[PDF] Top 20 Characterization of Essential Domains and Plasticity of the Classical Swine Fever Virus Core Protein
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Characterization of Essential Domains and Plasticity of the Classical Swine Fever Virus Core Protein
... were essential for particle formation, indicating a necessity of positively charged amino acids at this ...of Core protein is its highly positive charge, its function is not solely dependent on basic ... See full document
9
African Swine Fever Virus Protein p17 Is Essential for the Progression of Viral Membrane Precursors toward Icosahedral Intermediates
... African swine fever virus (ASFV) assembly is the appearance of precursor viral membranes, thought to derive from the endoplasmic reticulum, within the assembly ...that protein p54, a viral ... See full document
16
Plasticity of the HEK-293 cells, related to the culture media, as platform to produce a subunit vaccine against classical swine fever virus
... CD154 protein (Toledo et al. 2007; Pujol et al. 2015). This last protein has been used as a molecular adjuvant since several studies have claimed that it potentiates the immune system response (Xiang et ... See full document
10
Classical Swine Fever Virus p7 Protein Is a Viroporin Involved in Virulence in Swine
... forming protein due to its similarities to hepatitis C virus (HCV) p7 ...influenza virus M2 proteins, may function as a viroporin involved in pore formation and transport of ions across cell ... See full document
14
African Swine Fever Virus Proteinase Is Essential for Core Maturation and Infectivity
... African swine fever virus (ASFV) encodes two polyprotein precursors named pp220 and pp62 that are sequentially processed during viral infection, giving rise to six major structural ...the core ... See full document
7
Characterization and molecular basis of heterogeneity of the African swine fever virus envelope protein p54.
... RODRIGUEZ,3 Centro de Investigacion en Sanidad Animal, Instituto Nacional de Investigaci6n y Tecnologia Agraria y Alimentaria, Valdeolmos 28130,1 and Centro de Biologia Molecular "Severo[r] ... See full document
9
Proteomic analysis of swine serum following highly virulent classical swine fever virus infection
... correction. Protein iden- tification was performed by searching the NCBInr database ...search. Protein iden- tification results were filtered with Xcorr(1 + ≥ ... See full document
7
Generation and Efficacy Evaluation of a Recombinant Pseudorabies Virus Variant Expressing the E2 Protein of Classical Swine Fever Virus in Pigs
... glyco- protein E2 of CSFV into the genome of rPRVTJ-delgE under the transcriptional control of the human cytomegalovirus (hCMV) FIG 5 Detection of CSFV-specific antibodies in immunized/challenged pigs using ... See full document
9
In vitroinfection with classical swine fever virus inhibits the transcription of immune response genes
... Mini Protein Tetra system (Bio- Rad). Protein samples were separated by SDS-PAGE and then electroblotted onto an Immobilon-P R Transfer (MILLIPORE) membrane using a Semi-Dry Transfer Sys- tem (Bio-Rad) for ... See full document
11
Poly(C)-Binding Protein 1, a Novel Npro-Interacting Protein Involved in Classical Swine Fever Virus Growth
... of protein expression ...structural protein expression, we examined CSFV C protein expression when PCBP1 was knocked down in PK-15 ...C protein com- pared to that in the cells treated with ... See full document
9
Cytopathogenicity of classical swine fever virus caused by defective interfering particles.
... the virus was characterized extensively both at the genome and protein ...cp virus was generated, but subsequent control experi- ments with virus from older passages did not result in ...cp ... See full document
7
A Recombinant Classical Swine Fever Virus Stably Expresses a Marker Gene
... E2 protein of CSFV Alfort/187 (20, ...E2 protein was detected with monoclonal antibody HC/TC 26 (3) and antimouse horseradish peroxidase conju- gate (DAKO) and quantified by optical density measurement ... See full document
5
Clustering of classical swine fever virus isolates by codon pair bias
... the virus [59]. Thus, a moderately or low virulent virus has a greater chance of circulating in a pig or wild boar population without being detected ... See full document
11
Inactivation of the RNase Activity of Glycoprotein Erns of Classical Swine Fever Virus Results in a Cytopathogenic Virus
... altered protein processing of the nonstructural NS2-3 protein, resulting in the expression or enhanced expression of NS3, generally accepted as the cause for the induction of cy- topathogenicity in vitro ... See full document
7
Experimental infection of Bama miniature pigs with a highly virulent classical swine fever virus
... the virus stock were prepared in Dulbecco ’ s modification of Eagle ’ s medium (DMEM) and used to infect PK-15 cells (seeded in 96-well plates on the previous day at a density of 10 6 ...to virus) were set ... See full document
7
Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
... mRNA expression within tissues. Our study is one of the first to use this approach. This concept was recently reported for infectious bursal disease virus [24]. To achieve this goal, an internal control gene ... See full document
10
African Swine Fever Virus Polyproteins pp220 and pp62 Assemble into the Core Shell
... developing core shell of normal ASFV ...fide core shell is an asymmetrical domain lim- ited by a lipid envelope and an inner DNA-containing nucle- ...the virus assembly where core maturation ... See full document
10
Alteration of a Second Putative Fusion Peptide of Structural Glycoprotein E2 of Classical Swine Fever Virus Alters Virus Replication and Virulence in Swine
... Prediction of internal fusion peptide II of CSFV E2 and design of mutations. Viral IFPs of class II/III glycoproteins form connect- ing loops in  -domains (25). These loops are enriched in Gly residues and often ... See full document
10
Pathogenesis of classical swine fever: B-lymphocyte deficiency caused by hog cholera virus.
... Already at the onset of disease, viral replication in lymphoid tissues demarcates the germinal centers, and the viral genome remains localized to that site as the disease progresses even[r] ... See full document
5
Characterization of the antigenic domains of the major core protein (p26) of equine infectious anemia virus.
... 1007 sera tested also reacted with the recombinant Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A panel of recombinant trpLE-gag fusion proteins and synthetic peptid[r] ... See full document
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