Top PDF An Amino Acid in the Stalk Domain of N1 Neuraminidase Is Critical for Enzymatic Activity
An Amino Acid in the Stalk Domain of N1 Neuraminidase Is Critical for Enzymatic Activity
... low enzymatic activity and, in doing so, identify possible molecular markers associated with optimal ...436. Amino acid 19 was located in the TMD, while 232, 248, and 436 were in the globular ...
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Amino Acid 316 of Hemagglutinin and the Neuraminidase Stalk Length Influence Virulence of H9N2 Influenza Virus in Chickens and Mice
... NA activity, but it is compensated by the decreased receptor binding affinity of HA, which is contributed by glyco- sylation at position 170, to restore the functional balance of HA and NA ...short stalk ...
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Role of Transmembrane Domain and Cytoplasmic Tail Amino Acid Sequences of Influenza A Virus Neuraminidase in Raft Association and Virus Budding
... The amino acid sequences of the CT and TMD of NA are highly and moderately conserved, respectively, among the in- fluenza A ...these amino acid sequences in virus biology remain ...specific ...
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Amino Acid Residues Contributing to the Substrate Specificity of the Influenza A Virus Neuraminidase
... sialic acid, and neuraminidase (NA), which removes terminal sialic acid from ...sialic acid and galactose (NeuAc ␣ 2-3Gal and NeuAc ␣ 2-6Gal) and for different molecular species of sialic ...
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A Single Amino Acid Alteration in the Human Parainfluenza Virus Type 3 Hemagglutinin-Neuraminidase Glycoprotein Confers Resistance to the Inhibitory Effects of Zanamivir on Receptor Binding and Neuraminidase Activity
... the neuraminidase and receptor-bind- ing activities, most members of the Paramyxoviridae family possess a dual-function HN ...and neuraminidase func- tions of the paramyxovirus HN molecule has been debated ...
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Identification of Residues That Affect Oligomerization and/or Enzymatic Activity of Influenza Virus H5N1 Neuraminidase Proteins
... tetramerization domain, is an important determinant for maintaining the enzymatic properties within the head ...the enzymatic activity of the N1 protein by affecting oligomerization of ...
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The Fusion Protein Specificity of the Parainfluenza Virus Hemagglutinin-Neuraminidase Protein Is Not Solely Defined by the Primary Structure of Its Stalk Domain
... head domain initially conceals the HN stalk domain but exposes it after the head domain has bound to the receptors, which allows particular amino acids in the stalk domain ...
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Amino Acid Residues in the Cytoplasmic Domain of the Mason-Pfizer Monkey Virus Glycoprotein Critical for Its Incorporation into Virions
... In order to investigate the ability of the mutated glycopro- teins to be incorporated into virus, the mutated env genes were cloned into an infectious molecular clone, pSARM4. The re- sults of these studies showed that ...
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Amino Acid Substitutions in the F-Specific Domain in the Stalk of the Newcastle Disease Virus HN Protein Modulate Fusion and Interfere with Its Interaction with the F Protein
... Amino acid substitutions for P93 modulate fusion and the HN-F interaction but also result in a marked decrease in NA ...HN activity, respectively, when coexpressed with the F ...
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Changes in the Length of the Neuraminidase Stalk Region Impact H7N9 Virulence in Mice
... NA stalk of H7N9 virus did not have a significant impact on NA activity, viral replication in vitro and in vivo, or pathogenesis in mice, even though this deletion reduces the virus release from CRBC and ...
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A carboxy terminal truncation of human alpha galactosidase A in a heterozygous female with Fabry disease and modification of the enzymatic activity by the carboxy terminal domain Increased, reduced, or absent enzyme activity depending on number of amino acid residues deleted
... single amino acid substitutions which are located in the C-terminal region are known to lead to an atypical variant in males, with manifestations limited to the ...C-terminal domain by generating ...
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Identification of a Region in the Stalk Domain of the Nipah Virus Receptor Binding Protein That Is Critical for Fusion Activation
... sialic acid moieties on target cells through two regions of its globular ...its stalk domain triggers F to undergo the conformational changes that render it competent to mediate fusion of the viral ...
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Conserved charged amino acid residues in the extracellular region of sodium/iodide symporter are critical for iodide transport activity
... charged amino acids showed a dramatic reduction in Vmax, while most of the mutants mutated at the negatively charged residues dis- played an increase in ...basic amino acid residues in the ...
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Scanning Mutagenesis Identifies Amino Acid Residues Essential for the in Vivo Activity of the Escherichia coli DnaJ (Hsp40) J-Domain
... contiguous critical residues 33 HPDRN 37 is noteworthy since mutation of resi- dues 38–40 (QGD) following this pentapeptide had no measurable phenotypic effect (Table ...
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Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.
... minimal domain required for the RdRp activity, a series of amino- or carboxy-terminal deletions was con- ...While amino-terminal deletions fused carboxy termi- nally to the His 6 affinity tag ...
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Substrate Binding by the Second Sialic Acid-Binding Site of Influenza A Virus N1 Neuraminidase Contributes to Enzymatic Activity
... (IAV) neuraminidase (NA) protein plays an essen- tial role in the release of virus particles from cells and decoy ...matic activity presumably needs to match the activity of the IAV hemagglutinin ...
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A 27-Amino-Acid Deletion in the Neuraminidase Stalk Supports Replication of an Avian H2N2 Influenza A Virus in the Respiratory Tract of Chickens
... glutamic acid), occurred four amino acids upstream of the original qa-mall/178 adaptive mutation N155D, and in close proximity based on the pre- dicted protein ...
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Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif.
... An antipeptide antibody directed against the wild-type core amino acid segment containing the YGDD region of the poliovirus polymerase reacted with the wild-type recombinant RNA polymera[r] ...
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Pathogenicity and amino acid sequences of hemagglutinin cleavage site and neuraminidase stalk of differently passaged H9N2 avian influenza virus in broilers
... and Neuraminidase Stalk Sequences in H9N2 RT-PCR amplification of a part of HA1 and NA stalk of the extracted RNA of H9N2 recovered from allantoic fluid of eggs that were inoculated with the ...
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Mutagenesis of Paramyxovirus Hemagglutinin-Neuraminidase Membrane-Proximal Stalk Region Influences Stability, Receptor Binding, and Neuraminidase Activity
... (4HB) stalk have not been ...membrane-proximal stalk region (MPSR) (residues 37 to 58) of HN in the context of headless and full- length HN membrane fusion ...the stalk to regulate ...and ...
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