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Total internal reflection microscopy (TIRF)

Total internal reflection fluorescence microscopy for characterizing biochemical interactions

Total internal reflection fluorescence microscopy for characterizing biochemical interactions

... through-objective TIRF microscopes, combined with improvements in data acquisition rates in electron-multiplying charge coupled device (EM-CCD) cameras, has lead to a rapid rise in use of TIRF in single- ...

167

Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy

Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy

... with total internal reflection fluorescence (TIRF) microscopy while catecholamine release from single vesicles was measured as amperometric spikes with the surface patterned ...

11

Bayesian multi-target tracking: application to total internal reflection fluorescence microscopy

Bayesian multi-target tracking: application to total internal reflection fluorescence microscopy

... Knowledge of the clutter rate and detection profile of the chosen detection method, are of critical importance in Bayesian multi-target tracking. Most existing multi- target tracking solutions such as what we proposed in ...

146

Single Molecule investigations of DNA Looping Using the Tethered Particle Method and Translocation by Acto-Myosin Using Polarized Total Internal Reflection Fluorescence Microscopy

Single Molecule investigations of DNA Looping Using the Tethered Particle Method and Translocation by Acto-Myosin Using Polarized Total Internal Reflection Fluorescence Microscopy

... polarized total internal reflection (polTIRF) microscopy, a technique that determines the orientation and wobble of a single fluorophore attached to the bio-molecule of ...

264

Madhira, Sushi
  

(2017):


	Single-molecule multi-color FRET: from DNA to protein.


Dissertation, LMU München: Fakultät für Chemie und Pharmazie

Madhira, Sushi (2017): Single-molecule multi-color FRET: from DNA to protein. Dissertation, LMU München: Fakultät für Chemie und Pharmazie

... with total internal reflection fluorescence microscopy (TIRFM) allows monitoring the dynamic interconversion of states of labeled biomolecules, immobilized on the surface, for as long as the ...

168

Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana

Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana

... As shown in Figure 2C and 2D, a laser light beam can penetrate the aqueous medium under fTIR conditions. Thus, the first major obstacle to the application of TIRFM to plant-cell studies is overcome. If we consider the n ...

7

5431.pdf

5431.pdf

... fluorescence microscopy-based technique, total internal reflection – fluorescence correlation spectroscopy (TIR-FCS), can also be used to study the thermodynamics and kinetics of interacting ...

158

Development of Optical Biosensors Based on Metal Nanostructures for Pollution (Mycotoxins) Detection

Development of Optical Biosensors Based on Metal Nanostructures for Pollution (Mycotoxins) Detection

... electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), optical absorption spectroscopy, and spectroscopic ...of total internal reflection ellipsometry (TIRE) ...

204

Point of Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology

Point of Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology

... We have presented a three dimensional optical protein chip that fulfills the demand- ing for point-of-care diagnostics in terms of ease-of-use (one step assay), miniaturi- zation (5 μl). The artful combination of ...

6

Characterisation of thin films of graphene–surfactant composites produced through a novel semi automated method

Characterisation of thin films of graphene–surfactant composites produced through a novel semi automated method

... The spectroscopic ellipsometry study of graphene thin films gave similar values for the thickness of the graphene–surfactant composite layer. While the dispersions of refractive index and extinction coefficient were ...

12

Recap_OFC_19_08_14.pptx

Recap_OFC_19_08_14.pptx

...  High index guiding fibers are guiding light in a solid core. by the Modified Total Internal Reflection (M-TIR) principle[r] ...

35

Total  Reflection of  Self  Concept

Total Reflection of Self Concept

... In the early stage of interaction with reference group, whether the reference is the primary group ( i.e the family for young child) or a secondary (i.e one’s peer or co-workers), choices and decisions are channeled ...

10

11 Total Iinternal Reflection

11 Total Iinternal Reflection

... In the worksheet ’11.8 Support: Refraction and Snell’s law’ you practised using Snell’s law in refraction problems. In this worksheet you will meet the concept of total internal reflection and the ...

6

Introduction to fiber optics: Sensors for biomedical applications

Introduction to fiber optics: Sensors for biomedical applications

... by total internal reflection is that the transmitting fiber must be coated with the material that has refractive index smaller (Cladding material) than that of fiber material (Core) as shown in the ...

6

Graphene loaded metal wire grating for deep and broadband THz modulation in total internal reflection geometry

Graphene loaded metal wire grating for deep and broadband THz modulation in total internal reflection geometry

... A reflection THz time-domain spectroscopy system from Menlo Systems (TERA-K15) was used for the TIR geometry experiments with the THz incident at 30° to the normal ...

11

Protein diffusion in Escherichia coli

Protein diffusion in Escherichia coli

... The cell length L was measured by using confocal microscopy and E. coli treated with the membrane stain FM1-43. Sixteen 36.6-µm by 36.6-µm images were collected in the x-y plane by moving away from the coverslip ...

162

Design study and Modeling of Surface Plasmon Resonance (SPR)-based Optical Fiber Sensor Utilizing Zinc Oxide and Gold Layers

Design study and Modeling of Surface Plasmon Resonance (SPR)-based Optical Fiber Sensor Utilizing Zinc Oxide and Gold Layers

... attenuated total reflection (ATR) with Kretschmann’s ...makes total internal reflection at the metal-dielectric interface, the evanescent waves excite the surface plasmons on the metal ...

7

Reflection of internal waves by sand ripples

Reflection of internal waves by sand ripples

... In the present paper an attempt has been made to determine the transmission and reflection coefficients for the diffracted waves along the interface of two laterally unbounded superposed[r] ...

7

Research and development in optical biosensors for determination of toxic environmental pollutants

Research and development in optical biosensors for determination of toxic environmental pollutants

... Low molecular weight (LMW) environmental toxins, such as simazine, atrazine, nonylphenol and T-2 mycotoxin were registered with the methods of surface plasmon resonance (SPR) and the recently developed total ...

280

5449.pdf

5449.pdf

... (A) TIRF image of a single filopodium from a HeLa cell expressing GFP-Myo10 showing a bright punctum of GFP-Myo10 at the tip of the filopodium, several faint particles of GFP-Myo10 along the shaft, and diffuse ...

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