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total internal reflection microscopy

Total internal reflection fluorescence microscopy for characterizing biochemical interactions

Total internal reflection fluorescence microscopy for characterizing biochemical interactions

... the glycines and other residues. 91 The X and Y residues are exposed on the surface of the molecule and are frequently proline or hydroxyproline, respectively. These residues also stabilize the triple helix by ...

167

Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana

Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana

... Because the TIRFM technique is unrivaled in its ima- ging capability in the near-boundary field, it has been successfully applied to in vivo studies of animal cells for the past decade [3,4]. However, the thick cell wall ...

7

Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy

Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy

... with total internal reflection fluorescence (TIRF) microscopy while catecholamine release from single vesicles was measured as amperometric spikes with the surface patterned ...

11

Development of Optical Biosensors Based on Metal Nanostructures for Pollution (Mycotoxins) Detection

Development of Optical Biosensors Based on Metal Nanostructures for Pollution (Mycotoxins) Detection

... electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), optical absorption spectroscopy, and spectroscopic ...of total internal reflection ellipsometry (TIRE) ...

204

Madhira, Sushi
  

(2017):


	Single-molecule multi-color FRET: from DNA to protein.


Dissertation, LMU München: Fakultät für Chemie und Pharmazie

Madhira, Sushi (2017): Single-molecule multi-color FRET: from DNA to protein. Dissertation, LMU München: Fakultät für Chemie und Pharmazie

... with total internal reflection fluorescence microscopy (TIRFM) allows monitoring the dynamic interconversion of states of labeled biomolecules, immobilized on the surface, for as long as the ...

168

5431.pdf

5431.pdf

... fluorescence microscopy-based technique, total internal reflection – fluorescence correlation spectroscopy (TIR-FCS), can also be used to study the thermodynamics and kinetics of interacting ...

158

Single Molecule investigations of DNA Looping Using the Tethered Particle Method and Translocation by Acto-Myosin Using Polarized Total Internal Reflection Fluorescence Microscopy

Single Molecule investigations of DNA Looping Using the Tethered Particle Method and Translocation by Acto-Myosin Using Polarized Total Internal Reflection Fluorescence Microscopy

... polarized total internal reflection (polTIRF) microscopy, a technique that determines the orientation and wobble of a single fluorophore attached to the bio-molecule of ...

264

Bayesian multi-target tracking: application to total internal reflection fluorescence microscopy

Bayesian multi-target tracking: application to total internal reflection fluorescence microscopy

... The main technique to deal with Poisson process noise are the variance stabiliz- ing transform (VST) methods. One of most applicable VST methods is generalized Anscombe transform (GAT) which converts a Poisson process to ...

146

Research and development in optical biosensors for determination of toxic environmental pollutants

Research and development in optical biosensors for determination of toxic environmental pollutants

... Low molecular weight (LMW) environmental toxins, such as simazine, atrazine, nonylphenol and T-2 mycotoxin were registered with the methods of surface plasmon resonance (SPR) and the recently developed total ...

280

lenses and refraction.docx

lenses and refraction.docx

... Total internal reflection occurs when the angle of incidence in the denser of the two media is greater than the critical angle and light is reflected back into the denser medium.. The [r] ...

10

Protein diffusion in Escherichia coli

Protein diffusion in Escherichia coli

... The cell length L was measured by using confocal microscopy and E. coli treated with the membrane stain FM1-43. Sixteen 36.6-µm by 36.6-µm images were collected in the x-y plane by moving away from the coverslip ...

162

An active terahertz polarization converter employing vanadium dioxide and a metal wire grating in total internal reflection geometry

An active terahertz polarization converter employing vanadium dioxide and a metal wire grating in total internal reflection geometry

... state. In this experiment, the s-reflection can be regarded as a combination of the s-reflections by the s-input component and the p-input component. Theoretically, these two reflections are fully out of phase, ...

10

Design study and Modeling of Surface Plasmon Resonance (SPR)-based Optical Fiber Sensor Utilizing Zinc Oxide and Gold Layers

Design study and Modeling of Surface Plasmon Resonance (SPR)-based Optical Fiber Sensor Utilizing Zinc Oxide and Gold Layers

... attenuated total reflection (ATR) with Kretschmann’s ...makes total internal reflection at the metal-dielectric interface, the evanescent waves excite the surface plasmons on the metal ...

7

Reflection of internal waves by sand ripples

Reflection of internal waves by sand ripples

... In the present paper an attempt has been made to determine the transmission and reflection coefficients for the diffracted waves along the interface of two laterally unbounded superposed[r] ...

7

A Simple Analytic Approximation for the Refracted Field at Gaussian Beam Incidence upon a Boundary of Absorbing Medium

A Simple Analytic Approximation for the Refracted Field at Gaussian Beam Incidence upon a Boundary of Absorbing Medium

... field propagation and exponential decay also does not take place for the refracted beam when the incident angle passes the critical value [27]. Really, in the spectrum of the beam (7), there are plane waves with various ...

9

Characterisation of thin films of graphene–surfactant composites produced through a novel semi automated method

Characterisation of thin films of graphene–surfactant composites produced through a novel semi automated method

... of total internal reflection ellipsometry (TIRE) which revealed the enhancement of the surface plasmon resonance in thin gold films by depositing graphene ...

12

Point of Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology

Point of Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology

... noassay method shown in Figure 2. When the samples were added, the sample rehy- drated the trace mAb and magnetic particle in the detection area and then the antigen action with the composites. The bidirectional magnetic ...

6

Polymer Optical Fiber Sensor and the Prediction of Sensor Response Utilizing Artificial Neural Networks.

Polymer Optical Fiber Sensor and the Prediction of Sensor Response Utilizing Artificial Neural Networks.

... The lowest pressure applied by a seated human ideally takes place on the thigh region (Figure 2.26 in 2.3.2) of a seat cushion according to the existing literature (S. Drummond et al., 1982; Kurz, Diebschlag & ...

274

Nonlinear interfaces: intrinsically nonparaxial regimes and effects

Nonlinear interfaces: intrinsically nonparaxial regimes and effects

... As regards numerical investigations, novel methods have been developed to understand nonlinear phenomena governed by the NLH equation, where backscattered waves can accompany a forward propagating beam. Leaving aside the ...

10

Introduction to fiber optics: Sensors for biomedical applications

Introduction to fiber optics: Sensors for biomedical applications

... undergo total internal reflection and aid in possible future biomedical applications to carry out the simultaneous collection and analysis of samples for drug safety ...

6

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