ...into Synechocystis by natural transformation for integration of the efe expression cassette and a kanamy- cin resistance cassette at the neutral docking site ...
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