Type V

Top PDF Type V:

McArdle’s Disease (Glycogen Storage Disease type V): A Clinical Case

McArdle’s Disease (Glycogen Storage Disease type V): A Clinical Case

A 35 years old woman was irst diagnosed in a Neurology’s consultation at the age of 21, with a delayed diagnosis of at least 10 years. The clinical signs included: intolerance to exercise, asthenia, muscular weakness and episodes of rhabdomyolysis. The diagnosis was established by a muscular biopsy, showing vacuolar myopathy with negative myophosphorylase linked to McArdle’s disease or glycogen storage disease type V (Figures 1,2). The genetic study was performed 12 years after the clinical diagnosis: A homozygotic mutation in the PYGM gene was found, pathogenic variant c.C148T:p.R50X.
Show more

5 Read more

Anti-collagen type v: a marker of early systemic sclerosis?

Anti-collagen type v: a marker of early systemic sclerosis?

Anti-Collagen type V was performed by ELISA. Briefly, wells of polystyrene microplates (Immulon 1 B plaque) were sensitized overnight with 200 μL of purified human collagen V (Sigma) (1 g/ml) and then blocked with BSA 1% (Sigma) for 1 h and 30 min inside a humid chamber at room temperature. The diluted 1:50 serum samples were added to the wells and tested in duplicates. The conjugate used was the anti-human IgG Sigma (A8542) at 1/1000 dilution. The reaction was revealed after add- ing a solution of disodium phosphate 20 mg Sigma P-nitrophenylphosphate (N2765) in 20 mL of glycine buffer. The optical density (OD) was read at 405ηm (ELISA Multiskan MS, Labsystems, Helsinki, Finland). Positive results were defined as OD = 3 SD above the mean OD of controls sera samples.
Show more

5 Read more

Type V collagen induces apoptosis of 8701 BC breast cancer cells and enhances m calpain expression

Type V collagen induces apoptosis of 8701 BC breast cancer cells and enhances m calpain expression

decrease of the expected growth rate in a given cell population may be due to two possible responses: a slow-down of the duplication rates or an increase rate of programmed cell death. The data presented here clearly support the second hypothesis. As it is known, apoptosis is a cell death program that produces specific morphological and biochemical modification, including loss of adherence to the substrate, cell shrinking in volume and chromatin condensation and separation into discrete masses, that produce a ladder-like pattern when the fragmented DNA is electrophoresed through agarose gels [60]. All the above-mentioned phenomenoma have been shown to occur in a large percentage of cells cultured onto type V collagen substrate.
Show more

20 Read more

Type V Protein Secretion: Simplicity Gone Awry?

Type V Protein Secretion: Simplicity Gone Awry?

the 106-kDa IgA1 protease and a small stable α -protein. In this model, neither accessory factors for transit from the periplasm to the bacterial surface nor external energy for the secretion across the outer membrane are required. Therefore, this secretion system and the proteins secreted via this pathway have been designated autotransporters. Secreted proteins belonging to the type V secretion family (a) contain all the information for translocation through the cell envelope and/or require a single accessory factor and (b) translocate across the outer membrane via a transmembrane pore formed by a β -barrel structure (Henderson et al., 1998; Thanassi and Hultgren, 2000). The proteins secreted by this pathway possess unifying characteristics including: (i) an N-terminal signal sequence for inner membrane translocation, (ii) a functional passenger domain that can be surface-exposed or released into the extracellular milieu, (iii) a linker region necessary for translocation of the passenger domain through the outer membrane, (iv) a C-terminal region involved in the formation of a transmembrane pore. Based on these and others criteria, the two-partner system (TPS) has been grouped under the type V secretion umbrella as a distinct subgroup (Henderson et al., 2000a,b; Jacob-Dubuisson et al., 2000). In contrast to the autotransporter pathway where the protein is produced as a single polypeptide, in the TPS the passenger domain (also called the exoprotein) and the pore forming β-domain (also called transporter domain) are translated as two separate proteins, respectively referred as TpsA and TpsB family members (Jacob-Dubuisson et al., 2001). From an evolution point (Danchin, 2003), it is still diffi cult to discriminate between divergent evolution from a common ancestor or convergent evolution towards a similar solution for secretion of large proteins across the outer membrane (Henderson et al., 2000a,b; Jacob-Dubuisson et al., 2000). Nevertheless, it appears from homology comparisons and the similarities in the gross anatomy of the autotransporter and TPS secretion pathways that the two pathways are related. Thus, in a manner similar to the type IV secretion pathway (Christie and Vogel, 2000), it has been proposed to classify autotransporter protein secretion as type V a secretion and the TPS in the subgroup of type V b
Show more

