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[PDF] Top 20 Cell-free expression systems for the construction of artificial cells

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Cell-free expression systems for the construction of artificial cells

Cell-free expression systems for the construction of artificial cells

... and expression of an enzyme to be tested in an assay would provide significant information on how different GC content in the mRNA sequence could affect the protein output ...transcription systems are ...to ... See full document

100

Stögbauer, Tobias Roland
  

(2012):


	Experiment and quantitative modeling of cell-free gene expression dynamics.


Dissertation, LMU München: Fakultät für Physik

Stögbauer, Tobias Roland (2012): Experiment and quantitative modeling of cell-free gene expression dynamics. Dissertation, LMU München: Fakultät für Physik

... the expression in the presence of an artificial lipid membrane system which is advantageous for certain appli- ...a cell-free system can integrate into lipid membranes in situ and fold ... See full document

126

Computational Programming for Product Designing in Synthetic Biology

Computational Programming for Product Designing in Synthetic Biology

... living cells, which contain nucleic acids (DNA and RNA), proteins, carbohydrates, lipids, vitamins and ...the cell is chemicals and all these components have a specific degree of freedom, conformation and ... See full document

9

Cell-free soluble expression of the membrane protein PsbS

Cell-free soluble expression of the membrane protein PsbS

... recombinant expression systems using host cells, but which could be further explored in CF synthesis, where selective lipids can be added to the synthesis reaction or during the subsequent refolding ... See full document

12

Epigallocathechin gallate, polyphenol present in green tea, inhibits stem like characteristics and epithelial mesenchymal transition in nasopharyngeal cancer cell lines

Epigallocathechin gallate, polyphenol present in green tea, inhibits stem like characteristics and epithelial mesenchymal transition in nasopharyngeal cancer cell lines

... of cells called cancer stem-like cells (CSC) or tumor-initiating cells (TIC) ...These cells are generally thought of as self-renewing cells that are able to reinitiate a tumor for ... See full document

12

Cell Free DNA and CD38 Expression on T helper Cells as Biomarkers of Disease Activity in Rheumatoid Arthritis

Cell Free DNA and CD38 Expression on T helper Cells as Biomarkers of Disease Activity in Rheumatoid Arthritis

... Comparison of the 3 groups of the study showed significant difference in levels of plasma cfDNA, being highest in active RA patients (p<0.001) (Table, 3) (Figure, 3A). This finding was in agreement with Bartoloni et ... See full document

9

Efficient Transduction by an Amphotropic Retrovirus Vector Is Dependent on High-Level Expression of the Cell Surface Virus Receptor

Efficient Transduction by an Amphotropic Retrovirus Vector Is Dependent on High-Level Expression of the Cell Surface Virus Receptor

... 208F cells promotes efficient transduction. We transduced 208F cells with the retrovirus vector LPit2SN, which contains the rat Pit2 cDNA under transcriptional control of an MoMLV long terminal repeat ... See full document

6

Studies on rabbit reticulocyte ribosomes and polyribosomes and their relation to hemoglobin synthesis

Studies on rabbit reticulocyte ribosomes and polyribosomes and their relation to hemoglobin synthesis

... For this reason the dissertation is divided into three sections: Part I on ribosomes in cell-free synthesis systems derived from reticulocytes, Part II on hemoglobin synthesis in cells i[r] ... See full document

139

Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury

Significance of downregulation of renal organic cation transporter (SLC47A1) in cisplatin-induced proximal tubular injury

... tubular cells were pretreated with a-HSA or AGE-a-HSA for 24 hours and incubated in vehicle or a cisplatin containing medium for 24 ...The cell viability was measured from absorbance, and the viability ... See full document

6

Antioxidative potential of folate producing and cholesterol removing probiotic Lactobacillus species

Antioxidative potential of folate producing and cholesterol removing probiotic Lactobacillus species

... prepare cell-free extract (CFE) [11]. For preparation of heat killed cells (HKCs), the cell pellets were washed twice, re-suspended in 20 ml of phosphate-buffered saline (PBS, pH ... See full document

