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T-RFLP

Analysing the effect of dietary prebiotics and probiotics on gut bacterial richness and diversity of Asian snakehead fingerlings using T‐RFLP method

Analysing the effect of dietary prebiotics and probiotics on gut bacterial richness and diversity of Asian snakehead fingerlings using T‐RFLP method

... each TRFLP profile for sub- sequent statistical analysis, the T ‐ RFs that were below ...in TRFLP profiles between feeding treat- ments and ...

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Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing

Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing

... The 16S rDNA was amplified from human fecal DNA using the fluorescent-labeled 516f primer (5’-TGCCAG CAGCCGCGGTA-3’; Escherichia coli positions 516 to 532) and 1510r primer (5’-GGTTACCTTGTTACGACTT- 3’; E. coli positions ...

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Chicory (Cichorium intybus L.) and cereals differently affect gut development in broiler chickens and young pigs

Chicory (Cichorium intybus L.) and cereals differently affect gut development in broiler chickens and young pigs

... Dietary fiber, resistant to host-mediated digestion in the small intestine due to lack of endogenous enzymes, impacts many facets of animal health and is associated with gut development especially in young monogastrics. ...

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Resistance and resilience responses of a range of soil eukaryote and bacterial taxa to fungicide application

Resistance and resilience responses of a range of soil eukaryote and bacterial taxa to fungicide application

... The application of plant protection products has the potential to significantly affect soil microbial com- munity structure and function. However, the extent to which soil microbial communities from different trophic ...

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Detection of Streptococcus pneumoniae Strain Cocolonization in the Nasopharynx

Detection of Streptococcus pneumoniae Strain Cocolonization in the Nasopharynx

... plyNCR RFLP has been established as a molecular typing method which correlates well with pulsed-field gel electrophoresis, multilocus sequence typing (MLST), and serotyping for detec- tion of ...of RFLP ...

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Comparison of Self Collected and Physician Collected Vaginal Swabs for Microbiome Analysis

Comparison of Self Collected and Physician Collected Vaginal Swabs for Microbiome Analysis

... rRNA gene sequences assigned to each taxa) is indicated as the percentage of total reads obtained for each sample. The species composition and abundances of microbial communities fell into six groups, which is comparable ...

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Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung

Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung

... 31 T-RFLP analysis includes PCR amplification, using one primer that is fluorescently end-labelled, restric- tion enzyme digestion of the amplicon and detec- tion of the terminal restriction fragment by an ...

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Diversity of bacteria associated with natural aphid populations

Diversity of bacteria associated with natural aphid populations

... This study additionally identified sequence microdiversity from analysis of the length of the 5 ⬘ -TF in the T-RFLP elec- tropherograms of R-type bacteria in A. pisum (Fig. 1K and L) from natural ...

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SNP of cGH gene in Egyptian chicken breeds at MspI site

SNP of cGH gene in Egyptian chicken breeds at MspI site

... Genotyping analysis using PCR-RFLP assay The PCR amplified products of cGH gene of intron 4 for locus GH2 and locus GH3 produced 563 bp fragment length in 84 individuals of four different chicken breeds (Fig. 1a). ...

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Application of DNA typing methods and genetic analysis to epidemiology and taxonomy of Saccharomyces isolates

Application of DNA typing methods and genetic analysis to epidemiology and taxonomy of Saccharomyces isolates

... We have previously described differences in phenotype and virulence among clinical and nonclinical isolates of Saccharomyces. To further characterize these isolates, a comparison of restriction fragment length polymor- ...

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Merozoite surface protein 3 alpha as a genetic marker for epidemiologic studies in Plasmodium vivax: a cautionary note

Merozoite surface protein 3 alpha as a genetic marker for epidemiologic studies in Plasmodium vivax: a cautionary note

... While the ability to distinguish parasite haplotypes using the diverse MSP-3α locus was considered of potential use for certain molecular epidemiology applications, such as tracking recrudescence and infection clonality, ...

