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[PDF] Top 20 Alphavirus Nucleocapsid Protein Contains a Putative Coiled Coil α-Helix Important for Core Assembly

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Alphavirus Nucleocapsid Protein Contains a Putative Coiled Coil α-Helix Important for Core Assembly

Alphavirus Nucleocapsid Protein Contains a Putative Coiled Coil α-Helix Important for Core Assembly

... for helix I in alphavirus assembly. The analysis of helix I has suggested that it is important for NC assembly, perhaps functioning to stabilize CP-CP interactions during and ... See full document

10

Alphavirus Capsid Protein Helix I Controls a Checkpoint in Nucleocapsid Core Assembly

Alphavirus Capsid Protein Helix I Controls a Checkpoint in Nucleocapsid Core Assembly

... Domain-swapped helix I mutants of SINV CP can form phenotypically mixed ...in core as- ...the helix I had a prominent role in NC assembly and was proposed to stabilize cores through a ... See full document

8

In Vitro Assembly of Alphavirus Cores by Using Nucleocapsid Protein Expressed in Escherichia coli

In Vitro Assembly of Alphavirus Cores by Using Nucleocapsid Protein Expressed in Escherichia coli

... also contains a series of conserved hydropho- bic and polar residues that are predicted to form an amphi- pathic a helix (residues 38 to 55) based on modeling of the primary sequence into a helical wheel ... See full document

11

The C-Terminal α-Helix Domain of Apolipoprotein E Is Required for Interaction with Nonstructural Protein 5A and Assembly of Hepatitis C Virus

The C-Terminal α-Helix Domain of Apolipoprotein E Is Required for Interaction with Nonstructural Protein 5A and Assembly of Hepatitis C Virus

... same assembly and secretory pathway as VLDL (18, 20, 44, ...the assembly and secretion of ...HCV assembly takes place in the cell in association with lipid droplets (LD) together with HCV C and NS5A ... See full document

10

Alphavirus Entry, Assembly, and Egress.

Alphavirus Entry, Assembly, and Egress.

... Section 3.1-Summary of mutants made: Deletion and substitution mutants were made for each location of interest. The deletion mutants (ΔH230 and ΔG209) should show the effects of the absence of that amino acid residue at ... See full document

81

The Coiled-Coil Domain of the Adenovirus Type 5 Protein IX Is Dispensable for Capsid Incorporation and Thermostability

The Coiled-Coil Domain of the Adenovirus Type 5 Protein IX Is Dispensable for Capsid Incorporation and Thermostability

... employed site-directed mutagenesis (QuikChange site-di- rected mutagenesis kit; Stratagene) of the expression plasmid pAd5pIX (21) to generate the following deletion mutant pro- teins: pIX ⌬ 18-44, which has a deletion ... See full document

5

Head to tail interactions of the coiled coil domains regulate ClpB cooperation with Hsp70 in protein disaggregation

Head to tail interactions of the coiled coil domains regulate ClpB cooperation with Hsp70 in protein disaggregation

... Carroni, M. and Kummer, E. and Oguchi, Y. and Wendler, P. and Clare, D.K. and Sinning, I. and Kopp, J. and Mogk, A. and Bukau, B. and Saibil, Helen R. (2014) Head-to-tail interactions of the coiled-coil ... See full document

37

Cell death and transcriptional signalling mediated by the coiled-coil domain of the barley resistance protein MLA

Cell death and transcriptional signalling mediated by the coiled-coil domain of the barley resistance protein MLA

... a coiled-coil (CC) domain at the N-terminus is a plant-NLR-specific feature and defines two major types of plant NLRs termed the TIR-type NLRs (TNLs) and the CC-type NLRs (CNLs), ...plant protein ... See full document

252

Role of Murine Leukemia Virus Nucleocapsid Protein in Virus Assembly

Role of Murine Leukemia Virus Nucleocapsid Protein in Virus Assembly

... retroviral nucleocapsid protein (NC) originates by cleavage of the Gag ...and contains one or two zinc ...particle assembly and ...Gag protein is still targeted to the plasma ... See full document

8

In Vitro-Assembled Alphavirus Core-Like Particles Maintain a Structure Similar to That of Nucleocapsid Cores in Mature Virus

