[PDF] Top 20 Cloning animals by somatic cell nuclear transfer – biological factors
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Cloning animals by somatic cell nuclear transfer – biological factors
... various cell types with the same genetic ...With nuclear transplantation, the somatic donor nucleus carries the specific epigenetic modifications of its tissue type, which must be erased during ... See full document
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Somatic cell nuclear transfer: failures, successes and the challenges ahead
... farm animals, and minimal advancements have been achieved only in the ...impairing cloning could be removed by a single ...wide nuclear remodelling remains a priority in our ...perfect nuclear ... See full document
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Generation of bovine transgenics using somatic cell nuclear transfer
... inherent biological variability within the NT system (oocyte maturation, cell culture and donor cell lines) can add another level of variability ...donor cell type and cell cycle stage ... See full document
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Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors
... donor cell and the recipient ...donor cell and recipient oocyte mtDNA populations result in different amino acids being generated within one ...hand-made cloning, where the somatic donor ... See full document
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Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear transferred embryo development in the intraooplasmic karyoplast injection technique: a review
... donor cell instead of whole somatic cell or whole cell nucleus (with intact integrity of nuclear envelope), whose cytosolic bearer is karyoplast, microsurgically released from disrupted ... See full document
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Specific genetic modifications of domestic animals by gene targeting and animal cloning
... major factors contributing to this immunogenicity are thought to be the sequences of the murine C- and V-regions of an antibody molecule ...B cell development in these mutant mice could be restored by the ... See full document
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The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer
... of nuclear reprogramming following somatic cell nuclear transfer (SCNT) is thought to depend on factors present in the ...the somatic cell type on the outcome of ... See full document
6
Application of new biotechnologies for improvements in swine nutrition and pork production
... embryos, cloning is known as embryonic cell nuclear transfer ...first cloning of pigs through the use of embryonic cells as a ...mature animals, cloning is called ... See full document
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Bähr, Andrea (2011): (Re)producing transgenic pigs for xenotransplantation – selection of founder animals and establishment of breeding herds. Dissertation, LMU München: Tierärztliche Fakultät
... of animals constitute factors that contribute to heterogeneity of experimental results (Sachs in Swindle ...these animals to homozygosity for this gene and establishing a GalKO herd proved difficult ... See full document
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Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)
... Fam60al is negatively regulated by fam60al-AS Pearson correlation analysis revealed a moderate negative correlation between fam60al and fam60al-AS (correlation coefficient: -0.604, P < 0.05), suggesting that ... See full document
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SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo
... immunoassay (Cobas e411, Roche Diagnostics, Mann- heim, Germany). Oocytes were retrieved surgically under general anesthesia after ovulation as reported previously [18]. After retrieval, metaphase II (MII) oocytes were ... See full document
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Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
... Activin A, a member of the TGF-β superfamily, is a functional cytokine of maternal-foetal interac- tion, mainly produced in uterine epithelial cells and endometrial cells (Manova et al., 1992; Orimo et al., 1996; Park et ... See full document
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Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
... for cloning ferrets, we have begun to exam- ine parameters for ferret cloning by nuclear transfer using G0/G1-stage donor fetal fibroblasts ...by nuclear transfer is the complete ... See full document
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Generation of Cashmere Goats Carrying an EDAR Gene Mutant Using CRISPR-Cas9-Mediated Genome Editing
... 89 cell lines obtained through the cultivation of clonal cell lines, 62 were positive for EDAR gene ...these cell lines, EDAR gene-targeted Cashmere goat embryos were prepared by somatic ... See full document
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Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer
... Somatic cell nuclear transfer (SCNT) was performed as previously described ...before nuclear transfer and then injected into the perivi- telline space of an enucleated ...donor ... See full document
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Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis
... crucial factors affecting embryo development ...the nuclear reprogramming of sperm and somatic cell undergoes two distinct ...the nuclear morphology of donor cell, to reverse the ... See full document
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Generation of GHR modified pigs as Laron syndrome models via a dual sgRNAs/Cas9 system and somatic cell nuclear transfer
... PIEC cell were lysed in 10 μl of NP-40 solution for 15 min at 65 °C and 10 min at 95 ...°C. Cell lysates were used as templates for PCR amplification of the targeted region with specific prim- ers ... See full document
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Nuclear Transmutation and Cancer in the Biological Cell
... to nuclear reaction , which can be detected easily at very low levels and with no chance for mistaken identity using the Mössbauer ...in cell cycle arrest, stimulate the NT in the biological ... See full document
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Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer
... GTKO cell colonies’ selection, we successfully obtained three GGTA1 biallelic knockout colonies and produced the cloned piglets with the expected genotype from one GTKO cell ...GGTA1-mutant somatic ... See full document
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Serum starvation-induced cell cycle synchronization stimulated mouse rDNA transcription reactivation during somatic cell reprogramming into iPSCs
... synthesis. Somatic cell reprogramming can be accomplished by a variety of ways, such as nuclear transplantation (nuclear trans- fer) [42, 43], cell fusion [44], and direct reprogram- ... See full document
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