Targeted enrichment and sequencing
Application of a targeted-enrichment methodology for full-genome sequencing of Dengue 1-4, Chikungunya and Zika viruses directly from patient samples.
... a targeted enrichment approach over a Sanger sequencing or an unbiased metagenomic approach, is the number of reads specific to the viral genome are substantially increased and thus allows for ...
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MTTE: an innovative strategy for the evaluation of targeted/ exome enrichment efficiency
... exome enrichment efficiency prior to the sequencing step is of considerable ...exome enrichment, i.e., the Multipoint Test for Targeted-enrichment Efficiency (MTTE), PCR-based approach ...
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Targeted Enrichment for Pathogen Detection and Characterization in Three Felid Species
... and sequencing pathogens from diverse sample sources, including single and pooled nucleic acid extracts, with up to three pathogens per sample originating from experimental (in vitro and in vivo infections) and ...
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Targeted sequencing with enrichment PCR: a novel diagnostic method for the detection of EGFR mutations.
... developed enrichment PCR-UDP method in enhancing the detection of mutations via parallel pyrosequencing with the Roche 454 Junior system (Figure ...PCR enrichment combined with UDP could detect ...
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Clinical utility of targeted gene enrichment and sequencing technique in the diagnosis of adult hereditary spherocytosis
... Sanger sequencing is primarily performed in order to identify the causative mutations in single-gene ...for sequencing is low flux, time-consuming, and ...next-generation sequencing (NGS)-based ...
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Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panel
... and sequencing depth to detect low-abundance, clinically actionable variants in challenging specimens such as ...orthogonal sequencing to improve call accuracy—an approach that we highlight here using ...
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The BisPCR 2 method for targeted bisulfite sequencing
... single sequencing reaction can yield information about multiple target loci for multiple biological ...DNA sequencing library prepara- tion following target enrichment, which can be expensive and ...
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Targeted bisulfite sequencing of the dynamic DNA methylome
... in 80% of each sample with more than 95% probability using Poisson statistics. Since the capture of any CpG in one particular sample is independent of the capture in a different experiment, we simply computed the prob- ...
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Enrichment of sequencing targets from the human genome by solution hybridization
... Conclusions Our results show that the solution hybridization-based method can generate highly uniform coverage of sequence targets that is reproducible across samples. The method has limited, if any, systematic allelic ...
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Targeted next generation sequencing as a tool for precision medicine
... hybridization-based enrichment strategy achieved a me- dian read coverage above 50x for most genes (98 of 100), the results also indicate that the median or mean value alone may not always correctly indicate ...
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Whole genome enrichment and sequencing of Chlamydia trachomatisdirectly from clinical samples
... the targeted genomic DNA to a level that enable sequencing of full genomes (>95-100% coverage of a reference genome) from clinical specimens at >10-fold higher sensitivity than has previously been reported ...
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Metagenomic sequencing with spiked primer enrichment for viral diagnostics and genomic surveillance.
... next-generation sequencing (mNGS), the shotgun sequencing of RNA and DNA from clinical samples, has proved useful for broad-spectrum pathogen detection and the genomic surveillance of viral ...target ...
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Targeted next generation sequencing for molecular diagnosis of Usher syndrome
... developed targeted NGS method based on HaloPlex gene target enrichment technology for the genetic diag- nosis of Usher syndrome provides nearly complete cover- age of all coding regions of the ten USH genes ...
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Whole-genome enrichment and sequencing of Chlamydia trachomatis directly from clinical samples
... the targeted genomic DNA to a level that enable sequencing of full genomes (>95-100% coverage of a reference genome) from clinical specimens at >10-fold higher sensitivity than has previously been reported ...
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Whole-genome enrichment and sequencing of Chlamydia trachomatis directly from clinical samples.
... the targeted genomic DNA to a level that enable sequencing of full genomes (>95-100% coverage of a reference genome) from clinical specimens at >10-fold higher sensitivity than has previously been reported ...
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Targeted. sequencing solutions. Accurate, scalable, fast TARGETED
... Sanger sequencing are not ideal for analyzing large sets of genes, NGS provides the perfect tool to survey hundreds of genes across hundreds of research samples to find rare mutations in a single ...
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Evaluation of next generation sequencing platforms for population targeted sequencing studies
... con ends in the DNA samples after fragmentation prior to library generation. For the ABI SOLiD platform an amplicon end depletion protocol was employed to remove the overrep- resented amplicon ends; this was partially ...
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An advanced enrichment method for rare somatic retroelement insertions sequencing
... current enrichment protocols do not provide enough power for the detection of novel RE inte- grations and thus the sensitivity for somatic RE detec- tion is usually enhanced by increasing the number of ...
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Original Article Mutations in NSCLC identified by a next-generation sequencing targeted sequencing panel
... Next-generation sequencing (NGS) has become a cost-effective approach to screening for a number of genes simultaneously in clinical ...NGS targeted sequencing ...
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Computational optimisation of targeted DNA sequencing for cancer detection.
... The potential of ctDNA for early detection is still to be determined, as it is not yet known how often mutations from the primary tumour can be reliably identified from the analysis of peripheral blood or how this would ...
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