[PDF] Top 20 Cell Biological and Functional Characterization of the Vaccinia Virus F10 Kinase: Implications for the Mechanism of Virion Morphogenesis
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Cell Biological and Functional Characterization of the Vaccinia Virus F10 Kinase: Implications for the Mechanism of Virion Morphogenesis
... of F10 expres- sion during v ⌬ iF10 infections at 37°C, we examined serial di- lutions of these extracts by immunoblot ...of virus construction. An inducible copy of the F10 gene, regulated by its ... See full document
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Genetic and Cell Biological Characterization of the Vaccinia Virus A30 and G7 Phosphoproteins
... viral morphogenesis and so performed execution point stud- ies to identify temperature-sensitive stages that were down- stream of the arrest induced by ...infectious virus was produced, and EM analysis ... See full document
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Functional characterization of the vaccinia virus I5 protein
... a functional characterization of the role of the I5 protein in viral replication, we generated the viral recombinant vΔindI5V5 (see Fig ...wild-type vaccinia virus, as we have done before for ... See full document
11
Inhibition of HeLa Cell Protein Synthesis by the Vaccinia Virion
... The very rapid inhibition of HeLa cell protein synthesis after vaccinia infection, the multiplicity effect, the ability to establish the block in the presence of actinomycin D and cycloh[r] ... See full document
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Vaccinia Virus A6 Is Essential for Virion Membrane Biogenesis and Localization of Virion Membrane Proteins to Sites of Virion Assembly
... is vaccinia vi- rus (VACV), which encodes more than 200 proteins in a 190-kb ...VACV virion assembly involves a series of intermediate steps that are discernible by electron microscopy (EM) (reviewed in ... See full document
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Elucidating the Essential Role of the A14 Phosphoprotein in Vaccinia Virus Morphogenesis: Construction and Characterization of a Tetracycline-Inducible Recombinant
... localized in a ring that surrounds the central, lucent core of the virion (21). Taken together, these data suggest that the nucleo- protein complex may associate with proteins located on the inner face the viral ... See full document
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Characterization of Vaccinia Virus Intracellular Cores: Implications for Viral Uncoating and Core Structure
... ferent morphological appearances, can be distinguished in in- fected cells. However, these studies were performed before the VV genome was sequenced, and many of the proteins associ- ated with these uncoating ... See full document
12
Morphological, Chemical, and Biological Characterization of Japanese Encephalitis Virus Virion and Its Hemagglutinin
... The main HA peak at the density region of 1.18 to 1.19 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Three morphologically distinct structures, inner core, envelope, [r] ... See full document
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Characterization of the Determinants of NS2-3-Independent Virion Morphogenesis of Pestiviruses
... to virion morphogenesis in the absence of uncleaved ...molecular characterization of this virus derivative revealed that this chimera still requires NS2 for virion morphogenesis, ... See full document
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Vaccinia Virus A6L Encodes a Virion Core Protein Required for Formation of Mature Virion
... Vaccinia virus A6L is a previously uncharacterized gene that is conserved in all sequenced vertebrate ...recombinant vaccinia virus encoding A6 with an epitope tag and showed that A6 was ... See full document
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Mechanism of Poly (A) Synthesis by Vaccinia Virus
... If vaccinia cores synthesize polyA by transcribing a polydT template, then UV irradiation should inhibit the synthesis of polyA as well as the synthesis of RNA.. To test this, cores were[r] ... See full document
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Identification and Characterization of the Pseudorabies Virus Tegument Proteins UL46 and UL47: Role for UL47 in Virion Morphogenesis in the Cytoplasm
... simplex virus type 1 (HSV-1) constitute major components of the viral ...pseudorabies virus (PrV) were identified in virus-infected cells and in ...nucleus. Virus mutants lacking either the ... See full document
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Vaccinia virus gene D8 encodes a virion transmembrane protein.
... B Amino acid sequence of the D8 protein synthesized in cells infected with a virus possessing a frameshift mutation in gene D8.. The double lines refer to the first 248 amino acids of th[r] ... See full document
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Deletion of the Vaccinia Virus I2 Protein Interrupts Virion Morphogenesis, Leading to Retention of the Scaffold Protein and Mislocalization of Membrane-Associated Entry Proteins
... cells infected with vΔI2 (Fig. 4C and D), demonstrating that the effect on morphogen- esis could be overcome solely by the expression of I2. By manually counting particles in thin sections of 100 RK-13 cells at 18 h ... See full document
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Vaccinia Virus WR Gene A5L Is Required for Morphogenesis of Mature Virions
... recombinant vaccinia virus with an in- ducible A5L ...the vaccinia virus WR A5L open reading frame (corresponding to A4L in vaccinia virus Copenhagen) by the insertion of color ... See full document
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Vaccinia virus gene H5R encodes a protein that is phosphorylated by the multisubstrate vaccinia virus B1R protein kinase.
... S2-Sa kinase purified from HeLa cells infected with vaccinia virus ...of vaccinia virus which they showed to be the product of gene ...protein kinase and to determine whether it ... See full document
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Cell-free translation of purified virion-associated high-molecular-weight RNA synthesized in vitro by vaccinia virus.
... Purified vaccinia virion-associated HMW RNA, synthesized under recently established conditions which permit the preferential synthesis of these large transcripts 29a, was tested for the [r] ... See full document
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Similar Effect of Rifampin and Other Rifamycin Derivatives on Vaccinia Virus Morphogenesis
... Although the mutant was selected from wild-type stock only for its resistance to 100 ,ug of rifampin per ml, no structures resembling MLD formed when the mutantinfected cells were treate[r] ... See full document
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Vaccinia virus B1 kinase: phenotypic analysis of temperature-sensitive mutants and enzymatic characterization of recombinant proteins.
... It synthesi Ls, L cells were infected with either wt virus, ts2, or seemed plausible that the mutant Bi proteins might be more ts25 at ain MOI of 15 PFU per cell and maintained at 32 or [r] ... See full document
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Regulation of Vaccinia Virus Morphogenesis: Phosphorylation of the A14L and A17L Membrane Proteins and C-Terminal Truncation of the A17L Protein Are Dependent on the F10L Kinase
... with vaccinia virus and then probed with an antibody to the peptide corresponding to amino acids 26 to 37, which would be con- served in processed forms of the A17L protein, or with an antibody to the ... See full document
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