15 Read more

Increased prevalence of apolipoprotein E4 in type V hyperlipoproteinemia

Increased prevalence of apolipoprotein E4 in type V hyperlipoproteinemia

triglyceride-rich lipoproteins, has been implicated in the receptor-mediated hepatic uptake of these particles. ApoE has three major alleles: E2, E3, and E4, and the products of these alleles are apoE2, apoE3, and apoE4, respectively. ApoE phenotypes were determined in 30 type V HLP patients as well as in 37 normal volunteers. Among the type V patients, 33.3% were noted to be homozygous, and 40.0% heterozygous for E4 (normal, 2.7 and 21.6%, respectively). These data suggest that apoE4 may play a role in the etiology of the hyperlipidemia in a significant number of type V HLP patients.
Show more

5 Read more

Higher dimensional bianchi type V universe in creation field cosmology

Higher dimensional bianchi type V universe in creation field cosmology

In this paper, we have considered the space-time geome- try corresponding to Bianchi type-V in Hoyle-Narlikar [15-17] creation field theory of gravitation with five di- mensions. Bianchi type-V universe in creation-field cos- mology has been investigated by Singh and Chaubey [19] whose work has been extended and studied in five di- mensions. An attempt has been made to retain Singh and Chaubey’s [19] forms of the various quantities. We have noted that all the results of Singh and Chaubey [19] can be obtained from our results by assigning appropriate values to the functions concerned.
Show more

5 Read more

Special type V through solar water heater

Special type V through solar water heater

a brass sheet with a dimension of 14 cm (width) ×100 cm (length) × 0.5mm (thickness) to two equally distant copper pipes with outer diameter of 12.5 cm each. Although the width of the constructed absorber is 0.5 cm different from that of width applied in the optical analysis, the small discrepancy in the collector width does not incur any influence to the final result as it can be explained in the following two reasons. First, the ratio of V-trough aperture size per absorberarea still remains the same in both cases. Second, the average solar concentration throughout the whole area of absorber is taken in account. It is inevitable to consider the absorber dimension of14 cm × 100 cm in the optical analysis owing to the convenience to represent it by the equivalent 141×1001 reflective pointscompared to the dimension of 14
Show more

9 Read more

The Clinical, Biochemical, and Familial Presentation of Type V Hyperlipoproteinemia in Childhood

The Clinical, Biochemical, and Familial Presentation of Type V Hyperlipoproteinemia in Childhood

and abnormal glucose tolerance tests in children and adolescents. Lestradet 11, 1)escliamps I, Giron B: Insulin and free fatty acid levels during oral glucose tolerance tests and their r[r]

15 Read more

Role of the Cytoplasmic Domain of the β-Subunit of Integrin αvβ6 in Infection by Foot-and-Mouth Disease Virus

Role of the Cytoplasmic Domain of the β-Subunit of Integrin αvβ6 in Infection by Foot-and-Mouth Disease Virus

transduction through ␣v␤6 could be required to activate a coreceptor necessary for virus internalization. Signal transduc- tion through integrins is known to regulate the ligand-binding affinity of other integrin species expressed on the same cell by a mechanism termed integrin cross-talk (7). SW480 cells nor- mally express two RGD-dependent integrins, ␣v␤5 and ␣5␤1, and either of these integrins could conceivably be activated following virus binding to ␣v␤6. However, as we showed pre- viously that neither of these integrins appear to be involved in infection of SW480 cells expressing wild-typev␤6, this mech- anism may be discounted (31). Although we consider it un- likely, we cannot rule out the possibility that signaling through the ␤6 cytoplasmic domain is necessary for activation of a nonintegrin coreceptor. Alternatively, virus binding to ␣v␤6 could initiate intracellular signaling pathways that are advan- tageous for a postentry step in virus replication and/or assem- bly. However, since mock-transfected SW480 cells in the ab- sence of ␣v␤6 are permissive for a heparin-binding strain of FMDV (31), such a role for ␣v␤6 in infection would similarly appear unlikely. However, on the basis of our data, we cannot rule out the possibility that signaling through ␣v␤6 may be required to promote infection by FMDV by stimulating endo- cytosis of ␣v␤6 itself. For example, endosomal uptake of the integrin ␣v␤5 has been shown to be stimulated by ligand bind- ing (37), and more recently, ␣v␤5-mediated endocytosis of adenovirus has been shown to require activation of phospho- inositide-3-OH kinase (PI3K), an event dependent on the ␤5 cytoplasmic domain (33). Using one-step growth curve analy- sis, we have observed that treatment of cells expressing wild- typev␤6 with wortmannin (50 to 200 nM), a specific inhibitor of PI3K, did not affect virus replication, suggesting that PI3K activity is not required for ␣v␤6-dependent infection by FMDV (data not shown).
Show more

