Transient Gene Expression
13. Establishing Agrobacterium mediated transient gene expression assay in Nicotiana tabacum
... transient expression could be thousand times more powerful than in stable ...ectopic gene expression studies in plants namely electroporation or, polyethylene glycol (PEG) based transformation ...
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A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings
... for transient gene expression in ...of transient GUSPlus ...GUS expression were sonication, needle wounding, mixing by vortex with carborundum, separa- tion by centrifugation and heat ...
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Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells
... Transient gene expression (TGE) is capable of producing recombinant proteins (rProteins) faster and cheaper than the standard stable cell line development process, making it an attractive alternative ...
7
Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana
... transgene expression was controlled by the constitutive Cauliflower mosaic virus (CaMV) 35S promoter and the transgene mRNA was engineered to include translation enhancer sequences derived from Cowpea mosaic virus ...
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Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies
... Background: Transient gene expression systems are indispensable tools in molecular ...transgene expression is detectable within 90 minutes of transformation and lasts for several ...
12
An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves
... To establish an efficient method for Agrobacterium in- fection of rice leaves, we tested different A. tumefaciens strains (GV2200, EHA105, AGL1, LBA4404, and GV3101) carrying the same binary plasmid as well as different ...
12
Molecular analysis of anthocyanin biosynthetic pathway genes in Cymbidium orchids : a thesis presentation in partial fulfilment of the requirements for the degree of Master of Science in Plant Molecular Biology at Massey University, Palmerston North, New Zealand
... Transient gene expression studies showed that the use of a heterologous flavonoid biosynthetic gene, dihydroflavonol reductase DFR from Anthurium, enhanced production of pelargonidin in [r] ...
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A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)
... a transient transformation system for gene characterization in upland ...perform gene characterization ...and transient gene expression was observed 5 days after infection of the ...
11
Agrobacterium-mediated transformation systems of Primula vulgaris
... in transient overexpression experiments in Catharanthus roseus in order to study secondary metabolism ...[23]. Transient gene silencing was performed by injecting ...induced gene silencing ...
13
Upstream Regulatory Region Alterations Found in Human Papillomavirus Type 16 (HPV-16) Isolates from Cervical Carcinomas Increase Transcription, ori Function, and HPV Immortalization Capacity in Culture
... Sequence alterations in HPV-16 P97 promoters isolated from cervical carcinomas are correlated with enhanced ori function and increased initial plasmid amplification. We also examined the effects of these ...
10
Murine Gammaherpesvirus 68 Encodes a Second PML-Modifying Protein
... ORF expression plasmids by transient transfection in 3T12 cells, followed by detection of PML localization by immunofluores- cence ...that transient expression of a viral early gene ...
7
Addiction of pancreatic cancer cells to zinc-finger transcription factor ZIC2
... forced expression of FGFR3 enhanced the cellular proliferation in a vimentin- rich “mesenchymal” type of PDAC cell via the MAPK pathway, while it suppressed the cellular proliferation in an E-cadherin-rich ...
12
The neurotensin gene is a downstream target for Ras activation
... the expression of the neurotensin gene (NT/N), a terminally differentiated endocrine product specifically localized in the gastrointestinal tract to the adult small ...NT/N gene and, moreover, that ...
10
Rac1 regulates interleukin 1 induced nuclear factor kappaB activation in an inhibitory protein kappaBalpha independent manner by enhancing the ability of the p65 subunit to transactivate gene expression
... In this study we provide evidence that Rac1 does not lie on the IL1-induced signaling pathway leading to I B ␣ phospho- rylation and degradation, but instead participates in a second process required for NF B ...
7
Tightly controlled MRTF A activity regulates epithelial differentiation during formation of mammary acini
... determined expression and localization of integrin α5 and integrin α6 to investigate if an integrin switch caused the observed growth ...target gene, whilst integrin α6 is an epithelial marker involved in ...
16
Pegylated IFN α regulates hepatic gene expression through transient Jak/STAT activation
... pegIFN-α2b–induced gene transcription is mainly driven by IFN- stimulated response element motifs during the entire dosing ...of gene transcription at later time points when pegIFN-α2b–induced Jak/STAT ...
15
New amber mutation in a beta thalassemic gene with nonmeasurable levels of mutant messenger RNA in vivo
... beta-thalassemia gene that carries a novel nonsense mutation in a Chinese ...the expression of this cloned gene in a transient expression system demonstrated a 65% decrease in levels of ...
6
Genome wide characterization of Foxa2 targets reveals upregulation of floor plate genes and repression of ventrolateral genes in midbrain dopaminergic progenitors
... the expression level of mRNAs, real-time PCR analyses using Platinum SYBR Green Super Mix (Invitrogen) were performed in triplicate using the 7900 PCR System ...Gapdh–Ct gene tested ), and then relative ...
10
In vivo and in vitro analyses of recombinant baculoviruses lacking a functional cg30 gene.
... Furthermore, restriction endonuclease digestion of AcMNPV DNA with SfiI which inactivates cg3O and another AcMNPV gene, pl43, does not affect transient expression from the etl, vp39, or [r] ...
5
Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability
... found evidence of high MOMP abundance at low oxygen conditions (Liu et al., 2012), although another study using 2D-gels with ‘oxygen-acclimated cells’ found higher PorA and CadF at high oxygen conditions (Sulaeman et ...
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