[PDF] Top 20 Coding Sequences Enhance Internal Initiation of Translation by Hepatitis A Virus RNA In Vitro
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Coding Sequences Enhance Internal Initiation of Translation by Hepatitis A Virus RNA In Vitro
... A virus (HAV) belongs to the Picornaviridae fam- ily and is the sole member of the Hepatovirus ...positive-strand RNA of about ...initiate translation. The 59NCR contains an internal ribosome ... See full document
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The 5' nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro.
... Nucleotide substitutions in specific virus strains from the wild-type HM175 sequence are shown in boldface type; base pairs identified by covariant substitutions among all nine strains a[r] ... See full document
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The sequence element of the internal ribosome entry site and a 25-kilodalton cellular protein contribute to efficient internal initiation of translation of hepatitis C virus RNA.
... of hepatitis C virus (HCV) RNA is initiated by internal entry of ribosomes into the 5 * noncoding region ...the internal ribosome entry site (IRES) and may involve host ...for ... See full document
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The 3′-Untranslated Region of Hepatitis C Virus RNA Enhances Translation from an Internal Ribosomal Entry Site
... HCV RNA enhances its translation in monocistronic ...HCV RNA, we compared the translational efficiency of several synthetic RNAs which contain the authentic 59-end sequence of HCV RNA, but ... See full document
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The Hepatitis C Virus RNA 3′-Untranslated Region Strongly Enhances Translation Directed by the Internal Ribosome Entry Site
... For RNA transfections, approximately 0.8 g of each in vitro transcribed RNA was used for transfection of the cells using a TransMessenger transfection kit ...in vitro transcript were mixed ... See full document
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Structural Determinant of Human La Protein Critical for Internal Initiation of Translation of Hepatitis C Virus RNA
... immunodeficiency virus (T-20), in general the potential of drug development increases sharply as the molecular weight of the peptide ...IRES-mediated translation in cell culture at a significantly lower ... See full document
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Interaction of polypyrimidine tract-binding protein with the 5' noncoding region of the hepatitis C virus RNA genome and its functional requirement in internal initiation of translation.
... in translation ini- tiation (47). This mutant RNA did compete for PTB binding to the wild-type 5 9 NCR (data not ...these sequences, recovered translational efficiency of the 5 9 ...this RNA ... See full document
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Translation Initiation at the CUU Codon Is Mediated by the Internal Ribosome Entry Site of an Insect Picorna-Like Virus In Vitro
... by internal ribosome ...PSIV RNA sequence containing the IRES was aligned with the sequences upstream of the capsid-coding regions of DCV and RhPV by using CLUSTAL W (34) ...PSIV RNA of ... See full document
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De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... the virus (6, 50, ...in vitro studies using various recombinant systems (6, ...the initiation of translation of the viral polyprotein (6, 12, 13, ...viral RNA genome (33, 57, ...the ... See full document
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Sequences within a small yeast RNA required for inhibition of internal initiation of translation: interaction with La and other cellular proteins influences its inhibitory activity.
... positive-sense RNA genome of polio- virus (PV), a member of the Picornaviridae family, translates in a unique cap-independent manner in the cytoplasm of infected ...The translation of the viral ... See full document
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A Peptide Derived from RNA Recognition Motif 2 of Human La Protein Binds to Hepatitis C Virus Internal Ribosome Entry Site, Prevents Ribosomal Assembly, and Inhibits Internal Initiation of Translation
... La120-208 coding sequences were PCR amplified using the primers 5⬘-GGCCGGATCCGAAGTGACTTGGGA-3⬘, 5⬘-GGCTGAATT CCAGGTCTGTTTCTTT-3 ⬘ , 5 ⬘ -GCATGGATCCACTGATGCAACTCTT-3 ⬘ , and 5⬘-CGTAGAATTCCTACTGGTCCCAG-3⬘ and ... See full document
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Sequences in the 5′ Nontranslated Region of Hepatitis C Virus Required for RNA Replication
... polyprotein coding region resides in the loop of stem-loop 4 and a pseudoknot structure is formed by sequences at the 3⬘ end of stem-loop ...minus-strand RNA acts as the promoter for ... See full document
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The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codon
... diarrhea virus (BVDV), classical swine fever virus (CSFV) and border disease virus (BDV) are the causative agents of economically important diseases of cattle, pigs and ...to hepatitis C ... See full document
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Hepatitis delta antigens enhance the ribozyme activities of hepatitis delta virus RNA in vivo.
... in vitro and in vivo (16, 33, ...HDV RNA, which has been demonstrated to form an unbranched, rod-like structure ...the RNA exists in alternative RNA conformations ...different RNA ... See full document
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Mechanisms Governing the Selection of Translation Initiation Sites on Foot-and-Mouth Disease Virus RNA
... 48S initiation complex formation in a fractionated system dependent on purified initiation factors are also more consistent with ribosome scanning to the Lb site than any other mechanism, such as direct ... See full document
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Internal initiation of translation on the vesicular stomatitis virus phosphoprotein mRNA yields a second protein.
... VSV mRNA was synthesized in vitro in a reaction mixture containing final concentration Tris hydrochloride pH 8.0 50 mM, NaCI 0.1 M, dithiothreitol 4 mM, MgCl2 5 mM, ATP, CTP, and GTP 1 m[r] ... See full document
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Internal ribosome entry site within hepatitis C virus RNA.
... No RNA lane 1 and capped unmethylated GpppG-linked HCV RNAs that carried the 5' UTRs of group II HCV lane 2 and group I HCV lane 3 were used as mRNAs in a cell-free protein synthesis sys[r] ... See full document
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Effects of alterations in the leader sequence of Rous sarcoma virus RNA on initiation of translation.
... We found that small perturbations of the leader length do not affect the binding and scanning of ribosomal subunits by more than about 10%, that the length and/or structure of the RSV RN[r] ... See full document
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Characterization of replicative intermediate RNA of mouse hepatitis virus: presence of leader RNA sequences on nascent chains.
... These data indicate that the 5' leader sequences are present at the 5' ends of the nascent mRNA strands during transcription, rather than joined to the body sequences posttranscriptional[r] ... See full document
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Two initiation sites for translation of poliovirus RNA in vitro: comparison of LSc and Mahoney strains.
... A comparison of the proteins synthesized in LSc virus-infected cells in the presence or absence of excess salt with those synthesized by Mahoney virus-infected cells showed differences i[r] ... See full document
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