7

The influence of the penetrating cryoprotectors on protein synthesis in the cell-free system of the rat liver

The influence of the penetrating cryoprotectors on protein synthesis in the cell-free system of the rat liver

... Необходимо отметить, что после удаления ДМСО уровень биосинтеза протеинов не только возвращается к прежним значениям, но и превышает их на 33%, что, возможно, связано с влиянием дан­[r] ... See full document

8

Protein Synthesis in Cell-Free Systems: an Effect of Interferon

Protein Synthesis in Cell-Free Systems: an Effect of Interferon

... Stimulation of amino acid incorporation by EMC RNA with untreated "74S" ribosomes from control chick embryo fibroblasts CEF, U and CEF which had been exposed to 35 0 and 140 A units of i[r] ... See full document

12

Hypoxic regulation of the expression of cell proliferation related genes in U87 glioma cells upon inhibition of IRE1 signaling enzyme

Hypoxic regulation of the expression of cell proliferation related genes in U87 glioma cells upon inhibition of IRE1 signaling enzyme

... the expression of studied proliferation related genes by hypoxia, we investi- gated the effect of hypoxic condition on gene expres- sions in glioma cells without both enzymatic activi- ties of this ... See full document

11

Construction of an Optimized Helper Plasmid Containing Vpx for Lentiviral-Mediated Transduction of Dendritic Cells

Construction of an Optimized Helper Plasmid Containing Vpx for Lentiviral-Mediated Transduction of Dendritic Cells

... to expression of a sequence Rev and Vpx protein in the form a bicistronic ...Besides, expression of Vpx and Rev on the same plasmid allows preserving the number of helper plasmids that is characteristic of ... See full document

6

In vitro resolution of adeno-associated virus DNA hairpin termini by wild-type Rep protein is inhibited by a dominant-negative mutant of rep.

In vitro resolution of adeno-associated virus DNA hairpin termini by wild-type Rep protein is inhibited by a dominant-negative mutant of rep.

... We expressed both wild-type Rep78 and mutant Rep78NTP proteins in two helper-free expression systems consisting of either recombinant baculoviruses in insect cells or the human immunodef[r] ... See full document

5

Entry of Human T-Cell Leukemia Virus Type 1 Is Augmented by Heparin Sulfate Proteoglycans Bearing Short Heparin-Like Structures

Entry of Human T-Cell Leukemia Virus Type 1 Is Augmented by Heparin Sulfate Proteoglycans Bearing Short Heparin-Like Structures

... HTLV-1 cell tropism has yet to be ...human cell lines to HTLV-1 by using vesicular stomatitis virus pseudotypes bearing HTLV-1 envelope ...the expression of GLUT1, HS, or NRP-1 ...1-transduced ... See full document

11

Autoreproductive cells and plant meristem construction: the case of the tomato cap meristem

Autoreproductive cells and plant meristem construction: the case of the tomato cap meristem

... Then, according to both anatomical observations and theoretical simulations obtained by the application of graph-L-systems, sequen- tial production of descendents from each a-c[r] ... See full document

15

An in vitro Biomolecular Breadboard for Prototyping Synthetic Biological Circuits

An in vitro Biomolecular Breadboard for Prototyping Synthetic Biological Circuits

... the cell-free TX-TL expression system was done according to previously described protocols, resulting in extract with conditions: ...of expression at 29 ° ... See full document

213

Translation of murine leukemia virus RNA in cell-free systems from animal cells.

Translation of murine leukemia virus RNA in cell-free systems from animal cells.

... Fingerprint analysis of tryptic digests of polypeptide products formed in the cell-free system in B Polypeptides 60 and 70K synthesized in the L-cell-free system to 140,000-molecular-wei[r] ... See full document

9

The glycolytic oscillator

The glycolytic oscillator

... The demonstration of oscillations in glycolysis of yeast cells and in cell-free extracts of yeast was the first indication that periodic activities of biological systems might be caused [r] ... See full document

8

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