Viral Protein R
Efficient DNA Transfection Mediated by the C-Terminal Domain of Human Immunodeficiency Virus Type 1 Viral Protein R
... Viral protein R (Vpr) of human immunodeficiency virus type 1 is produced late in the virus life cycle and is assembled into the virion through binding to the Gag ...the viral life cycle by ...
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Modulation of NKG2D-Mediated Cytotoxic Functions of Natural Killer Cells by Viral Protein R from HIV-1 Primary Isolates
... Viral protein R from HIV-1 primary isolates and laboratory-adapted virus. Nine primary vpr alleles spanning four HIV-1 groups (M, N, O, and P) were selected. For pandemic group M, two alleles were ...
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The effect of childhood trauma, ApoE genotype and HIV 1 viral protein R variants on change in cognitive performance
... Objective: Gene–environment interactions contribute to the development of HIV-associated neurocognitive disorders. We examined whether childhood trauma, apolipoprotein E isoforms and viral protein R ...
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Development of a robust cell-based high-throughput screening assay to identify targets of HIV-1 viral protein R dimerization
... Targeting protein–protein interactions (PPI) is an emerging field in drug ...HIV-1 viral protein R (Vpr) dimerization as a “proof of ...
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Production of Uninfectious Human Immunodeficiency Virus Type 1 Containing Viral Protein R Fused to a Single-Chain Antibody against Viral Integrase
... Viral protein R (Vpr) of human immunodeficiency virus type 1 (HIV-1), a virion-associated accessory protein consisting of approximately 100 amino acids (11 to 16 kDa), is as abun- dant as Gag ...
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Astrocytic expression of HIV-1 viral protein R in the hippocampus causes chromatolysis, synaptic loss and memory impairment
... Astrocyte expression of HIV-1 Vpr in the hippocampus induces structural changes indicating damage to neurons Astrocytes respond to viral neurotoxins by releasing pro- inflammatory molecules, which attract ...
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Cell cycle G2/M arrest through an S phase dependent mechanism by HIV 1 viral protein R
... Cdc25A protein degradation, we next examined whether Cdc25B/C are the substrates of Chk1 for Vpr to induce G2 ...Cdc25B/C protein levels were com- pared by Western blot analyses using anti-Cdc25B or ...
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Viral protein R of human immunodeficiency virus type 1 induces retrotransposition of long interspersed element 1
... signal intensity of the 140 bp band, which corresponds to a product of L1-RTP, was increased by treatment with rVpr (Figure 1B, lower panel for the rationale of the PCR-based assay and 1D, lane 2). A quantitative PCR ...
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Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions.
... Cells were labeled with [3H]leucine, cell supernatants were harvested, and disrupted cells were fractionated into membrane, membrane wash, cytosolic, and postnuclear wash fractions and v[r] ...
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Cellular phenotype impacts human immunodeficiency virus type 1 viral protein R subcellular localization
... the viral life cycle at the single-cell level and/or overall host pathogenesis and disease (reviewed in ...ferent protein tags primarily in readily transfectable eukar- yotic cell lines (summarized in Table ...
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Structural alteration of DNA induced by viral protein R of HIV 1 triggers the DNA damage response
... of R-loops (DNA/RNA hybrids), which are formed when Topo1 inhibition blocks the transcrip- tion [59, ...that R-loop formation is a major trigger event of Vpr-induced ...in R-loops at the ...
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Nonstructural protein Pns4 of rice dwarf virus is essential for viral infection in its insect vector
... of viral infection and it formed bundles of minitubules at later stages of viral infection in cultured insect vector cells ...and viral rep- lication were strongly inhibited both in cultured insect ...
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Rotavirus Nonstructural Protein NSP3 Is Not Required for Viral Protein Synthesis
... in protein synthesis and therefore an important target for ...poly(A)-binding protein (PABP). eIF4G is a scaffold protein that brings together eIF4E and PABP, causing the circularization of the mRNA ...
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Flavivirus NS1: a multifaceted enigmatic viral protein
... Flaviviruses are emerging arthropod-borne viruses representing an immense global health problem. The prominent viruses of this group include dengue virus, yellow fever virus, Japanese encephalitis virus, West Nile virus ...
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Diagnostic accuracy of transient elastography for early detection of hepatoceelular carcinoma in viral etiology related liver cirrhosis
... Ultrasound abdomen and Hepatitis B and C serology by ELISA were done for all patients on the same day. Transient Elastography was performed by FibroScan® (502 TOUCH; E300M001.2, version 2) in all the patients for the ...
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Antiapoptotic Activity of the Cardiovirus Leader Protein, a Viral “Security” Protein
... the viral antiapoptotic function(s) is suppressed, as is the case in infection with L-deficient mutants (another MV mutant with a nearly complete deletion of L protein exhibited apoptosis-inducing activity ...
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Expression of the Vaccinia Virus Antiapoptotic F1 Protein Is Blocked by Protein Kinase R in the Absence of the Viral E3 Protein
... halts viral replication and limits the spread of ...such viral Bcl-2-like antiapoptotic protein is vaccinia virus F1, which localizes to the outer mitochondrial membrane and protects the infected ...
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The Herpes Simplex Virus Virion Host Shutoff Protein Enhances Translation of Viral True Late mRNAs Independently of Suppressing Protein Kinase R and Stress Granule Formation
... and viral mRNAs (reviewed in reference ...the viral ICP34.5 protein binds protein phosphatase 1␣, redirect- ing it to dephosphorylate eIF2 ␣ (35), the US11 protein binds dsRNA and PKR ...
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Regulation of herpesvirus macromolecular synthesis. VI. Synthesis and modification of viral polypeptides in enucleated cells.
... To examine the function of the nucleus in the modification of viral proteins, in the decline of a and host protein synthesis and in the induction of /3 and y viral polypeptide synthesis,[r] ...
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Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription.
... Lanes: A and F, controls showing the virus-specific RNA extracted from infected cells; D, all seven virus-specified RNAs immunoprecipitated by the anti-N monoclonal antibody; B, C, and E[r] ...
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