[PDF] Top 20 Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein
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Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein
... of Rep (Rep1–491 and Rep1–484) fused with PR891-HBD proved hormone dependent, while fusions with full-length Rep78 or Rep68 did ...active site of the heterologous moiety is not unprecedented (35, 40, ... See full document
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Substitution of adeno associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences
... in integration efficiency as indicated by the intensity of bands on our gels of PCR products ...in integration site specificity, which would have been indicated by a reduced size range for the PCR ... See full document
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Asymmetric replication in vitro from a human sequence element is dependent on adeno-associated virus Rep protein.
... of human parvovirus adeno-associated virus type 2 (AAV) integrates preferentially into a defined region of human chromosome ...that integration occurred by a complex, ... See full document
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Packaging of Human Chromosome 19-Specific Adeno-Associated Virus (AAV) Integration Sites in AAV Virions during AAV Wild-Type and Recombinant AAV Vector Production
... of Rep with the AAV-ITR and AAVS1 (31) but also demon- strated Rep-dependent initiation of DNA synthesis starting off a DNA sequence within AAVS1 that serves as the origin se- quence ...of Rep ... See full document
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Development of Animal Models for Adeno-Associated Virus Site-Specific Integration
... The adeno-associated virus (AAV) is unique in its ability to target viral DNA integration to a defined region of human chromosome 19 ...AAV-mediated ... See full document
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Adeno-Associated Virus Site-Specific Integration Is Mediated by Proteins of the Nonhomologous End-Joining Pathway
... Adeno-associated virus (AAV) is a ubiquitous human virus ( ⬃ 80% of adults are seropositive [4]); like other nuclear DNA viruses, it causes persistent ...helper virus ... See full document
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Kinetics and Frequency of Adeno-Associated Virus Site-Specific Integration into Human Chromosome 19 Monitored by Quantitative Real-Time PCR
... Adeno-associated virus (AAV) has evolved a biphasic life cycle to ensure persistence in its primate ...helper virus, adenovirus (Ad) or herpesvirus, for productive infection ...preferential ... See full document
6
Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... (33), human 293 cells, and the Detroit 6 cell line 7374 (4), which is latently infected with wild-type AAV2, were maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine ...fluorescent ... See full document
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A Helper-Dependent Capsid-Modified Adenovirus Vector Expressing Adeno-Associated Virus Rep78 Mediates Site-Specific Integration of a 27-Kilobase Transgene Cassette
... Random integration of viral gene therapy vectors and subsequent activation or disruption of cellular genes poses safety ...vector integration to specific sites in the host genome. The ... See full document
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Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination
... Adeno-associated virus (AAV) type 2 is a nonpathogenic, replication-defective parvovirus that is dependent on superin- fection with helper adenovirus for efficient replication ...chromosomal ... See full document
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Adeno-Associated Virus Type 2 p5 Promoter: a Rep-Regulated DNA Switch Element Functioning in Transcription, Replication, and Site-Specific Integration
... helper virus, AAV2 infection can result in a unique form of latency featuring preferential integration into the human genome at a site termed AAVS1 at human chromosome ...of ... See full document
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Roles of Adeno-Associated Virus Rep Protein and Human Chromosome 19 in Site-Specific Recombination
... Though site-specific integration is dependent upon either of the two large Rep proteins, the AAV ITRs are the only cis elements required for integration (34, 44, ...of Rep ... See full document
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A Chimeric Protein Containing the N Terminus of the Adeno-Associated Virus Rep Protein Recognizes Its Target Site in an In Vivo Assay
... of adeno-associated virus (AAV) type 2 are involved in DNA replication, regulation of gene expression, and targeting site-specific ...a specific Rep recognition sequence ... See full document
11
Site-Specific Integration in Mammalian Cells Mediated by a New Hybrid Baculovirus–Adeno-Associated Virus Vector
... The adeno-associated virus (AAV) is also used for gene delivery ...eukaryotic virus that preferentially integrates its DNA into a defined region of the host cell genome located on chro- mosome ... See full document
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Mutational analysis of the adeno-associated virus rep gene.
... Each of the mutant genes has been expressed in cell culture and assayed for the following functions: i nuclear localization, ii AAV DNA replication, iii trans activation of the AAV capsi[r] ... See full document
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Binding sites for adeno-associated virus Rep proteins within the human genome.
... The Rep proteins of adeno-associated virus type 2 (AAV) are known to bind to Rep recognition sequences (RRSs) in the AAV inverted terminal repeats (ITRs), the AAV p5 promoter, and the ... See full document
7
Identification of linear DNA sequences that specifically bind the adeno-associated virus Rep protein.
... We find that Rep68 is capable of binding to a linear DNA sequence that is contained within a 25-bp sequence of the A stem of the adeno-associated virus AAV terminal repeat proximal to th[r] ... See full document
10
Studies of the Mechanism of Transactivation of the Adeno-Associated Virus p19 Promoter by Rep Protein
... a Rep-expressing plasmid re- pressed p5 transcription both in the absence (20-fold decrease) and in the presence (12-fold decrease) of Ad ...of Rep to the p5 ...by Rep expression both in the presence ... See full document
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Adeno-Associated Virus Rep Represses the Human Integration Site Promoter by Two Pathways That Are Similar to Those Required for the Regulation of the Viral p5 Promoter
... and protein levels from 3 independent experiments were quantitated ...of Rep with the RBS is not sufficient to direct Rep-mediated repression of the PPP1R12C promoter, suggesting the existence of at ... See full document
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Features of the adeno-associated virus origin involved in substrate recognition by the viral Rep protein.
... In addition, the enzyme may recognize the polarity of the two strands at the cut site, or it may recognize another sequence element within the stem of the hairpin that is required for co[r] ... See full document
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