TOPOISOMERASE-II
Assessment of drug induced genotoxicity in mammalian cells and the contribution of topoisomerase II inhibition
... highest concentrations analysed in the ICE bioassay. Etoposide gave a LOCCEL of 0.1 µmol/L. This concentration was relatively non-cytotoxic, yielding a RPD of 92%. The LOCCEL for gemifloxacin was 100 µmol/L, and this ...
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Hypersensitive Inhibition of the Proliferation of Cells with Mutated DNA Repair Related Genes by the Catalytic Topoisomerase II Inhibitor 20 O IngenolEZ
... Topoisomerase II (topo II) is essential for the survival of proliferating cells, and it is involved in recombination, chromosome condensation, and decatenation of sister chromatids before anaphase of ...
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cGAS/STING axis mediates a topoisomerase II inhibitor–induced tumor immunogenicity
... designed a T cell activation assay as well as an HMGB1–Gaussia luciferase (HMGB1-Gluc) reporter assay. In the T cell activation assay, T cells and DCs were cocultured with tumor cells pretreat- ed with different drugs, ...
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Inhibition of topoisomerase II by ICRF-193 prevents efficient replication of herpes simplex virus type 1.
... own topoisomerase, it is reasonable to assume that a cellular topoisomerase will play an active role during the synthesis of viral DNA ...somerase II to a closed clamp unable to cleave DNA ...and ...
7
The ATP dependent chromatin remodelling enzyme Uls1 prevents Topoisomerase II poisoning
... Topoisomerase II (Top2) is an essential enzyme that decatenates DNA via a transient Top2-DNA cova- lent intermediate. This intermediate can be stabilized by a class of drugs termed Top2 poisons, resulting ...
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Clinicopathologic study of E cadherin/beta catenin complex, and topoisomerase II in a series of 71 liposarcoma cases
... and topoisomerase II alpha was examined immunohistochemically on formalin-fixed paraffin-embedded tissue specimens from 71 patients who underwent surgical treatment for liposarcomas of the extremities or ...
6
Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a / mutants
... top2a mutants exhibit defects in cell cycle progression Topoisomerase II alpha genes are known to be critical for cell cycle progression. Therefore, we quantified, by flow cytometry, the distribution of blm ...
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Vaccinia Virus DNA Ligase Recruits Cellular Topoisomerase II to Sites of Viral Replication and Assembly
... human topoisomerase II (gi ...protein topoisomerase II ␣ (gi 19913406; 68% amino acid identity from topoisomerase II  residues 901 to 1260 and 34% from residue 1261 to ...
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Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a(-/-) mutants
... topoisomerases II paralogues are also important as targets of anti-cancer ...blocks topoisomerase II-mediated ATP hydrolysis which is required to regenerate its active enzymatic form ...a ...
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Targeting of Chk2 as a countermeasure to dose-limiting toxicity triggered by topoisomerase-II (TOP2) poisons
... with topoisomerase II (TOP2)–inhibitors whereas no such protection was observed following treatment with topoisomerase I (TOP1) ...Set II (NCI/NTP) chemical library we identified the ...
11
Topoisomerase II trapping agent teniposide induces apoptosis and G2/M or S phase arrest of oral squamous cell carcinoma
... cancers. Topoisomerase II (Topo II) exists ubiquitously in cells and plays an essential role in DNA replication, transcrip- tion, chromosome formation and separation of sister chromatid ...Topo ...
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The SMC Complex MukBEF Recruits Topoisomerase IV to the Origin of Replication Region in Live Escherichia coli
... An earlier study of TopoIV in E. coli found that the catalytic subunit, ParC, was associated with the replisome, while the other subunit, ParE, was located in nucleoid-free regions of the cell (31). Based on this and ...
10
Regulation of DNA Damage Signaling and Cell Death Responses by Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) and LMP2A in Nasopharyngeal Carcinoma Cells
... Nasopharyngeal carcinoma (NPC) is closely associated with latent Epstein-Barr virus (EBV) infection. Although EBV infection of preneoplastic epithelial cells is not immortalizing, EBV can modulate oncogenic and cell ...
13
Comparative evaluation of three proliferation markers, Ki-67, TOP2A, and RacGAP1, in bronchopulmonary neuroendocrine neoplasms: Issues and prospects
... Ki-67, Topoisomerase II alpha (TOP2A), and RacGAP1, in a series of tumor samples from 104 BP-NEN patients (24 TC, 21 AC, 52 SCLC, 7 LCNEC) using different evaluation methods (immunohistochemistry (IHC): ...
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Topoisomerase II Inhibitors Induce DNA Damage Dependent Interferon Responses Circumventing Ebola Virus Immune Evasion
... Doxorubicin and daunorubicin induce IFN by an ATM-dependent mechanism. Doxorubicin has pleiotropic effects on cells. Among its activities, it is a topoisomerase II poison that intercalates into DNA, ...
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Selective induction of apoptosis in Hep 3B cells by topoisomerase I inhibitors: evidence for a protease dependent pathway that does not activate cysteine protease P32
... whether topoisomerase-directed drugs would in- duce apoptosis in a human HCC cell line (Hep 3B) and, if so, to investigate the ...The topoisomerase I poi- son camptothecin (CPT) induced apoptosis of Hep 3B ...
10
A role for topoisomerase II alpha in chromosome damage in human cell lines
... topo II action involves DSB induction, the DSBs are protein-associated and the DSB is usually religated soon after, so no overall damage will have ...topo II might mis-join the ends of the ...topo II ...
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Topoisomerase I and RecQL1 Function in Epstein-Barr Virus Lytic Reactivation
... therapies. Topoisomerase I (Topo I) and Topo II inhibitors can selectively inhibit Epstein-Barr virus (EBV) lytic cycle DNA ...Topo II inhibitor etoposide are potent inhibitors of the transcription ...
9
Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders
... polymerase II as well as topoisomerase I and II proteins ...ase II and TOP2 ...polymerase II inhibitor alpha-amanitin; ...by topoisomerase inhibition when transcrip- tion is ...
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