[PDF] Top 20 Biophysics and Protein Engineering with Noncanonical Amino Acids
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Biophysics and Protein Engineering with Noncanonical Amino Acids
... the protein to be labeled must be known in advance of the experiment, and the DNA encoding the protein of interest must be genetically modified in order to enable ...of amino acids that can be ... See full document
312
Protein modification through in vivo incorporation of noncanonical amino acids
... cognate amino acid isoleucine (Ile) from the natural amino acid valine (Val), which differs in chemical structure by only one methylene ...misactivated amino acid in an editing site located ~34 Å ... See full document
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Discovery of Aminoacyl-tRNA Synthetase Mutants for the Incorporation of Non Canonical Amino Acids into Proteins
... to noncanonical amino acids through mutations in the binding pocket suggests that changing the binding specificity alone may be sufficient for new AARS ... See full document
205
Revealing, Illuminating, and Modifying Proteins in Human Diseases using Noncanonical Amino Acids
... of amino acids, and they perform many cellular functions, such as replicating genetic materials, catalyzing metabolic reactions, and destroying ...in protein function and regulation are ... See full document
156
Protein engineering via site specific incorporation of nonnatural amino acids
... nonnatural amino acids, the cells containing Mj TyrRS variants recognizing natural amino acids resulted in cell death due to expression of the barnase ...nonnatural amino acids ... See full document
233
Noncanonical Amino Acids in the Interrogation of Cellular Protein Synthesis
... machinery. The selectivity of the method can be enhanced through the use of mutant aminoacyl tRNA synthetases (aaRSs) that permit incorporation of ncAAs not used by the endogenous biomachinery. Through expression of ... See full document
142
Autophagy Related Protein ATG8 Has a Noncanonical Function for Apicoplast Inheritance in Toxoplasma gondii
... To assess the impact of TgATG8 depletion on the T. gondii lytic cycle, a plaque assay was performed. The capacity of parental, mutant, and complemented parasites to produce lysis plaques in a monolayer of host cells was ... See full document
13
Protein Digestion in Human Intestine as Reflected in Luminal, Mucosal, and Plasma Amino Acid Concentrations after Meals
... the protein. No protein was detected in intestinal fluids when the protein-free meal was ...the protein-rich meal, total concentrations of measured free and peptide amino acids ... See full document
10
The Virulence of 1997 H5N1 Influenza Viruses in the Mouse Model Is Increased by Correcting a Defect in Their NS1 Proteins
... NS1 protein, CPSF30 binding by the NS1 protein of H1N1 viruses appears to be less critical for optimal suppression of the host antiviral ...NS1 protein of the 2009 H1N1 virus does not bind CPSF30, ... See full document
11
Noncanonical G Protein Dependent Modulation of Osteoclast Differentiation and Bone Resorption Mediated by Pasteurella multocida Toxin
... mitogen-activated protein (MAP) kinases, Akt ki- nase, or PI3 kinases, leading to stimulation of transcription factors such as AP-1 and NF-B ...G protein- coupled receptors (GPCR) on osteoblasts, which can ... See full document
9
The cloning and characterisation of link1: A LIM-domain containing protein kinase
... and protein kinase C lie within the proline/serine-rich region connecting the LIM and kinase ...and protein kinase C are key regulatory molecules of overlapping ...the protein kinases MAPKAP-2 ... See full document
151
Nontraditional Localization and Retention Signals Localize Human Cytomegalovirus pUL34 to the Nucleus
... containing amino acid sequences that resemble consensus NLSs, UL34 localizes to and is retained in the nucleus by using a nonconventional 204- amino-acid signal comprised of amino acids 40 to ... See full document
6
Short term amino acid infusion improves protein balance in critically ill patients
... parenteral amino acid supply on top of ongoing nutrition in critically ill patients during the first week of intensive care unit (ICU) ...splanchnic protein extraction during ongoing ...parenteral ... See full document
10
Roles of the highly conserved amino acids in the second receptor binding site of the Newcastle disease virus HN protein
... entire phase of membrane fusion is usually divided into three phases: hemi-fusion, pore formation, and pore ex- pansion [25]. The ability of HN mutants to promote cell fusion was evaluated with syncytium formation assay, ... See full document
13
Molecular basis of Fce receptor : IgE interactions
... The FceRI-y subunit is an integral membrane protein with an extracellular region of five amino acids, a transmembrane region of 21 amino acids (as does FceRI-a), and a cytoplasmic regi[r] ... See full document
281
Synthesis of Unnatural Amino Acids for Protein Labeling and Activation.
... 20 amino acids and three translation termination ...the protein synthesis process. RNA-directed synthesis of a protein, called translation, requires transfer RNA (tRNA) to carry amino ... See full document
241
Studies of the Cause and Treatment of Hyperammonemia in Females With Ornithine Transcarbamylase Deficiency
... mented with oral essential amino acids, and a low-protein diet plus oral ketoacids of essential amino acids was.. compared in short-term balance studies; on a separate.[r] ... See full document
10
The N- and C-Terminal Domains of the NS1 Protein of Influenza B Virus Can Independently Inhibit IRF-3 and Beta Interferon Promoter Activation
... 20% amino acid sequence ...of protein kinase R in vitro. A critical function of the A/NS1 protein is the inhibition of synthesis of alpha/beta interferon (IFN- ␣ /  ) during viral ...B/NS1 ... See full document
9
Cloning, expression and location of RNase9 in human epididymis
... J L was responsible for gene cloning, recombinant protein expression, antibody preparation, ribonuclease activity assay and wrote the manuscript. JYL, HYW supervised the work, participated in experimental design, ... See full document
6
Microfluidics for protein biophysics
... Figure 2: A series of scenarios that take advantage of single-phase laminar flow properties at the microscale. a) Plasma proteins are separated from large particles at a branching point of capillaries in the context of ... See full document
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