Triplex formation
Triplex formation inhibits HER 2/neu transcription in vitro
... malignancies, contains a purine-rich sequence in its promoter, which is favorable for purine:purine:pyrimidine (R:R:Y) triplex formation. Although its function in the HER-2/neu promoter is unknown, this ...
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Deoxyribonucleic acid triplex formation inhibits granulocyte macrophage colony stimulating factor gene expression and suppresses growth in juvenile myelomonocytic leukemic cells
... lix formation on the transcriptional control elements of the gene of interest with subsequent inhibition of regulatory factor binding, which leads to altered expression ...for triplex-forming ...
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Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide.
... In these experiments, the site-specific triplex formation is designed to deliver the mutagen, psoralen, to the targeted site in the SV40 DNA, long-wavelength UV light UVA; 320 to 400 nm [r] ...
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Recombinant Triplex formed by PNA-TFO: A Molecular Dynamics Simulation Study
... these limitations of triplex formation modification in TFO came into account. Peptide Nucleic Acids (PNA) is artificially designed oligonucleotide with neutral backbone, a mimic of DNA shows Watson-Crick ...
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Selective Inhibition of the Human tie-1 Promoter with Triplex-Forming Oligonucleotides Targeted to Ets Binding Sites
... the triplex-forming oligonucleotide (TFO) to bind in the major groove of double-stranded DNA, forming Hoogsteen hydrogen bonds with the purine strand (reviewed in ...21-23). Triplex DNA formation ...
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Triplex Forming Oligonucleotides – Tool for Gene Targeting
... There are some major obstacles to applications of TFO under physiological conditions. Cellular uptake and deli- very of TFO to target sequence into the nucleus is probably biggest obstacle for successful therapeutic ...
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Assembly of the Herpes Simplex Virus Capsid: Preformed Triplexes Bind to the Nascent Capsid
... for triplex formation or to the lack of sequences involved in at- tachment of the triplex to the major capsid-scaffold protein ...heterotrimeric triplex containing one copy of the truncated ...
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Recognition of GC base pairs by triplex forming oligonucleotides containing nucleosides derived from 2-aminopyridine.
... respectively (21). Psoralen-linked oligonucleotides containing these modifications have been successfully used to target a portion of the aromatase gene (21). These initial studies with AP-containing oligonucleotides ...
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Development of a Triplex Real-Time PCR Assay for Simultaneous Detection of Allergenic Ingredients in Processed Food
... a triplex Q-PCR method was developed to detect trace amounts of peanut, soybean and sesame in processed ...the triplex PCR was optimised. The triplex method showed high specificity and sensitivity ...
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Efficacy of Filter Cake and Triplex Powders Against Three Internally Developing Stored-product Insect Species
... and Triplex have properties similar to silica-based inert dusts, because of their higher atomic percentage of silicon and oxygen in the form of silicon dioxide ...and Triplex was less than that found in ...
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Asymmetric triplex metallohelices with high and selective activity against cancer cells
... parent triplex compounds showed no signs of toxicity (bactericidal or inhibitory action) towards Methicillin-Resistant Staphylococcus aureus (MRSA - USA300) or Escherichia coli (TOP10) at up to the highest ...
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In vivo evaluation of immunomodulatory properties of crude extracts of Echinacea species and fractions isolated from Echinacea Purpurea
... Echinacea Triplex™, Echinacea purpurea, and Echinacea angustifolia as well as polysaccharide, alkylamide and phenolic fractions prepared from Echinacea purpurea to mice vaccinated with killed Salmonella ...
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Oxidation of DNA by Long-range Charge Transport
... When intercalators were tethered directly to the 5' terminus of the triplex-forming strand as opposed to the center, significant amounts of oxidative damage was generated only in t[r] ...
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SEPT9 and SHOX2 DNA methylation status and its utility in the diagnosis of colonic adenomas and colorectal adenocarcinomas
... SHOX2 harbors two large CpG islands, located at the 3′ and the 5′ end of the gene, and is involved in limb formation and cardiac development [27, 28]. So far, the SHOX2 DNA methylation status has mainly been ...
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Internally Controlled Triplex Quantitative PCR Assay for Human Polyomaviruses JC and BK
... Assay detection limit and reproducibility. The LightCycler 480 was used to assess the detection limit and reproducibility of the triplex qPCR assay. The JCPyV assay had a slightly lower limit of detection than the ...
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Development and Validation of a Multiplex Real Time PCR Assay for Simultaneous Genotyping and Human T Lymphotropic Virus Type 1, 2, and 3 Proviral Load Determination
... Specificity, sensitivity, and dynamic range of qPCR. Figure 1 shows the alignments of the sequences from several HTLV and STLV strains, corresponding to the primer and probe regions. We verified the specificity of the ...
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Influence of Radial Fins and Turbulence Promoters on the Enhancement of Charging and Discharging of Phase Change Heat Transfer in Triplex-Tube
... the triplex tube is mainly used to heat the walls of the tube and, thus to raise the temperature of the tube, which explains the rapid raise of the tube wall temperature during this ...
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Wernike, Kerstin (2012): Establishment of a multiplex real-time PCR assay for the detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1. Dissertation, LMU München: Tierärztliche Fakultät
... a triplex quantitative real-time polymerase chain reaction (qPCR) for testing BoHV-1 was ...The triplex BoHV-1-qPCR has an analytical sensitivity of less than 10 genome copies per reaction, and the ...
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Asymmetric triplex metallohelices with high and selective activity against cancer cells
... however, the high symmetry and modest functionality of current systems offer little scope to tailor the structure to interact with specific biolomolecular targets, or to create libraries for phenotypic screens. Here we ...
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Molecular Interactions of Epstein-Barr Virus Capsid Proteins
... a triplex that is similar to that formed by the minor capsid proteins, VP19C and VP23, of herpes simplex virus type 1 ...EBV triplex interact with the major capsid protein differently from those in ...
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