tissue microarrays
Immunoprofile from tissue microarrays to stratify familial breast cancer patients
... Familial breast cancer (BC) is a heterogeneous disease with variable prognosis. The identification of an immunoprofile is important to predict tumor behavior for the routine clinical management of familial BC patients. ...
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The clinicopathologic association of c-MET overexpression in Iranian gastric carcinomas; an immunohistochemical study of tissue microarrays
... The tissue microarrays (TMA) were constructed as described previously [32]. In each case, 5-μm H & E slides were used to identify and mark out representative areas of tumor tissue. From each ...
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Segmentation on Ovarian Cancer Tissue Microarrays using FWMS and NCuts Algorithm
... ABSTRACT: Quantifying the presence and extent of staining on account of a vascular biomarker on tissue microarrays is a laborious, time consuming and error prone job. We demonstrate our flexible, robust, ...
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An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips
... Manual Tissue Arrayers are commercially available ...a tissue array block in minutes, simply by punching the donor tissue cores using punch needles with plunger and insert to the pre-made paraf- fin ...
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How to make tissue microarrays
... It is now almost 20 years since tissue microarrays (TMAs) were first described by Kononen and colleagues. Today, this high throughput methodology has been widely adopted by investigators who require a ...
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Performance of automated scoring of ER, PR, HER2, CK5/6 and EGFR in breast cancer tissue microarrays in the Breast Cancer Association Consortium.
... tant. Tissue microarrays (TMAs) can be used to assess IHC results for multiple cases in one tissue section [10], enabling standardized IHC staining and facilitating ...
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The development and validation of the Virtual Tissue Matrix, a software application that facilitates the review of tissue microarrays on line
... Tissue Microarrays (TMAs) provide high-throughput anal- ysis of tissue samples for in situ hybridisation and immu- nohistochemistry, by means of arranging multiple tissue samples in a uniform ...
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Ki67 expression in invasive breast cancer: the use of tissue microarrays compared with whole tissue sections
... using tissue microarrays (TMA) platform [14] [15], although it remains unclear as to their validity and comparison with assessment in whole tissue sections ...
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The adequacy of tissue microarrays in the assessment of inter- and intra-tumoural heterogeneity of infiltrating lymphocyte burden in leiomyosarcoma.
... Optimal number of cores to ensure representativeness of tissue microarrays depends on required degree of accuracy. To address the question of how many TMA cores must be sampled from a tumour to provide ...
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Tissue microarrays: applications in study of p16 and p53 alterations in Ewing's cell lines
... Tissue microarrays (TMAs) are becoming the standard for the validation of prognostic biomarkers; this new technol- ogy leads to savings of time, cost and tissues ...embedded tissue samples arrayed on ...
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Membrane connectivity estimated by digital image analysis of HER2 immunohistochemistry is concordant with visual scoring and fluorescence in situ hybridization results: algorithm evaluation on breast cancer tissue microarrays
... tumour tissue sampling for the analysis. If non-tumour tissue is included in the analysis, it may “dilute” the per- centage of positive ...tumour tissue was per- formed, nevertheless, the DA ...
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The usefulness of immunohistochemistry in tissue microarrays of Human Papillomavirus negative adenocarcinoma of the uterine cervix
... of tissue microarray techniques allowed uniform conditions during immuno- histochemical staining, making the results more compar- able, and all specimens were tested for HPV using sensitive ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... in tissue sections (in situ ...of tissue structures and can be carried out on only one or a few sections per experiment, resulting in low ...available tissue section microarrays enabling the ...
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In vitro tissue microarrays for quick and efficient spheroid characterisation
... Three-dimensional (3D) in vitro microphysiological cultures, such as spheroids and organoids, promise increased patient relevance and therapeutic predictivity compared with reductionist cell monolayers. However, ...
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Tissue microarrays: one size does not fit all
... mm tissue cores were more appropriate than ...tumor tissue, core folding, and necrotic ...whole tissue sections did not allow us to address additional variability that can be introduced by day-to-day ...
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Tissue microarrays analysis in chondrosarcomas: light microscopy, immunohistochemistry and xenograft study
... of Tissue Microarray (TMA) technology offers the advantage to screening large tissue cohorts for biomarker expression and to examine serial sections obtained from the same tumour specimen and xenograft ...
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Identification of tumor epithelium and stroma in tissue microarrays using texture analysis
... tumor tissue have, to some extent, been previously studied, but generally included only small specimen ...static tissue components in large-scale histological scenes was feasible using Haralick ’ s ...
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PubMedCentral-PMC5025359.pdf
... human tissue microarrays to explore the utility of SIX1 as a biomarker in human endometrial ...endometrial tissue specimens, SIX1 was not present in normal endometrium but was expressed in a subset ...
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Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer
... Tissue microarrays were immunohistochemically labeled using a polyclonal antibody directed against a unique region of RRBP1. The immunogen used was a recombinant peptide of 107 amino acids that constitutes ...
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Is TIMP 1 immunoreactivity alone or in combination with other markers a predictor of benefit from anthracyclines in the BR9601 adjuvant breast cancer chemotherapy trial?
... For the current analysis, following approval by the central ethics committee (South East Multi-centre Research Ethics Committee), tissue blocks were retrieved from 321 cases (85.8%) among which 288 cases (77.0%) ...
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