[PDF] Top 20 Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells

... the cells. Human MSCs were seeded under the same experimental conditions and transfected with BLOCK-iT Oligo at varying concentra- ...of cells transfected was determined using a Cytomics FC500 MPL ... See full document

... Taken together, our results suggest that commercially available pure titanium discs which have been irradiated with a laser beam (Yb-YAG) at 235 J/cm 2 fluence modu- late cellular behavior in a manner that is dependent ... See full document

... of human MSC when compared with PLGA alone, but this was not statistically ...of human MSC when hydroxyapatite-Ps was dispersed in the PLGA or when it was used ...of human MSC was very similar on the ... See full document

... activity, cells were cul- tured in differentiation medium for about 21 ...differentiated cells were detached with lysis buffer (50 mM Tris–HCl [pH ... See full document

... and osteogenic differentiation of human mesenchymal stem cells (MSCs) according to the component ratio of the ...and osteogenic differentiation of ... See full document

... tissue, Mesenchymal Stem Cells (MSCs) are deemed to be the most potential adult stem cells for bone regeneration ...the human body in which MSCs can be obtained, such as bone ... See full document

... promote osteogenic differentiation of mesenchymal stem cells and osteoblasts, but little is known about their effects on human periodontal ligament progenitor cells ...the ... See full document

... osteoblastic differentiation such as OCN synthesis and extracellular calcium de- posits became detectable after 7 days of ...Osteoblastic differentiation of HGMSCs was strictly dependent on BECLIN-1 ... See full document

... and osteogenic potency of three different tissue-derived human MSCs on this synthetic ...that human-derived MSCs obtained from umbilical cord, bone marrow, and adipose tissue all could form colonies, ... See full document

... adipocyte differentiation through repression of adipogenic regulators PPARγ and C/EBPα (Li et ...report osteogenic toxicity following AhR ligand exposure; however, the precise mechanisms are not fully ... See full document

... from stem cells and these exosomes can be used to combat the disease progres- sion [64, ...that human BM-MSCs release exosomes containing miRNAs that upregulate the Wnt pathway, leading to ... See full document

... surrounding cells, thereby re- sulting in Wnt loss-of-function phenotypes ...cing cells (Figure 6B) and was unable to move into cul- ture medium (Figure 6C) during Wls (GPR177) siRNA ...upon ... See full document

... miRNAs play important roles in the regulation of hBMSCs differentiation. miR-141 and miR-200a, for ex- ample, have been reported to regulate osteoblast differen- tiation by targeting the BMP-2 signaling pathway ... See full document

... designing stem cell culture environments in micro-bioreactor formats) ...cultured cells and tissues, allowing high-throughput studies with increased numbers of ex- perimental conditions and replicates while ... See full document

... Remodeling of the skeleton is a continuous pro- cess during a human’s life. In order to replace damaged bone and meet the metabolic needs of the body, the remodeling process requires the coordination of bone resorption ... See full document

... Bone marrow MSCs are maintained in a relative state of quiescence until they are activated by a variety of physi- ological and pathological stimuli. Bone marrow MSCs are capable of proliferating and then differentiating ... See full document

... in human MSCs (hMSCs) during osteogenic differentia- tion in response to chemical stimulation [18, ...the differentiation process of SMSCs or the correlation between ... See full document

... cultures. Stem cells reside in almost all tissues within the human body, where they exhibit various ...These cells are of great importance because they control homeostasis, regeneration, and ... See full document

... by human umbilical cord blood-derived MSCs were able to induce β-catenin nuclear translocation of skin cells, enhancing their proliferation and migration ...by human BMSCs could activate several ... See full document

... immune cells as well as cells of macrophage-osteoclast lineage help the removal of bone debris, antisepsis and preparation of MSCs for next repair ...immune cells and mediators play an important role ... See full document