[PDF] Top 20 Canine mammary tumors contain cancer stem like cells and form spheroids with an embryonic stem cell signature

... single cells in addition to the ...of spheroids and the number of single ...with cells enriched for SP cells, the spheroids became large and after repeated SP analyses the cells ... See full document

... the mammary gland, and when implanted into the fat pads of NOD/SCID mice, induced tumor ...of spheroids varied in subsequent studies [25–33]. Cells isolated from the REM134 canine ... See full document

... five cell lines representative from each primary tumor and compared their expression with normal mammary gland from C57/Bl6 ...all cell lines except for ...B.15 cells, as com- pared with ... See full document

... germ cell that can prolifer- atein vitro and generate oocytes both in vivo and in vitro ...germ cells that express cell-surface RNA heli- case, Vasa, a marker of adult human oogonial stem ... See full document

... the cancer stem cells was the discovery of salinomycin that significantly reduces cell viability of human breast cancer stem-cell- like cells resistant to ... See full document

... OSE cells using non-radioactive Digoxigenin based alkaline phosphatase system by in situ hybridization (Roche Diagnostics, Germany) ...OSE cell smears. Cells were fixed in 2% paraformaldehyde in DPBS ... See full document

... Embryonic stem cells (ESC) have unleashed new avenues in the field of developmental biology and emerged as a potential tool to understand the molecular mechanisms taking place during the process of ... See full document

... the cell spheroids was analyzed qualita- tively by a Live/Dead Kit assay (Molecular Probes, Eugene, USA) on day ...The cell spheroids were washed twice with PBS, followed by suspension in 1 mL ... See full document

... pluripotent stem cells will likely focus more on disease treatment while embryonic stem cells will concentrate on debilitating injuries and degenerative disorders (Hanley, Rastegarlari, ... See full document

... with stem-cell characteristics and plays an important role in tumorigenesis and oogenesis ...thecal cells, and ovarian surface epithelial ...lar cells, GCs show the most common type of ... See full document

... many stem cells migrating as much as ...some cells at the border of the lesion and some migrating cells expressed the chemokine receptor CXCR4, an alpha-chemokine receptor specific for ... See full document

... precancerous stem cells have the fol- lowing ...colon cancer goes through mild, moderate and severe dysplasia, adenoma, carcinoma in situ, to invasive cancer and metastasis ...cerous ... See full document

... as cancer-associated gene expression signatures might reflect the cell of origin of cancer ...Unlike stem cells in the state of CNS neurospheres that are recovered from the CNS, ... See full document

... to form the blastocyst (Fig. 1) comprising of inner cell mass (ICM) and ...of cells form the inner core sandwiched between polar trophblast and hypoblast cells ... See full document

... ASCs secrete different cytokines and growth factors similar to those released by BM-derived MSCs [85]. These properties make ASCs a common source for cell treatment, and currently they are applied in diverse ... See full document

... Stem cell has the ability to divide and self-renew indefinitely as well as to differentiate into one or more cell types ...between embryonic stem cells, which are obtained from ... See full document

... to cell cycle arrest and senescence [82]; the other way is that some dam- age factors like reactive oxygen species are responsi- ble for senescence ...the cells reach ...bladder cancer ... See full document

... Crucially, the identification of oocytes from co-expres- sion of germ-cell markers with EGFP immunofluores- cence in experiments using the TgOG2 mouse [12,13] rests on the exclusivity of expression of Oct4-EGFP in ... See full document

... Mesenchymal stem cells (MSCs) can be recruited to the tumor microenvironment and are known as tumor- associated ...of cancer stem cells ...ovarian cancer stromal progenitor ... See full document

... One study that investigated the use of transplanted mesenchymal SC in cardiac disease therapy showed a reduction in infarct size in the heart and improved cardiac function due to increased left ventricular ejection ... See full document