The fluorescence-based thermal shift assay
Fluorescence of Senna simea Lam leaf extracts: A possible interference in a fluorescence based assay
... reuptake assay kit. After measuring the intracellular fluorescence, we calculated RFU area below curve reflecting the activity of dopamine transporter (DAT), norepinephrine transporter (NET), serotonin ...
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A fluorescence based assay suitable for quantitative analysis of deadenylase enzyme activity
... deadenylase assay, because the various assays currently available for the biochemical analysis of deadenylase enzymes are time consuming, and less suitable for quantitative analysis and ...assays based on ...
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Development of a Fluorescence Polarization Based Diagnostic Assay for Equine Infectious Anemia Virus
... is based on a region that is immunodominant in lentiviruses and yet is well ...FP assay with larger panels of horse serum in which sensitivity levels of ...
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A fluorescence based sensor assay that monitors general protein aggregation in human cells
... Our sensor is a valuable tool for the development of fluorescence- based screens to identify both genes that affect proteostasis and chemical compounds that lead to protein destabilization. Our stable cell line ...
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Discrimination of Potent Inhibitors of Toxoplasma gondii Enoyl-Acyl Carrier Protein Reductase by a Thermal Shift Assay
... activity assay. A thermal shift assay was employed to further characterize these compounds as well as 14 other potent inhibitors from previous ...
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Fragment Screening at Adenosine-A3 Receptors in Living Cells Using a Fluorescence-Based Binding Assay
... Binding Assay CHO CRE-SPAP cells stably expressing the A 3 AR or A 3 -YFP or CHO A1 cells were seeded into the central 60 wells of a 96-well clear-bottomed, black- walled plate and grown to ...
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Fluorescence Intensity Assay Based on Suppression of Daphnia Magna Feeding on Dry Algae (Chlorella and Spirulina)
... and fluorescence of unconsumed algal particles ...Thus, fluorescence of algal food particles was measured in assays of normally filtering control ...and fluorescence intensity of unfiltered algal ...
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A Phos Tag Based Fluorescence Quenching System for Activity Assay and Inhibitor Screening for Alkaline Phosphatase
... and 2.5 µM for an AP from Escherichia coli at pH 8.0 [19], both determined by means of a spectrophotometric kinetic assay with 4-nitrophenyl phosphate as a substrate. Differences in the IC 50 values appeared to ...
10
Development of a Novel Efficient Fluorescence-Based Plaque Reduction Microneutralization Assay for Measles Virus Immunity
... kits based on a PRN titer of 120 as a cutoff (12, ...new assay, we used low, moderate, and high MV vaccine responders’ sera as determined by the Dade Behring ...new assay was sensitive enough to ...
6
Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay
... the fluorescence value of a control sample that is identical to the test sample, except the GFP gene has been disabled by ...turbidity based on their fluorescence ...
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Performance of coumarin derived dendrimer based fluorescence linked immunosorbent assay (FLISA) to detect malaria antigen
... of a large number of non-infectious donors is considered as one goal for prevention [30]. For this reason, coumarin- derived dendrimer-based FLISA assay was developed and compared to conventional ELISA. ...
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Dye- and fluorescence-based assay to characterize symplastic and apoplastic trafficking in soybean (Glycime max L.) endosperm
... tabacina (Hsieh et al. 2001). Hence, soybean endosperm is common in morphological and structural features in the genus Glycine. We also described that endosperm is easily peeled from maturing soybean seed, thus this is ...
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A plasmonic thermal sensing based portable device for lateral flow assay detection and quantification
... tection. Thermal sensing generates heat by the nano- particles on the entire test ...the thermal sensing is more stable and ...while thermal sensing is an indirect measurement of the concentration of ...
12
Fluorescence Resonance Energy Transfer-Based Intracellular Assay for the Conformation of Hepatitis C Virus Drug Target NS5A
... Given the number of different antiviral compounds that act on one or more forms of NS5A and may soon be in the clinic, we feel this assay may shed new insight into the mechanistic details of how these compounds ...
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Fluorescence based incision assay for human XPF ERCC1 activity identifies important elements of DNA junction recognition
... real-time fluorescence-based assay to measure endonuclease ...this assay, we show that changes in the sequence of the duplex upstream of the incision site results in up to 100-fold variation ...
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Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease
... existence of aggregated recombinant protease species. Nevertheless, they determined that the different peak fractions did not differ in enzymatic activity [34]. Our study used direct plotting of kinetics data by non- ...
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Improving Thermal Safe Test Scheduling for Core Based Systems on Chip Using Shift Frequency Scaling
... a thermal-safe test scheduling approach ...scan shift frequency scaling on the thermal-safe test scheduling performance and propose an algorithm which embeds shift frequency scaling into the ...
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Homogeneous time-resolved G protein-coupled receptor–ligand binding assay based on fluorescence cross-correlation spectroscopy
... GPCRs, assay formats have been so far mainly limited to cell- based functional assays and radioligand binding ...FCCS assay format for a robust, high-throughput determination of receptoreligand ...
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Detection, Differentiation, and Quantitation of Pathogenic Leishmania Organisms by a Fluorescence Resonance Energy Transfer Based Real Time PCR Assay
... an assay must be accurately ...and thermal cycling profile, and second, the analytical sensitivity of the complete procedure including ex- traction of ...
7
Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay
... in fluorescence complementation assays, fluorophore maturation causes a delay between fusion proteins interaction and the appearance of ...permits fluorescence maturation as long as the split Venus ...
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