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Molecular Analysis of H Antigens Reveals that Human DiarrheagenicEscherichia coli O26 Strains That Carry the eaeGene  Belong to the H11 Clonal Complex

Molecular Analysis of H Antigens Reveals that Human DiarrheagenicEscherichia coli O26 Strains That Carry the eaeGene Belong to the H11 Clonal Complex

... fliC RFLP patterns of E. coli O26 strains. To investigate the fliC RFLP patterns of 57 ...fliC RFLP pat- terns were observed among the 57 strains ...fliC RFLP pattern of three bands of 520, ...

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Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility

Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility

... been previously described (56). This group of genetically re- lated strains is highly prevalent in Southeast Asia, and strains belonging to this grouping did not only show exceptional IS6110 RFLP and spoligotyping ...

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Identification of Nocardia Species by Restriction Endonuclease Analysis of an Amplified Portion of the 16S rRNA Gene

Identification of Nocardia Species by Restriction Endonuclease Analysis of an Amplified Portion of the 16S rRNA Gene

... variant RFLP pattern for Nocardia nova was also ...rRNA RFLP pattern con- sistent with the biochemical identification but was not identifiable by its HSP gene RFLP ...rRNA RFLP patterns which ...

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Pulsed Field Gel Electrophoresis Used To Investigate Genetic Diversity of Haemophilus influenzae Type b Isolates in  Australia Shows Differences between Aboriginal  and Non Aboriginal Isolates

Pulsed Field Gel Electrophoresis Used To Investigate Genetic Diversity of Haemophilus influenzae Type b Isolates in Australia Shows Differences between Aboriginal and Non Aboriginal Isolates

... Analysis of isolates from Aboriginal children living in geo- graphically isolated regions of Australia revealed a relatively small number of genetically distinct RFLP types. A strong association was found between ...

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Comparative Typing of Campylobacter jejuni by Heat Stable Serotyping and PCR Based Restriction Fragment Length Polymorphism Analysis

Comparative Typing of Campylobacter jejuni by Heat Stable Serotyping and PCR Based Restriction Fragment Length Polymorphism Analysis

... the RFLP types of strains within a single Penner serotype were also ob- ...Three RFLP types with identical HhaI and DdeI banding patterns were ...common RFLP type, Hh1Dd1, was identical to genotype ...

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Allelic drop-out may occur with a primer binding site polymorphism for the commonly used RFLP assay for the -1131T>C polymorphism of the Apolipoprotein AV gene

Allelic drop-out may occur with a primer binding site polymorphism for the commonly used RFLP assay for the -1131T>C polymorphism of the Apolipoprotein AV gene

... MseI RFLP assay for -1131T>C on two separate occasions in 82 Asian Indians of known genotype (by sequencing the ...the RFLP in 13 subjects (14%), with three being discordant on both occa- ...on ...

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Male transmission of linear plasmids and mitochondrial DNA in the fungus Neurospora.

Male transmission of linear plasmids and mitochondrial DNA in the fungus Neurospora.

... In crosses showing an RFLP in mtDNA, we have shown that some progeny acquiring male plasmids also contain the male RFLP morph.. In some cases mixtures of male and female m[r] ...

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Predominance of Mycobacterium tuberculosis EAI and Beijing Lineages in Yangon, Myanmar

Predominance of Mycobacterium tuberculosis EAI and Beijing Lineages in Yangon, Myanmar

... Table 1 shows the ST distributions of M. tuberculosis isolates belonging to the two most commonly circulating lineages (Bei- jing and EAI) in Yangon. ST1 (Beijing lineage) was the most prevalent ST among the isolates in ...

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Correlation of wbiI Genotype, Serotype, and Isolate Source within Species of the Burkholderia cepacia Complex

Correlation of wbiI Genotype, Serotype, and Isolate Source within Species of the Burkholderia cepacia Complex

... morphisms were observed experimentally when the wbiI PCR products were digested with BglI or MboII (Fig. 3). There were four different RFLP patterns observed for each restriction FIG. 1. Alignment of J2315 wbiI ...

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