[PDF] Top 20 Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization
... from positive blood culture identification with low ...304 blood cultures demonstrated that cutoffs could be dropped to ⱖ ...566 blood cultures con- sisting of both GN and GP ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... monomicrobial blood cul- ture isolates ...for blood culture analysis which have reported correct identification rates ranging from 67 to 100%, with higher success rates for Gram-negative organisms than for ... See full document
5
Direct Bacterial Identification in Positive Blood Cultures by Use of Two Commercial Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for the identification of bacteria and fungi was recently ... See full document
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Evaluation of a Semiquantitative Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Rapid Antimicrobial Susceptibility Testing of Positive Blood Cultures
... incubation time, concentration of the substance, and the antibiotic-specific time-kill ...test performance from the aerobic ...MALDI-TOF mass spectrometer are the same as those applied for the ... See full document
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Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... critical. Matrix-assisted laser desorption ioniza- tion–time of flight (MALDI-TOF) mass spectrometry is a relatively novel technique used for identification of ... See full document
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Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... Another limitation in our study is that we selected for the MALDI-TOF analysis all the CoNS identified to the species level, meaning those with an identification score of ⱖ 2. However, dur- ing this study period, it ... See full document
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Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... fungal cultures, processing individual isolates “on demand” is cumbersome and batch processing slows turnaround time, abro- gating one of the main advantages of MALDI-TOF ...period, matrix material ... See full document
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Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... host blood in female midges blurred the spectra during the first few days after the blood meal ...for use for use as a high-throughput, accurate, and reproducible identifi- cation of ... See full document
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Lineage and Serotype Identification of Listeria monocytogenes by Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry
... acquisition. Mass spectral analyses were carried out using Autoflex speed instrument (Bruker Daltonics) operated in the linear positive mode using Flex Control ...with laser frequency of 1 kHz, ... See full document
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Rapid Identification of Positive Blood Cultures by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using Prewarmed Agar Plates
... for analysis. The short incubation times did not produce discern- ible colonies but rather a visible film on the plate. The surface of each plate was scraped using a 1-l disposable plastic loop and analyzed by Vitek MS, ... See full document
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Successful Identification of Clinical Dermatophyte and Neoscytalidium Species by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... Using the Voyager DE Pro MS system (Applied Biosystems, Darmstadt, Germany) and the SARAMIS spectral database (Ag- nosTec, Potsdam, Germany), Erhard and coworkers succeeded in identifying five dermatophyte species (T. ... See full document
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Evaluation of the Andromas Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Aerobically Growing Gram Positive Bacilli
... 38). Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a rapid bacterial identification technique that is ... See full document
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Evaluation of the Bruker Biotyper Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Blood Isolates of Acinetobacter Species
... available matrix-as- sisted laser desorption ionization–time of flight mass spectrome- try (MALDI-TOF MS) systems are rapidly emerging novel technology widely used in ... See full document
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Identification of Mycobacteria from Solid and Liquid Media by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in the Clinical Laboratory
... the matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) Biotyper system (Bruker Daltonics) to 16S rRNA/hsp65 ... See full document
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Prospective Evaluation of a Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench by Bench Study for Assessing the Impact on Time to Identification and Cost Effectiveness
... inoculation: blood cultures; sterile body fluid cultures; urine cultures; stool cultures; aerobic wound cultures; an- aerobic wound and tissue cultures; respiratory tract ... See full document
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... MALDI matrix (a saturated solution of ␣ -cyano-4-hydroxy-cinnamic acid in 50% ...temperature. Mass spectra were acquired using the MALDI-TOF spectrometer in a linear positive mode (Mi- croflex; ... See full document
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Utility of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification
... Biotyper, like any identification system, has limitations even for common clinical isolates, and the identifications obtained must be interpreted by experienced laboratory personnel in conjunction with clinical ... See full document
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Performance of Two Resin Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems
... with blood containing therapeutic levels of commonly used antibiotics and antifungal agents ...complex, time-consuming extraction protocols before undergoing direct MALDI-TOF MS assay for species-level iso- ... See full document
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Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
... However, S. pneumoniae was not detected by MALDI- TOF MS using the BD BC system when lysis buffer was added, and we could not detect any bacilli in this BC broth. However, regardless of the extraction method used in ... See full document
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Rapid Identification of Bacteria and Yeasts from Positive Blood Culture Bottles by Using a Lysis Filtration Method and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrum Analysis with the SARAMIS Database
... a positive blood culture would decrease the time to directed antimicrobial therapy and greatly improve patient ...care. Matrix-assisted laser desorption ... See full document
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