Whole Genome Amplification (WGA)
Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue optimization for genome wide array analyses
... The amplification products after WGA clearly dif- fered in DNA yield and quality (Table 1 and Figure ...BioScore™ WGA kit is developed for formalin- fixed, paraffin-embedded tissue and as a ...
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Efficiency of whole genome amplification of single circulating tumor cells enriched by CellSearch and sorted by FACS
... single-cell whole genome amplification (WGA) is quality ...the amplification reaction that can be followed in real time as shown in Figure ...
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Whole genome amplification and exome sequencing of archived schistosome miracidia
... the genome that are problem- atic to align are removed, (iii) contaminating sequences amplified from FTA cards ...the genome size from 363 to 15 Mb allows sequencing to high read depth, providing robust ...
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A systematic evaluation of whole genome amplification of bisulfite-modified DNA
... Whole genome amplification of bisulfite-modified DNA We performed multiple displacement amplification (MDA)-based WGA by phi29 DNA polymerase using sodium bisulfite-modified DNA as the ...
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Forensic Applications of Whole Genome Amplification
... A review of the literature relating to the forensic applications of whole genome amplification (WGA) has been carried out. DNA profiling techniques are undoubtedly a valuable forensic ...
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Whole genome amplification and its impact on CGH array profiles
... that WGA does not introduce any major distortion of imbalance of gDNA when using CGH ...after amplification (gDNA ...of WGA has to be taken into account when considering this method for array CGH ...
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Impact of Contaminating DNA in Whole Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis
... use WGA as a method to amplify DNA for metagenomic analysis of materials dislodged from the surfaces of explanted prosthetic joint components using sonication ...cases. WGA is one tool available to amplify ...
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Highly efficient PCR assay to discriminate allelic DNA methylation status using whole genome amplification
... Since HM-WGA-PCR is basically identical to HM- PCR except for the WGA process, both methods should provide the same results on the same amplicon. We thus examined whether or not HM-WGA-PCR can dis- ...
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A streamlined workflow for single-cells genome-wide copy-number profiling by low-pass sequencing of LM-PCR whole-genome amplification products.
... of genome representation by Ampli1™ WGA size-selected fragments, genomic DNA from 21 WBCs from 8 individuals was amplified and sequenced on Ion Torrent™ PGM ...Table). Genome was divided into 500kb ...
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Microfluidics-Based Enrichment and Whole-Genome Amplification Enable Strain-Level Resolution for Airway Metagenomics
... Conclusions. The application of our MEEA method leads to gigabases of sequencing reads from airway microbial samples with a limited bias and cost increase. The higher yield and high-quality data ensure more accurate ...
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Selective Whole Genome Amplification for Resequencing Target Microbial Species from Complex Natural Samples
... mitochondrial genome and only motifs with one or more mismatches in the 39 end were selected to reduce mitochondrial sequence ...Selective whole genome ...
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Picodroplet partitioned whole genome amplification of low biomass samples preserves genomic diversity for metagenomic analysis
... We demonstrate that by partitioning the MDA reaction in an emulsion of millions of picoliter-sized droplet reac- tion chambers, we amplify a mixed microbial species sam- ple more uniformly than when the reaction is ...
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Copy number analysis by low coverage whole genome sequencing using ultra low-input DNA from formalin-fixed paraffin embedded tumor tissue
... is whole genome amplification (WGA) to increase the amount of starting template DNA ...the amplification process, potentially causing misinterpretation of the CN profile ...
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N6 methyldeoxyadenosine directs nucleosome positioning in Tetrahymena DNA
... unbiased whole-genome amplification (WGA) with a small amount of native genomic DNA (~ 5 ng) (Additional file 1: Figure S3c), which was followed by nucleosome assembly using the amplified DNA ...
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Genome sequence of Coxiella burnetii strain Namibia
... of whole genome amplification ...single whole genome se- quence and avoids creating and sequencing an additional long distance mate-pair library usually needed to scaffold highly ...
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Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification
... falciparum genome, a published PERL script [8] was used to select up to 100 (8–12 mer) primers with a pre- dicted specified melting temperature (≤30 ...3D7 genome were compared to the contaminating (C) ...
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Selective Whole Genome Amplification Is a Robust Method That Enables Scalable Whole Genome Sequencing of Plasmodium vivax from Unprocessed Clinical Samples
... Eighteen whole-blood samples used for additional testing and further sequencing analysis were derived from whole-blood samples collected from patients with symptomatic ...microscopists. Whole-blood ...
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Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B ( p15 ) gene
... 02/26). Whole genome amplification (WGA) was performed as described previously ...of WGA is to ensure that the DNA is completely unmethylated, as normal, healthy individuals may have ...
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Isothermal Amplification and Molecular Typing of the Obligate Intracellular Pathogen Mycobacterium leprae Isolated from Tissues of Unknown Origins
... multiple-displacement amplification to allow access to a high-quality DNA ...evaluated amplification fidelity, genome coverage, and regional amplification ...global amplification ...
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Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome
... process: whole genome amplification and the Sequenom SNP (single nucleotide polymor- phism) genotyping assay in order to facilitate whole genome mapping of ...
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