[PDF] Top 20 Comparison of the Mycobacteria Growth Indicator Tube (MGIT) with radiometric and solid culture for recovery of acid fast bacilli

... Specimen processing. In all three centers, respiratory, urine, and gastric fluid specimens were liquefied and decontaminated with NALC-NaOH, as recom- mended by the manufacturer. Lymph nodes and tissue specimens were ... See full document

... the recovery rate for AFB as determined by this study and assuming, in parallel, the same ratio of smear-positive to smear-negative specimens, as was the case in the study of van Griethuysen et ...affects ... See full document

... 5), culture still represents the corner- stone on which a definitive diagnosis of tuberculosis and other mycobacterioses ...for culture detection of acid-fast bacilli has been regarded ... See full document

... for acid-fast bacilli ...showed growth on routinely inoculated mycobacteria growth indicator tube (MGIT) 960 tubes, and multiplex polymerase chain reaction ... See full document

... as culture methods, should accurately confirm the identity of acid-fast bacilli (AFB) present in positive specimens and cultures, and should provide the information in a timely ...in ... See full document

... imens are needed. Some of the noncommercially available direct tests include the nitrate reductase assay (NRA) and microscopic observation drug susceptibility (MODS) assay. These tests have been developed as “in house” ... See full document

... in Peru because these conditions were found in pilot experiments to provide optimal neutralisation and con- centration (data not shown). The pellet from centrifuga- tion was re-suspended in 34 ml PBS and then split into ... See full document

... This study had two strength points. Firstly, it had a larger sample size than other studies. Thus, the results are more reliable. Secondly, it compared pulmonary tuberculosis in both healthy individuals and those with ... See full document

... positive growth on LJ were characterized with Genotype CM/AS assay (Hain Lifescience, Nehren, Germany) the details of the mycobacteria isolation and characterization for this study are described elsewhere ... See full document

... Radiometric antimicrobial susceptibility tests, if performed by using pure culture recovered from a conventional solid medium, required approximately 5 days with 98.6% agreement with the[r] ... See full document

... the MGIT BACTEC 960 for six ...(PNB) acid [20]. All isolates identified as MTB underwent MGIT AST for INH, RIF, streptomycin, and ethambutol, using previ- ously described methods ...positive ... See full document

... of mycobacteria growth indicator tube for direct and indirect drug susceptibility testing of Mycobacterium tuberculosis from respiratory specimens in a siberian prison ... See full document

... Despite recent advances, the smear microscopy remains the cornerstone for the diagnosis of PTB (Pulmonary Tuberculosis) in high burden countries like India (Tiwari, WHO also recommends sputum microscopy WHO, 2009). One ... See full document

... but culture-positive patients however would benefit from DST and it is possible that their exclusion could lead to some ...of culture (as liquid culture may be more sensitive in smear-negative ... See full document

... mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical ...with ... See full document

... the MGIT 960 system versus B460 (by pooling odds ratios for each of the tested drugs and overall) and summarized as the odds ratio plus 95% ...with MGIT 960 (6, 38), only low-concentration-resistant strains ... See full document

... nucleic acid hybridization probe assays for ...traditional culture (2, 4). Among myco- bacterial culture detection systems, the BACTEC 460 TB system has long been the standard against which others ... See full document

... each MGIT containing ...the MGIT 960 instru- ment were incubated at 37°C and automatically monitored for the increase rate of ...each tube location was taken daily at the same ...drug-containing ... See full document

... The purpose of this multicenter study was to evaluate the reliability of the newly introduced BACTEC MGIT 960 system for testing the susceptibility of M. tuberculosis to the three front-line drugs (INH, RIF, and ... See full document

... for culture, stool for acid fast bacilli smear, stool for Mycobacteria tuberculosis culture, stool for Mycobacteria tuberculosis polymerase chain reaction, early morning ... See full document