UPLC/Q-TOF-MS
VALIDATED UPLC/Q TOF MS METHOD FOR SIMULTANEOUS DETERMINATION OF ACECLOFENAC AND PARACETAMOL IN HUMAN PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY
... of UPLC/Q-TOF-MS ...time. Q-TOF mass spectrometry gives accurate mass measurement and reliable chemical fragmentation, which are ultimately helpful in structure elucidations of ...
11
Tissue-specific chemical profiling and quantitative analysis of bioactive components of Cinnamomum cassia by combining laser-microdissection with UPLC-Q/TOF–MS
... Tissue-specific chemical profiles were obtained as base peak chromatograms by UPLC-Q/TOF–MS (repre- sentative chromatograms are showed in Fig. 3). A total of 19 peaks were unequivocally or ...
9
VALIDATED UPLC/Q TOF MS METHOD FOR STRUCTURAL IDENTIFICATION AND QUANTITATIVE DETERMINATION OF ANTIHYPERTENSIVE DRUGS
... an UPLC/Q-TOF-MS method is developed and validated for structural identification and quantitative determination of five antihypertensive drugs that are, Losartan, Telmisartan, Valsartan, ...
8
Anti aging potential of extracts from Sclerocarya birrea (A Rich ) Hochst and its chemical profiling by UPLC Q TOF MS
... Background: Degradation of components of the extracellular matrix such as elastin and collagen by elastase and collagenase accelerates skin aging. Phytochemicals that inhibit the activity of these enzymes can be ...
14
Metabolomics profiling of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata extracts using UPLC-Q/TOF-MS
... injury; UPLC-Q/TOF- MS: ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry; MS: mass spectrometry; ALT: alanine aminotransferase; AST: aspartate ...
17
68. Validated UPLC-Q-TOF-MS method for quantitative determination of emodin in rhizome of Rheum emodi Wall. ex Meissn. and its traditional polyherbal formulation using three different extraction techniques
... In this paper for the first time, a rapid, sensitive and high throughput ultra-performance liquid chromatography/quadrupole time-of-flight mass spectroscopy (UPLC/Q-TOF-MS) method was ...
6
Validated UPLC/Q TOF MS Method for Determination of Poliumoside in Rat Plasma and Its Application to Pharmacokinetic Study
... Poliumoside is the main active constituent of Callicarpa kwangtungensis Chun (CK), a traditional Chinese medicine for management of hemostasis. In this study, a rapid and selective ultra-per- formance liquid ...
9
Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS
... cryphtotanshinone (CTan) and salvianolic acid B (SaB) [7–9]. Mapping the distribution of these bioactive com- ponents and carrying out semi-quantitative analysis in various herbal tissues can help to evaluate pharmaceu- ...
13
Quantification of Tamoxifen Polymeric Nanoparticles in female rodent breast tissue by UPLC/ESI-Q-TOF MS/MS
... on UPLC-ESI-Q-TOF-MS/MS has been developed and validated for the quantitative determination of TMX in female mice plasma using Clomi- phine as an Internal Standard ...ACQUITY ...
9
Changes of myocardial lipidomics profiling in a rat model of diabetic cardiomyopathy using UPLC/Q-TOF/MS analysis
... An ultra-high-performance liquid chromatography (UPLC) coupled to quadruple time-of flight (QTOF) mass spectrometer (Waters MS Technologies, Manches- ter, UK) was used for myocardial tissue sample analysis. ...
9
<p>UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression</p>
... In recent years, metabonomics – a quantitative determina- tion of the dynamic metabolic response of biological system to pathophysiological stimulation or genetic modi fi cation – has been widely used. 11 Powerful ...
12
Assessing the quality of Smilacis Glabrae Rhizoma (Tufuling) by colormetrics and UPLC-Q-TOF-MS
... the UPLC system was set at 2 µL. The ESI source of the MS was connected to the UPLC system via a capillary through the UV cell ...outlet. MS data were collected with a capillary voltage of ...
9
Lipidomics coupled with pathway analysis characterizes serum metabolic changes in response to potassium oxonate induced hyperuricemic rats
... Methods: Lipidomics-a specialized study of lipid metabolites-has become a highly sensitive and powerful tool for biomarker discovery. In this work, an ultra-performance liquid chromatography-quadruole-time-of-flight ...
10
Calcium-deficiency assessment and biomarker identification by an integrated urinary metabonomics analysis
... the UPLC/Q-TOF-MS data, the supervised partial least-squares discriminant analysis (PLS-DA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA) were applied to ...
18
Simultaneous UPLC–TQ-MS/MS determination of six active components in rat plasma: application in the pharmacokinetic study of Cyclocarya paliurus leaves
... using UPLC-Q-TOF/MS; 13 absorbed components were identified—quinic acid, gallic acid, neochlorogenic acid, chlorogenic acid, p-hydroxybenzoid acid, quercetin-3-O-glucuronide, ...
11
Combination of 15 lipid metabolites and motilin to diagnose spleen-deficiency FD
... were uncertain causal relationships between the bio- markers and the occurrence of spleen deficiency syn- drome. Although the combination of metabonomics data and clinical features did not show a good predictive func- ...
14
Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi
... MALDI-TOF/TOF MS (Bruker Daltonik GmbH, Bremen, ...for MS measurements and between 60 and 250 ppm for tandem MS (MS/MS) measurements were ...all MS and ...
7
Mass spectrometrical analysis of recombinant human growth hormone (Genotropin®) reveals amino acid substitutions in 2% of the expressed protein
... Deamidation of the amino acids N 149 and N 152 was also detected. The molecular mass of the peptide RLEDGSPR was decreased with 28 Da. The mass difference could be localized to the N terminus of the peptide and might ...
12
Investigation of phytochemicals in methanolic leaves extracts of Abutilon pannosum and Grewia tenax by Q-TOF LC/MS
... of TOF analysis in both the MS and tandem MS (MS/MS) modes, with the recognized and broadly utilized systems of electrospray ionization (ESI) and atmospheric pressure chemical ...
7
Simultaneous determination of five azadirachtins in the seed and leaf extracts of Azadirachta indica by automated online solid-phase extraction coupled with LC–Q-TOF–MS
... A fully automated online SPE-LC–Q-TOF–MS method was developed for the simultaneous determination of five azadirachtins in the seed and leaf extracts of A. indica. The online SPE-LC system was able to ...
9