TCR repertoire
Accelerated Loss of TCR Repertoire Diversity in Common Variable Immunodeficiency
... Although the T cell counts were low in all the CVID patients, different pathological mechanisms may be involved between subgroups. A number of intrinsic and extrinsic factors such as stress and infections are known to ...
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Narrowed TCR repertoire and viral escape as a consequence of heterologous immunity
... the repertoire and enriched for predictable TCR ...oligoclonal repertoire with dominant clones with unpredictable TCR ...host’s TCR repertoire and was simulated using ...
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Gene expression pattern of TCR repertoire and alteration expression of IL 17A gene of γδ T cells in patients with acute myocardial infarction
... of TCR repertoire of γδ T cells in AMI patients, analyze the expression levels of regulatory genes Forkhead box P3 (Foxp3) and Interleukin-17A (IL-17A), and characterize the correlation between γδ T cells ...
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Specific T cell activation in an unspecific T cell repertoire
... mature TCR repertoire, as illustrated in Figure 7: the thymic presentation level determines the truncation point of the degeneracy curves, whereas the frequency of presentation determines the sharpness of ...
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Peripheral T cell receptor diversity is associated with clinical outcomes following ipilimumab treatment in metastatic melanoma
... upon the frequency of a particular TCR rearrangement. (Example patient shown in Additional file 1: Figure S1). Two separate metrics were used to further characterize the diversity of the TCR ...
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Review Article Basic research and clinical application of immune repertoire sequencing
... exhibit TCR repertoire alterations [48], with several expansions being found in the CSF or in MS brain lesions [49, ...the TCR repertoire in CSF and blood in 59 patients with relapsing-remit- ...
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Exploring the pre-immune landscape of antigen-specific T cells
... certain TCR nucleotide sequence being produced by the VDJ re- arrangement process can be efficiently recaptured with a probabilistic model that considers V, D, and J gene choices, the number of bases trimmed from ...
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Antiretroviral Therapy Reduces the Magnitude and T Cell Receptor Repertoire Diversity of HIV-Specific T Cell Responses without Changing T Cell Clonotype Dominance
... (BD). TCR repertoire sequencing was carried out as described previously ...a TCR constant region- based primer (5=-TTC ACC CAC CAG CTC AGC TC-3=) and 10 ⫻ uni- versal primer A mix (Clontech, ...
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Improved Assessment of T-Cell Receptor (TCR) VB Repertoire in Clinical Specimens: Combination of TCR-CDR3 Spectratyping with Flow Cytometry-Based TCR VB Frequency Analysis
... each TCR VA or VB family, and the area under the curve for each individual CDR3 peak is ex- pressed as the percentage of the entire CDR3 ...each TCR VA/VB fam- ily; alternatively, the entire TCR VA ...
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Analysis of T cell receptor repertoire of muscle infiltrating T lymphocytes in polymyositis Restricted V alpha/beta rearrangements may indicate antigen driven selection
... alpha/beta TCR repertoire was investigated by RNA-PCR in muscle biopsies from 15 polymyositis patients and 16 controls (6 Duchenne muscular dystrophy and 10 with no inflammatory or dystrophic ...variable ...
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Dominant clonotypes in the repertoire of peripheral CD4+ T cells in rheumatoid arthritis
... clonotypes in the same patient indicated that different mechanisms, and not stimulation by a single arthritogenic antigen, were involved in clonal proliferation. These data support the concept that RA patients have a ...
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Cross reactive public TCR sequences undergo positive selection in the human thymic repertoire
... in TCR repertoire ...stochastic TCR rearrangement versus background genetics on the TCR rep- ertoire, we compared repertoires generated under the same con- ditions from the same progenitor ...
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Characterization of T cell repertoire in patients with graft versus leukemia after donor lymphocyte infusion
... (TCR) repertoire in four patients with re- lapsed chronic myelocytic leukemia who achieved a com- plete remission after infusion of CD4 1 lymphocytes from HLA-identical sibling ...lymphocytes. TCR ...
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High throughput sequencing of CD4+ T cell repertoire reveals disease specific signatures in IgG4 related disease
... The analysis above did not take into consideration the se- quence similarity of different CDR3 clonotypes. Thus, we collected all the CDR3 amino acid clonotypes expanded in at least one individual, evaluated the sequence ...
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IMonitor: A Robust Pipeline for TCR and BCR Repertoire Analysis
... sequencing error also results in artificially increased diversity of the TCR repertoire (Nguyen et al. 2011; Warren et al. 2011). Simply filtering out all low-quality sequences not only removes the sequencing ...
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Immune monitoring and TCR sequencing of CD4 T cells in a long term responsive patient with metastasized pancreatic ductal carcinoma treated with individualized, neoepitope derived multipeptide vaccines: a case report
... we detected two identical and two convergent CDR3 sequences at more than 1 time point, prodding persis- tent, vaccine-specific immunity. Furthermore, formation of convergent sequences against a common target could ...
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Narrowing of Human Influenza A Virus-Specific T Cell Receptor α and β Repertoires with Increasing Age
... gous viral infections the memory pool of CD8 T cells is changed (41–43). With each infection there is a loss of existing memory cells but a retention of cross-reactive memory cells. In the process of T cell attrition, ...
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Simian Immunodeficiency Virus SIVmac239Δnef Vaccination Elicits Different Tat28-35SL8-Specific CD8+ T-Cell Clonotypes Compared to a DNA Prime/Adenovirus Type 5 Boost Regimen in Rhesus Macaques
... It is possible that SIVmac239 ⌬ nef epitope variation affects the protective efficacy of this vaccine, given that coevolution of the host immune system with the diversifying virus may influ- ence the TCR ...
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Antigen-Driven T-Cell Selection in Patients with Cervical Cancer as Evidenced by T-Cell Receptor Analysis and Recognition of Autologous Tumor
... (TCR) repertoire by using measurement of the TCR CDR3 region in PBL, tumor lesions, and TIL from three patients with cervical cancer, from which autologous tumor cell lines could be ...the TCR ...
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Long-term immune reconstitution and T cell repertoire analysis after autologous hematopoietic stem cell transplantation in systemic sclerosis patients
... Clinical follow-up was performed as previously described during the first year after aHSCT [8] and thereafter at yearly intervals plus or minus 6 months. Clinical re- sponse after aHSCT was assessed by the same observer ...
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