Transformation of Escherichia coli with plasmid DNA
Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism
... Results: Escherichia coli cells, either devoid of plasmid DNA or bearing plasmid pOri1 (with a single ColE1 replication origin) or plasmid pOri2 (with double ColE1 replication ...
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Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode
... Plasmid DNA (pDNA) is an attractive alternative for immunization and gene therapy against many infectious, genetic and acquired diseases ...bacterium Escherichia coli. Several E. coli ...
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Destruction of Escherichia coli and Broad Host Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions
... broad-host-range plasmid and chromosomal DNA in simulated ...to Escherichia coli (uidA) and IncP broad-host-range plasmids (trfA) were monitored using real-time quantitative polymerase chain ...
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Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA* (CaCI2/extrachromosomal DNA/plasmid)
... Addition of DNase to reaction mixtures before the heat pulse (420) also prevented transformation but had no effect on expression of antibiotic resistance when added after the 2-min incub[r] ...
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LAB 16 Rapid Colony Transformation of E. coli with Plasmid DNA
... 14. Transformation efficiency is expressed as the number of transformed bacteria per microgram of DNA ...Because transformation is limited to only those cells that are competent (in the stationary ...
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Effect of human polymorphonuclear and mononuclear leukocytes on chromosomal and plasmid DNA of Escherichia coli Role of acid DNase
... of Escherichia coli DNA following phagocytosis of E. coli by polymorphonuclear and mononuclear ...E. coli PC2166 and E. coli 048K1 were incubated in serum, washed, and added to ...
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Transformation of Vegetative Cells of Bacillus thuringiensis by Plasmid DNA
... kurstaki) gave the highest transformation frequency' with Tris hydrochloride buffer containing 35% sucrose. galleriae) was grown in minimal medium supplemented with both Casamino Acids a[r] ...
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Quantification of plasmid DNA reference materials for Shiga toxin-producing Escherichia coli based on UV, HR-ICP-MS and digital PCR
... Based on detailed optimization of 3 different methods, with very different analysis strategies and detection sensi- tivity, we finally combined them for the quantification of DNA reference materials. Due to the ...
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Plasmid segregational stability in 'Escherichia coli'
... E. coli populations is the method of growth prior to their ...of transformation observed variea dramatically throughout the growth cycle, the most susceptible cells being those in the early exponential ...
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Plasmid maintenance in Escherichia coli K 12
... of plasmid DNA replication events are initiated, presumably leading to bidirectional replication in a similar manner to that of the E ...E.coli DNA polymerase I, but ...
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Escherichia coli Plasmid Copy Number Assay
... DNA was prepared from samples using Qiagen genomic tips, using the method described above. Smaller scale columns are generally more appropriate for samples. Qiagen Tip 20Gs have been used successfully for samples, ...
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Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin
... E. coli BW- ASTA under this condition with induction of the IPTG- controlled heterologous promoters led in the early phase of the cultivation to the formation of astaxanthin and the intermediates adonirubin and ...
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A DNA Vaccine against Escherichia coli O157:H7
... stranded DNA, more controversy is seen in the effect of plasmid ...of DNA were used in intramuscular immunization with no prior treatment or adjuvant addition in order to achieve maximal immune ...
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Local DNA Sequence Control of Deletion Formation in Escherichia coli Plasmid pBR322
... that the reversion of insertion mutations induced by the Tn5-related inverse transposon diagrammed in Figure 2 is due to deletion of the inserted segment plus a c[r] ...
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Environmental and genetic determinants of plasmid mobility in pathogenic Escherichia coli
... The experimental and outgrowth conjugation control curves must be displaced in time; otherwise, OD will not reach target OD 600 thresholds. For this reason, all cell mixtures were diluted 150× in M9CA with dual ...
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Selection and Plasmid Transfer Underlie Adaptive Mutation in Escherichia coli
... of Escherichia coli is plated on lactose medium, where the nondividing population gives rise to Lac + revertant colonies during a week under ...F9lac plasmid that also encodes the error-prone ...
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Environmental and genetic determinants of plasmid mobility in pathogenic Escherichia coli
... of plasmid mobility to fight antibiotic ...E. coli pathogens from patient bloodstream infections, we screened for the ability to transfer -lactam resistance commonly found on plasmids native to the ...
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THE INFIDELITY OF CONJUGAL DNA TRANSFER IN ESCHERICHIA COLI
... Quite similar spectra for amber mutations are obtained with or without F’ transfer, the exception being an increase in the proportion of mutants isolated at the mutat[r] ...
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Plasmid-mediated colistin resistance in Escherichia coli from the Arabian Peninsula
... IncHI2 plasmid carrying mcr-1 and also resistance genes to several other classes of antibiotics, from a human ...mcr-1 plasmid was first identified 30 by probing the contigs in GenBank in a ...E. coli ...
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Plasmid Mediated Fluoroquinolone Resistance Determinants in Clinical Isolates of Escherichia coli
... Escherichia coli is a gram-negative bacterium that causes a multitude of infection such as urinary tract infections, gastroenteritis, skin and soft tissue infections and bacteremia ...
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