Type I membrane fusion
The Transmembrane Domain of the Respiratory Syncytial Virus F Protein Is an Orientation-Independent Apical Plasma Membrane Sorting Sequence
... cell membrane (29). The fusion (F) protein of RSV is a type I single-pass integral mem- brane protein embedded in the cell membrane of infected cells and in the viral envelope after ...
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Role of the Specific Amino Acid Sequence of the Membrane-Spanning Domain of Human Immunodeficiency Virus Type 1 in Membrane Fusion
... the membrane-spanning domain (MSD) has been rather ...in membrane fusion as well as other gp41 functions is not well ...of membrane spanning ␣ ...in membrane fusion, by making ...
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Binding-Site Interactions between Epstein-Barr Virus Fusion Proteins gp42 and gH/gL Reveal a Peptide That Inhibits both Epithelial and B-Cell Membrane Fusion
... for membrane fusion, which is different from other known viral fusion systems that utilize a single fusion protein ...rus type 1 (HSV-1) functional homolog gD bound to the her- pesvirus ...
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Herpes Simplex Virus Type 1 Entry Is Inhibited by the Cobalt Chelate Complex CTC-96
... if it enters cells. However, our results suggest that these are not alternative targets of CTC-96. It seems likely that CTC-96 does not enter cells efficiently because of the temporal requirement for addition of CTC-96 ...
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Examination of a Fusogenic Hexameric Core from Human Metapneumovirus and Identification of a Potent Synthetic Peptide Inhibitor from the Heptad Repeat 1 Region
... its fusion (F) protein to other well-characterized viruses utilizing type I integral membrane proteins as fusion ...significant fusion inhibitor with a 50% inhibitory ...
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Mapping and Characterization of the N-Terminal I Domain of Human Immunodeficiency Virus Type 1 Pr55Gag
... an I domain are found in distinct foci underlying the plasma membrane, while Gag-GFP constructs lacking the I domain are distributed more diffusely within the cellular cytoplasm ...the I ...
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Importance of the Membrane-Perturbing Properties of the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 gp41 to Viral Fusion
... of fusion, but all of these are based on studies with peptides corresponding to the MPER and are limited by the lack of a structure for the ectodomain of the native Env ...mutant I-AA or I-ITN ...
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Role of the Membrane-Proximal Domain in the Initial Stages of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein-Mediated Membrane Fusion
... the membrane with DiI and in the cytosol with calcein as previously described ...and I) and fluorescence microscopy for CMAC staining (B, F, and J), for DiI staining (C, G, and K), and for calcein staining ...
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The Pre-Transmembrane Domain of the Autographa californica Multicapsid Nucleopolyhedrovirus GP64 Protein Is Critical for Membrane Fusion and Virus Infectivity
... virus type 1 and Epstein- Barr virus) gB glycoproteins and also to the structures of the vesicular stomatitis virus (VSV) G protein (1, ...internal fusion loops that are likely associated with target ...
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Restoration of Function of Carboxy-Terminally Truncated Pseudorabies Virus Glycoprotein B by Point Mutations in the Ectodomain
... virus type 1 (HSV-1) glycoprotein C (gC) (8, ...i.e., fusion of the viral envelope and the cel- lular plasma membrane at neutral pH, glycoproteins gD and gB and the gH-gL complex are required (21, ...
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Functional Analysis of the Autographa californica Multiple Nucleopolyhedrovirus GP64 Terminal Fusion Loops and Interactions with Membranes
... endosome membrane, and nucleocapsids (NC) are released into the cytoplasm where they are subsequently trans- ported to the ...nucleus. Fusion of the viral envelope with the endo- some membrane is ...
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1 Mitochondrial dynamics in stem cells and
... mitochondrial outer membrane (MOM) fusion, mediated by mitofusin proteins (Mfn1 and Mfn2) 48.. [13-17] and mitochondrial inner membrane (MIM) fusion, mediated by Opa1, and could be poss[r] ...
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Structural and Functional Characterization of Membrane Fusion Inhibitors with Extremely Potent Activity against Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus
... only membrane fusion inhibitor available for the treatment of viral infection; however, it has low anti-human immunodeficiency virus (anti-HIV) activity and a low genetic barrier for drug ...
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DETECTION OF KLEBSIELLA PNEUMONIAE FROM CLINICAL AND MEDICAL WASTE SAMPLES USING EFFLUX GENES (RAMA AND ACRA)
... (Outer membrane protein changes and efflux pump expression together may confer resistance to ertapenem in Enterobacter cloacae Antimicrob Agents Chemother; 2006; 50: ...
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Multifaceted Sequence-Dependent and -Independent Roles for Reovirus FAST Protein Cytoplasmic Tails in Fusion Pore Formation and Syncytiogenesis
... affect membrane fusion independent of effects on cell surface protein ...the fusion pheno- types, plasmid DNA transfection conditions were modified to normalize FAST protein expression in the plasma ...
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Fusion properties of cells persistently infected with human parainfluenza virus type 3: participation of hemagglutinin-neuraminidase in membrane fusion.
... Experiments were performed to determine whether the presence of neuraminic acid residues on the surface of the uninfected cells was necessary for fusion to occur when these cells were ad[r] ...
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Herpes Simplex Virus Type 1 gK Is Required for gB-Mediated Virus-Induced Cell Fusion, While neither gB and gK nor gB and UL20p Function Redundantly in Virion De-Envelopment
... H, I, J, K, and L) recombinant viruses containing specific gB mutations on Vero (A, C, E, G, I, and K) and FcgK-1 (B, D, and F), or Fd20-1 (H, J, and L) ...
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Membrane Anchoring of Epstein Barr Virus gp42 Inhibits Fusion with B Cells Even with Increased Flexibility Allowed by Engineered Spacers
... whether membrane-bound gp42 could be made functional by increasing separation from the membrane, the nat- urally occurring structured immunoglobulin-like domain I27 from the multidomain muscle protein titin ...
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Production and Characterization of a Soluble, Active Form of Tva, the Subgroup A Avian Sarcoma and Leukosis Virus Receptor
... virus type 1 (HIV-1) envelope results in dissociation of gp120 from gp41 (47), acquisition of protease sensitivity by HIV-1 Env (56), and alterations in monoclonal antibody binding to HIV-1 Env (31, 41, 56) and ...
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Truncation of the Membrane-Spanning Domain of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Defines Elements Required for Fusion, Incorporation, and Infectivity
... Previous C-terminal-truncation studies of simian immuno- deficiency virus (SIV) Env (19, 41) suggested that the entire 27-amino-acid region is not required for the biological function of the protein. In the case of SIV, ...
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