two-photon fluorescence microscopy
<p>Comparative Two-Photon Fluorescence Microscopy Analysis of Riboflavin Penetration in Two Different Solutions: Dextran and Hydroxypropyl Methylcellulose</p>
... multi- photon microscope (LSM 780 microscope; Zeiss, Oberkochen, Germany) which operates pulsed infrared laser light generating images through the excitation of ...Multiphoton microscopy has the advantage ...
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Towards Heralded Two-Photon Absorption and Two-Photon Excited Fluorescence for Quantum Microscopy and Spectroscopy
... Two-photon microscopy (TPM) is one of the state of the art high resolution imaging technologies used in the life sciences sector [9] exploiting ...of two- photon microscopy are ...
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Apertureless near field optical microscopy for fluorescence imaging
... the two effects. Fluorescence quen- ching could be identified by taking fluorescence lifetime measurements using a pulsed laser and measuring the delay between a laser pulse and the fluorescent ...
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Interaction of Poxvirus Intracellular Mature Virion Proteins with the TPR Domain of Kinesin Light Chain in Live Infected Cells Revealed by Two-Photon-Induced Fluorescence Resonance Energy Transfer Fluorescence Lifetime Imaging Microscopy
... Using two-photon-induced fluorescence lifetime imaging microscopy, we corroborate an interaction (previ- ously demonstrated by yeast two-hybrid domain analysis) of full-length vaccinia ...
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Labeling Human Mesenchymal Stem Cells with Gold Nanocages for in vitro and in vivo Tracking by Two-Photon Microscopy and Photoacoustic Microscopy
... these two modalities are limited in terms of the requirement for genetic modifications of the cells, the cost of radionu- clides with short half-lives, and relatively poor reso- lution ...for fluorescence ...
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A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles
... a two-photon fluorescence lifetime imaging microscopy (FLIM) method monitoring the Foster resonance energy transfer (FRET) effect between gold nanorods (GNRs) particles and fluorescent ...
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Multi-photon excitation microscopy
... excitation microscopy (MPE) has its roots in two-photon excitation (2PE) microscopy whose story dates back more than 70 ...of two-photon quantum transitions in atoms and ...
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Fluorescence excitation analysis by two-photon confocal laser scanning microscopy: a new method to identify fluorescent nanoparticles on histological tissue sections
... using two-photon CLSM (Zeiss LSM 710 NLO; Carl Zeiss, Oberkochen, Germany), equipped with ScanModule (Carl Zeiss), photomultipliers, and nondescanned ...a two-photon CLSM in sequences of ...
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Assessment of membrane fluidity in individual yeast cells by Laurdan generalised polarisation and multi-photon scanning fluorescence microscopy
... The GP may theoretically range from –1 to +1. This GP parameter is inversely related to membrane fluidity; high GP values are found in gel phase, and low in liquid-crystalline phase [15]. Laurdan has been used to ...
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Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... trol (Figure 4, panels A1-3) and next on cells co-expressing Vpr-eGFP and mCherry fusion proteins (Figure 4, panels B1-3 and C1-3). An arbitrary color scale, ranging from blue to red, illustrates short to long lifetimes. ...
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Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... confocal microscopy which has good spatial and temporal resolution and which also has the power to detect individual molecular species in living cells based on fluorescence or Raman spectroscopy ...[4,5]. ...
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Super resolution Imaging by Two photon Structured Illumination Microscopy
... conventional fluorescence microscopy is limited by the optical ...super-resolution fluorescence microscopy techniques about breaking the diffraction limit can be divided into two groups ...
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Recent advances in hydrogen peroxide imaging for biological applications
... confocal microscopy [28]. In the two-photon process, a molecule simultaneously absorbs two photons whose individual en- ergy is only half of the energy needed to excite that mol- ecule, and ...
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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre
... ultrashort pulses can propagate through PCF but in their investigation the emitted pulses are stretched from 100 fs to 140 fs 6 . We report on a system which compresses the pulse duration and, using a conventional ...
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Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... steady-state fluorescence intensity still suffered from artifacts arising from the heterogeneity of tissue optical properties [8], local environment, unknown chromophores with over- lapped spectra, and the ...
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Three photon light sheet fluorescence microscopy
... (PI). Fluorescence was collected perpendicularly to the illumination plane by a second objective lens (Olympus, ...40x. Two fluores- cence filters (FF01-680/SP, FF01-468/SP, Semrock) were used to block ...
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Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
... the fluorescence detected over a period of 100 s of continuous excitation of a single ND using 1 mW of average power at 532 ...in fluorescence of 50% in samples labelled with Alexa 488 after 100 frames of ...
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Light sheet fluorescence microscopy for neuroscience
... acquisition of volumetric images very long. The wide light exposure combined with the limited acquisition speed results in a much higher exposure of the sam- ple causing potential photobleaching. Moreover, high ...
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Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
... Light microscopy data showed the absence of tissular damages in lungs of control rats. Moreover, the exposure to SMFs failed to alter lungs architecture in rats. How- ever, GNPs treatment induced mild epithelial ...
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Smear microscopy and culture conversion rates among smear positive pulmonary tuberculosis patients by HIV status in Dar es Salaam, Tanzania
... sputum microscopy, after two weeks of treatment the conversion rate was higher in HIV positive ...smear microscopy during the rest of the treatment period (8, 12 and 20 weeks) were not signifi- ...
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