7 Read more

Functional outcome of communited intra articular distal radius fractures managed by fragment specific fracture fixation: A Prospective study.

Functional outcome of communited intra articular distal radius fractures managed by fragment specific fracture fixation: A Prospective study.

Type IV: radiocarpal joint involved with ulnar styloid fracture Type V: distal radioulnar joint involved Type VI: distal radioulnar joint involved with ulnar styloid fracture Type VII: b[r]

101 Read more

Vol 8, No 1 (2017)

Vol 8, No 1 (2017)

Magnetic Field. Katore, S.D., Shaikh, A.Y. [22] studied Bianchi Type-V Magnetized Anisotropic Dark Energy Models with constant Deceleration Parameter. Mishra, B.; Mohanty, G. [23] have investigated Bianchi Type-V Cosmological Model in Scale Invarient Theory. Mohd. Zeyauddin; Saha, Bijan [24] have investigated Bianchi type-V bulk viscous cosmological models with particle creation in general relativity. Deo S. D. and Roughe A K [25] have studied Non Existence of Bianchi type I, V and VI 0 cosmic string in Bimetric relativity.

5 Read more

Minimising road infrastructure maintenance costs by managing the traffic

Minimising road infrastructure maintenance costs by managing the traffic

Constants Capacity of road r Flow/Intensity on road section r Growth factor of vehicle type v Number of vehicles of type v on road r State of the pavement of road r on time t Road sectio[r]

97 Read more

Behavior of bulk viscous Bianchi v cosmological model in f(r) theory of gravity

Behavior of bulk viscous Bianchi v cosmological model in f(r) theory of gravity

f gravity have studied by Kiran et al. (2013). Ghate et al. (2014) studied Bianchi type-IX viscous string cosmological model in f ( R , T ) gravity with the special form of declaration parameter. Reddy et al. (2013) have discussed Kaluza-Klein universe with cosmic strings and bulk viscosity in f ( R , T ) gravity. Mahanta et al. (2014) have investigated bulk viscous cosmological model in f ( R , T ) gravity. Reddy et al. (Reddy et al., 2014) have investigated Kantowski-Sachs bulk viscous string cosmological model in f ( R , T ) gravity. Same author (Reddy et al., 2013) have studied LRS Bianchi type-II universe with cosmic strings and bulk viscosity in a modified theory of gravity. Naidu et al. (2013) have studied Bianchi type-V bulk viscous string cosmological model in
Show more

7 Read more

Authenticated  Key  Exchange  from  Ideal  Lattices

Authenticated Key Exchange from Ideal Lattices

Type II , Type III , and Type V adversaries by using the assumption that H 1 is a random oracle. Then, if RLWE q,α is hard, the proposed AKE is secure against any PPT Type I adversary A [r]

38 Read more

A primary cation transport by a V type ATPase of low specificity

A primary cation transport by a V type ATPase of low specificity

experiments) and Fig. 4B (showing a representative recording from four experiments). Depletion of ATP caused the membrane resistance to increase by about 160 % (from 20 to 53 M V in Fig. 4A and from 24 to 65 M V in Fig. 4B). Both inhibitors produced a slight increase in the resistance (30 % for harmaline and 10 % for amiloride) and a slight decrease in the voltage, which were only partly reversible. This combination suggests some inhibition of the primary transport rather than an effect on an antiport. This inference is corroborated by the finding that harmaline or amiloride had no effect on the membrane resistance in the ATP-depleted state (compare Fig. 5A, which is representative of 13 recordings, and Fig. 5B, which is representative of four recordings, with Fig. 4).
Show more

