[PDF] Top 20 A developmental framework for induced pluripotency

... and drastically decreases the reprogramming efficiency (Ichida et al., 2009; Maherali and Hochedlinger, 2009). Conversely, the inhibition of TGF β signaling by using a TGF β 1 receptor inhibitor or the ectopic expression ... See full document

... plete pluripotency. Although the pluripotency of most iPS cell lines that were tested correlated well with the expression status of these genes, some exceptions existed, in particular Oct4, Sox2 and Klf4 ... See full document

... ‘ Pluripotency: From Basic Science to Therapeutic Applications ’ brought together an inspiring mix of speakers ...controlling pluripotency and the application of this knowledge to study and treat disease ... See full document

... generating induced pluripotent stem cells (iPSCs) (Takahashi and Yamanaka, ...in developmental models ranging from yeast to ...of developmental decisions and is likely to change our current methods ... See full document

... Appropriate downstream applications of iPS cells for developing in vitro disease models or therapeutic purposes are absolutely dependent on the fact that artifi cially derived iPS cells do indeed behave like ESCs under ... See full document

... core pluripotency transcription factors such as OCT3/4 and SOX2 are able to destabilize the existing order in the original cells and re-construct a new ...the pluripotency transcription network, it is ... See full document

... embryonic pluripotency, the consequences of re-expressing Oct4 in somitogenesis-stage embryos were examined using mice carrying an Oct4 transgene (TgOct4) that is ubiquitously expressed in response to doxycycline ... See full document

... of pluripotency-associated markers, including Oct-4, NANOG, and SOX2 and reduction of tumorigenicity in vitro, indicating the suc- cessful reprogramming from malignancy into ...critical pluripotency-associ- ... See full document

... conceptual framework that could be used to derive a flexible and simple collaborative scripting language that could be used in supporting collaboration sessions in a for mal ...conceptual framework is ... See full document

... As cells undergo senescence, oxidative stress and the resultant elevation of reactive oxygen species (ROS) including hydrogen peroxide (H 2 O 2 ) are known to be critical factors involved in the process [10]. In ... See full document

... proposed conceptual framework is composed of four main layers. The first two layers were based on collaboration techniques and the mini-activities within. The third layer has formulated the structure and rules for ... See full document

... So, we’re reaching out to make sure that this tool is available to those who see that there are alternatives, there is better ways doing things, and are needing better ways of function[r] ... See full document

... For example, if the goal is to bring more and more of the world under the control of one particular central planning system the empire of Genghis Khan, the world of Islam, the People’s R[r] ... See full document

... We slowly transitioned our research-grade lines from a xeno-containing substrate and media to xeno-free condi- tions that maintained the pluripotent capability and func- tionality of the hiPSCs. Transitioning of ... See full document

... The objectives of this project were three-fold: the survey would orient the energy manager to the perspective and behaviors of the faculty and students on campus, sociology students wo[r] ... See full document

... To understand the relationships between the different fea- tures of ChIP-seq peaks, we calculated the Spearman rank correlation coefficient values between these features using all human and mouse genes with signal in at ... See full document

... In this paper, we presented a formal healthcare IoT framework. This approach enables us to present an innovative convergence of scalable and interoperable system modules; since standardisation facilitates ... See full document

... The presence of spliced HERV-K(HML-2) type 2 tran- scripts in all pluripotent stem cell lines tested prompted us to investigate whether they encode functional Rec protein and thus enable translation of HERV-K(HML-2) Gag ... See full document

... In addition to estimating the rates of transition between states, it is important to understand the sources that drive and resist transitions. Thus, the mechanisms that control the stability of states and generate ... See full document

... and induced mitochondrial respiratory dysfunction, followed by severely injured mitochondrial ...patient-derived induced pluripotent stem cells (iPSCs) carrying ~100 % mutant ... See full document