Time-Resolved Fluorescence and Imaging
The use of time resolved fluorescence imaging in the study of protein kinase C localisation in cells
... bulk fluorescence measurements, the signal is necessarily relatively weak, contributions from back- ground and other factors must be ...the imaging approach to be ...Lifetime imaging of GFP-PKC ...
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A CMOS time-resolved fluorescence lifetime analysis micro-system
... at fluorescence lifetime imaging, with a 256 × 256 pinned photodiode array is implemented in a ...[27], fluorescence decay data is calculated by varying the time at which the photodiode is ...
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Time resolved studies of the photodissociation of adenine
... vacuum imaging detector (VID), or MCP when performing VMI or TR-MS measurements respectively (a simple MCP set-up was initially used prior to installation of ...the fluorescence signal from the VID, due to ...
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Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays
... Both time-domain and frequency-domain instrumentation systems are available to acquire FLIM ...proposed time-domain approaches and discuss how they can be applied to the latest solid-state sensor arrays for ...
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Single-shot time-gated fluorescence lifetime imaging using three-frame images
... the fluorescence lifetime images with a single excitation to freeze the instantaneous ...Conventional time-gated FLI measures the fluorescence delay functions by gating the image intensifier of an ...
20
Dual wavelength excitation for the time-resolved photoluminescence imaging of painted ancient Egyptian objects
... photographic imaging, PL spec- troscopic imaging techniques have found multiple appli- cations for the analysis of paintings, including those carried out with a scanning Lidar approach [10, 11] and with ...
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Density measurement of OH radicals in non-thermal plasmas by laser induced fluorescence and time-resolved absorption spectroscopy
... long time, it is crucial to avoid changes of the output wavelength of the dye ...41AU02.AS, Imaging Source) are used to record the interference ...exposure time of the CCD is set to 300 ms, shots are ...
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Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... Photoisomerization might offer a route to activation of the low activity E-combretastatin to the higher activity Z-isomer. Light activation of drugs has been proposed to have the advantages of temporal and spatial ...
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Nucleosome dynamics resolved with single-pair fluorescence resonance energy transfer spectroscopy
... single-molecule fluorescence microscopy several immobilization schemes have been employed successfully (reviewed by Rasnik [19]), such as specific tethering to BSA-biotin [20] or polyethylene glycol (PEG) [21] ...
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Time-resolved tympanal mechanics of the locust
... The locust tympanal membrane uses a travelling wave as method for spatial frequency analysis. The membrane is shown to react differently to particular frequency ranges through frequency domain analysis. These results ...
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Time-resolved imaging of magnetic vortex dynamics using holography with extended reference autocorrelation by linear differential operator
... the imaging is performed at 45°, providing the required non-zero in-plane projection of the x-ray wave ...of-normal imaging are related to the angle of ...
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Probing the origins of vibrational mode specificity in intramolecular dynamics through picosecond time resolved photoelectron imaging studies
... In all three cases (see Fig. 4), oscillations in the peak intensities are seen to occur during the first 6 ps. In the two pFT cases (Figs. 4a, 4b), the intensities of peaks A, B and E decrease between 0 and 2 ps whilst ...
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Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time resolved fluorescence based hybridization assay
... In this assay format, the PCR is run by standard procedures and the subsequent hybridization reaction is carried out in solution by using two oligonucleotide probes, one biotinylated and[r] ...
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Measurement of infection efficiency of a major wheat pathogen using time-resolved imaging of disease progress
... same time, colony densities did not differ significantly between reference membranes that belonged to the same treatment indicating that the density of spores was the same on reference ...
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Time Resolved Fluorescence Investigation of the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein: Influence of the Binding of Nucleic Acids
... the fluorescence lifetimes and intensities recovered by MEM analysis on the reconstructed decay are in excellent agreement with the real ones (Table ...life- time distribution, which is significantly ...
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Resolved imaging of the AR puppis circumbinary disk
... Circumbinary disks are common around post-asymptotic giant branch ( post-AGB ) stars with a stellar companion on orbital timescales of a few 100 to few 1000 days. The presence of a disk is usually inferred from the ...
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Development of Single and Multimodality Imaging Probes for PET, SPECT and Fluorescence Imaging
... This dissertation is in an integrated format discussing three major projects centered around probe development. In the first project, novel metal-chelated and fluorinated GLP-1 derivatives were prepared all containing ...
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Postoperative Assessment of Extracranial–Intracranial Bypass by Time Resolved 3D Contrast Enhanced MR Angiography Using Parallel Imaging
... We obtained CE-MRA images from 17 consecutive patients referred to us for MR imaging following EC-IC bypass during a 2-year period. The subjects consisted of 7 men and 10 women aged 23–74 years (mean age, 51.2 ...
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Time-resolved X-ray absorption spectroscopy
... the time structure of the laser ...implemented time- resolved X-ray absorption spectroscopy in a pump–probe geometry (Figure 3), with the pump laser synchronised to the radiofrequency cavity of the ...
5
Towards time resolved single spin measurements.
... To start, we assume to be able to create an experimental environment sim- ilar to the conditions used in Baumann et al [3]. This means that we try to create a system Rabi oscillating between two levels split by a gap of ...
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