Viral RNA extraction from stool samples
Comparison of RNA extraction methods from biofilm samples of Staphylococcus epidermidis
... mRNA from biofilms is crucial to validate the transcriptional measurements associated with the switching to the biofilm mode of ...available RNA extraction kits with distinct characteristics, ...
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Shiga Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada
... is viral or bacterial, as enteric viral infections are more frequently found during the winter months, whereas enteric bacterial infections are more prevalent from May to ...and viral ...
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Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization
... Analysis of fecal specimens from an experimentally infected marmoset and an epidemic of hepatitis A showed that HAV excretion could often be detected later in the illness by hybridizatio[r] ...
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Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
... DNA from specimens for molecular diagnostics, since fecal samples also contain DNA from human cells and many different substances derived from food, cell residues and medication that can ...
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Prevalence, Types, and RNA Concentrations of Human Parechoviruses, Including a Sixth Parechovirus Type, in Stool Samples from Patients with Acute Enteritis
... patients from outbreak and hospital settings, no HPeV was ...detected from 39 control patients. Positive samples occurred only in summer and ...of viral loads was 3,170 to 503,377,290 copies ...
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Detection of Human Parechoviruses from Clinical Stool Samples in Aichi, Japan
... isolate with 100% bootstrap support. As 5 ⬘ UTR sequences tend to be highly conserved and therefore not entirely suitable for serotyping (31, 38), development of a more sensitive RT- PCR protocol for the VP1 region is ...
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Pentaplex PCR Assay for Detection of Hemorrhagic Bacteria from Stool Samples
... This study has some limitations. First, our assay detects only a limited number of clinically significant pathogens and does not detect protozoa or viral targets. Second, the assay did not detect enterotoxigenic ...
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Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples
... interkingdom RNA regulatory network by modulating microbial and human gene expression levels (17, ...microbial RNA sequences can be detected in transcriptomic experiments performed on human sam- ples (19, ...
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Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples
... human RNA biomarkers to better distinguish subjects with colonic adenoma or carcinoma from healthy ...of RNA from stool samples can provide combinatorial information on the small ...
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Metagenomic Analyses of Viruses in Stool Samples from Children with Acute Flaccid Paralysis
... analyzed viral nucleic acids in stool samples collected from 35 South Asian children with nonpolio acute flaccid paralysis ...nuclease-resistant viral nucleic acids were followed by DNA ...
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Broadly Reactive Nested Reverse Transcription PCR Using an Internal RNA Standard Control for Detection of Noroviruses in Stool Samples
... degree of variability between genotypes, ranging from 2.1 ⫻ 10 6 (Leeds strain) to 39 (Bristol strain) virus particles/ml by nRT-PCR versus 2.1 ⫻ 10 6 virus particles/ml (corresponding to 3.4 ⫻ 10 4 virus ...
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Diversity of Human Parechoviruses Isolated from Stool Samples Collected from Thai Children with Acute Gastroenteritis
... screening stool samples collected from children with acute gastroenteritis in Chiang Mai, Thailand, for infection with HPeV, one of the less explored viral pathogens which has recently been ...
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Multicenter Comparison of Nucleic Acid Extraction Methods for Detection of Severe Acute Respiratory Syndrome Coronavirus RNA in Stool Specimens
... diluted samples; however, the crossing point (the cycle thresh- old number) was shifted later in some cases by as much as three cycles, which represents a 1-log quantity of ...inhibited samples may still ...
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mRNA Extraction from Gill Tissue for RNA-sequencing
... mRNA extraction from fish gills and ...purification from crude cell lysates. This protocol has been adapted from the original protocol for oligo- dT Dynabeads from Invitrogen and was ...
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Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
... arise from the exceptionally robust nature of the oocyst’s walls, which necessitates more stringent treatments for disruption and recovery of DNA for analysis using molecular ...oocysts, from spiked ...
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Nucleocapsid-Independent Specific Viral RNA Packaging via Viral Envelope Protein and Viral RNA Signal
... PS5B190 RNA transcripts were indeed packaged within the VLPs, the samples containing the released VLPs were treated with RNase ...the RNA tran- scripts are present within the VLPs, then the RNAs ...
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Extraction of DNA from Difficult Samples with an Automatable Portable System
... extracted from soil allows conclusions on the composition of the microflora and the purification and analysis of DNA from specific ...for extraction of DNA from difficult samples are ...
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Automated Extraction of Viral Pathogen RNA and DNA for High Throughput Quantitative Real Time PCR
... 24 samples due to the capacity of our ...48 samples, two consecutive batches of 24 samples had to be ...serum samples, a 114-min decrease in total time and 210-min de- crease of hands-on time ...
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Detection of equine infectious anemia viral RNA in plasma samples from recently infected and long term inapparent carrier animals by PCR
... of viral copy number in long-term EIAV- infected horses may be in the same order of magnitude as that found in some HIV-1 patients (43, 44, ...freedom from disease simply by exerting greater control over ...
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Viral RNA / DNA isolation
... cell-free body fluids such as serum or plasma. This kit provides reagents and magnetic beads for isolation of 96 samples from 200 μL serum or plasma. The procedure is based on the reversible adsorption of ...
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