8 Read more

On improving Service Chains Survivability Through Efficient Backup Provisioning

On improving Service Chains Survivability Through Efficient Backup Provisioning

As an example of how shared backups could be placed, we can see in Fig. 1 that physical node 9 hosts a backup of VNF type 3 (i.e., NF3) that is shared between the VNF type 3 of chain 1 and that of chain 2. If physical node 11 fails, and hence NF3 of chain 1 becomes out of service, the backup NF3 hosted in 9 takes over and replace the failed function. Similarly, it can take over the service of NF3 belonging to chain 2 (hosted in node 10) if it fails. The figure also shows other examples of shared VNF backups (e.g., NF1 and NF2 hosted in node 8). It is easy to check that the two service chains shown in this example are perfectly survivable to any single node failure.
Show more

8 Read more

V-TYPE POSITION EFFECTS AT THE LIGHT LOCUS IN DROSOPHILA MELANOGASTER

V-TYPE POSITION EFFECTS AT THE LIGHT LOCUS IN DROSOPHILA MELANOGASTER

uirilis found that the rearrangements which cause pe variegation had, in the main, one break in the proximal heterochromatin of chromosome 5 and another break in only the[r]

9 Read more

2300 5000 01 Advanced Microprocessor Development System Reference Manual Dec78 pdf

2300 5000 01 Advanced Microprocessor Development System Reference Manual Dec78 pdf

Input data is stored in AMOS memory beginning at the memory pointer address of the active side of the display... v[type] [ ,length] type is as defined above.[r]

136 Read more

Scanning Tunneling Spectroscopic Studies on High-Temperature Superconductors and Dirac Materials

Scanning Tunneling Spectroscopic Studies on High-Temperature Superconductors and Dirac Materials

detailed in Chapter 2, �𝑑𝐼 𝑑𝑉 � � �𝐼 𝑉 � � � vs. 𝑉 , from different region with differing lattice arrangements. The spectra for the more honeycombed (relaxed) “ α ” region of the graphene sample resembled the spectra found in mechanically exfoliated graphene and are Dirac-like (V-shaped) except a substantially larger zero bias conductance and an addition of several weak conductance peaks. In contrast, the typical spectra associated with the square lattice (strongly strained) “ β ” region appeared to be asymmetric relative to those in the “ α ” region, with sharp conductance peaks more closely spaced. For “ α ” and “ β ” regions and the representative spectra found in each region, the energy interval between consecutive conductance peaks appears to decrease with increasing energy. For disordered, amorphous “ γ ” regions without atomic resolution, almost completely parabolic spectra that deviates fundamentally from the linear energy dependence of the Dirac fermions was found. While the “ γ ” region spectra does also demonstrate conductance peaks, the conductance peaks seem randomly spaced. Figure 6.10 shows topography images of each region type and the representative spectra for each region. Figure 6.11 shows similar results achieved and verified on a second graphene sample prepared exactly as the first. The spatial variations in the spectra (tunneling conductance) depends very sensitively on the local strain of the lattice.
Show more

191 Read more

The mass of the white dwarf in the old nova BT Mon

The mass of the white dwarf in the old nova BT Mon

. From these parameters we obtain a mass of 1 : 04 6 0 : 06 M ( for the white dwarf primary star and a mass of 0 : 87 6 0 : 06 M ( for the G8 V secondary star. The inclination of the system is found to be 82 8 : 2 6 3 8 : 2 and we estimate that the system lies at a distance of 1700 6 300 pc. The high mass of the white dwarf and our finding that BT Mon was probably a fast nova together constitute a new piece of evidence in favour of the thermonuclear runaway model of classical nova outbursts. The emission lines are single- peaked throughout the orbital cycle, showing absorption around phase 0.5, high-velocity S-wave components and large phase offsets in their radial velocity curves. In each of these respects, BT Mon is similar to the SW Sex stars. We also find quasi-periodic flaring in the trailed spectra, which makes BT Mon a candidate intermediate polar.
Show more

18 Read more

Show all 10000 documents...