In Vitro-Assembled Alphavirus Core-Like Particles Maintain a Structure Similar to That of Nucleocapsid Cores in Mature Virus

... cytoplasmic nucleocapsid cores and ...cytoplasmic nucleocapsid cores or CLPs were purified by sedimentation centrifugation ...The nucleocapsid core from the previously published whole-virus ... See full document

5

Amphipathic α helix mimetics through asymmetric self assembly on a metal scaffold

Amphipathic α helix mimetics through asymmetric self assembly on a metal scaffold

... A strategy for the selective formation of anti-parallel triplex metallohelices has been successfully established though both molecular modelling, synthetic and structural studies. Ultimately, while absolute configuration ... See full document

232

HBV RNA pre-genome encodes specific motifs that mediate interactions with the viral core protein that promote nucleocapsid assembly

HBV RNA pre-genome encodes specific motifs that mediate interactions with the viral core protein that promote nucleocapsid assembly

... during assembly, we hypothesize that similar mechanistic constraints may contribute to formation of its ...an assembly competent form of Cp, effectively creating an assembly initiation complex for NC ... See full document

22

The conserved N-terminal region of Sendai virus nucleocapsid protein NP is required for nucleocapsid assembly.

The conserved N-terminal region of Sendai virus nucleocapsid protein NP is required for nucleocapsid assembly.

... To determine the relative amounts of assembled versus unassembled NP protein, extracts from transfected or Sendai virus-infected cells were separated on 4-ml CsCl step gradients.. Gradie[r] ... See full document

10

The Autographa californica Multiple Nucleopolyhedrovirus ac83 Gene Contains a cis-Acting Element That Is Essential for Nucleocapsid Assembly

The Autographa californica Multiple Nucleopolyhedrovirus ac83 Gene Contains a cis-Acting Element That Is Essential for Nucleocapsid Assembly

... NAE contains several conserved AT-rich ...viral protein complexes and are required for viral DNA packaging ...the nucleocapsid assembly mechanisms of linear DNA viruses have been ... See full document

18

Analyses of Coronavirus Assembly Interactions with Interspecies Membrane and Nucleocapsid Protein Chimeras

Analyses of Coronavirus Assembly Interactions with Interspecies Membrane and Nucleocapsid Protein Chimeras

... S protein than did those of ...S protein approaching those of the wild type. Thus, the level of S-protein incorporation into various SARS-CoV M- protein chimeras was consistent with their ... See full document

12

A statistical study of the helix-coil transition of agarose

A statistical study of the helix-coil transition of agarose

... from coil to helix with raising temperature [17] and the structural transition induced in DMSO depends on the concentration of the ...of helix decreases when the temperature variation increases as ... See full document

8

Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe

Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe

... meiotic coiled-coil genes have revealed their pivotal roles in proper meiotic progression, although the significance of the coiled-coil motif for their functions remains elu- ...the ... See full document

8

Catalytic Core of Alphavirus Nonstructural Protein nsP4 Possesses Terminal Adenylyltransferase Activity

Catalytic Core of Alphavirus Nonstructural Protein nsP4 Possesses Terminal Adenylyltransferase Activity

... catalytic core due to sequence similarities with other poly- merases, the presence of conserved polymerase motifs, second- ary structure predictions, and various temperature-sensitive mutations mapping in this ... See full document

8

quick-to-court, a Drosophila Mutant With Elevated Levels of Sexual Behavior, Is Defective in a Predicted Coiled-Coil Protein

quick-to-court, a Drosophila Mutant With Elevated Levels of Sexual Behavior, Is Defective in a Predicted Coiled-Coil Protein

... Identification of a novel gene spanning the site of downstream from an AATAAA hexanucleotide. the P-element insertion: Genomic DNA flanking the The qtc sequence reveals an open reading frame of insertion site of the ... See full document

11

Structural Plasticity of the Coiled-Coil Domain of Rotavirus NSP4

Structural Plasticity of the Coiled-Coil Domain of Rotavirus NSP4

... The CCD likely dictates the structure-function of NSP4. The NSP4 CCD with its unique structural properties, a conserved hep- tad repeat, the unique combination of E120 and Q123, and a pH- sensitive E120, which not only ... See full document

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