[PDF] Top 20 Consequences of Dimerization of the Voltage-Gated Proton Channel

... The apparent discrepancy between the two suggested dimer interfaces could be explained in at least two ways. In one, the TM domains of the two protomers, although tethered by coiled-coil interactions within the ... See full document

... the consequences of allowing ions to cross membranes differ drastically for each species of ion, we must delve a bit deeper for a more informative ...þ channel; voltage-gated Na þ ... See full document

... cient proton pathways, 23,120−125 although they are not intrinsically ...if proton-selective permeation requires a salt ...the proton that might be expected Figure ... See full document

... produces proton selectivity, Asp frequently engages in salt linkage with one or more Arg residues from ...in proton-selective, anion- permeable, and nonconducting ...produce proton-selective ... See full document

... In most experiments, cells expressing green fluorescent protein (GFP)-tagged proton channels were identified using inverted mi- croscopes (Nikon) with fluorescence capability. For constructs that lacked the GFP ... See full document

... the proton channel gene now enables several new approaches to this ...the proton channel that alter function in intact cells would be very useful, but to my knowledge do not yet ...functional ... See full document

... and proton fluxes are carried through separate molecules, in contrast to a number of molecules important in bioener- getics, in which electron and proton movement is strictly coupled (51, 130, 194, 394, ... See full document

... polarization-activated proton channel (R1 → His), a pro- ton carrier (R2 → His or R3 → His), or a depolarization activation proton channel (R4 → ... See full document

... the channel closes and therefore the current ...the channel after the voltage pulse. The proton chan- nel does not close during sustained depolarizing pulses! Many voltage-gated ... See full document

... Inhibiting proton channels with Zn 2ⴙ resulted in profound acidification to levels that inhibit NADPH ...by proton channels is essential to the production of reactive oxygen species during ... See full document

... Finally, voltage-gated proton channels comprise a major con- ductance in rat alveolar epithelial type II cells (10), are demon- strably present in the apical membranes of cultured cells (12), and ... See full document

... is voltage dependent, in the sense that the ion flux is different at different ...this voltage dependence is distinct from voltage-dependent ...the channel continuously until the ... See full document

... There is growing evidence that H + channels are not identical in all preparations, but differ in several respects. These differences are summarized in table 1. The proposed nomenclature is likely to be ephemeral and is ... See full document

... distinctly to PMA and GFX, whereas the double mutant T9A/S77a also fails to respond. These results implicate Thr 9 as essential to the enhanced gating response. Families of currents in the same cell during identical ... See full document

... and proton chan- nels are evident when one compares the responses of neu- trophils from normal human subjects and from CGD ...between proton channels and CGD was a study showing that stimulation of CGD ... See full document

... endogenous proton currents in non-transfected HEK-293 cells have voltage dependence like that of other native proton currents ...transfected channel protein is unlikely given that H V 1 ... See full document

... hypothetical proton channels (10). Proton entry into the scintillon triggers the flash by two concerted mechanisms: low pH activates luciferase itself (11, 12) and also causes release of the substrate ... See full document

... closed conformation. This structure shows that the SF Asp is located in a hydrophobic layer compris- ing two conserved Phe residues, which might prevent water penetration. Presumably, this hydropho- bic region prevents ... See full document

... mutant channel lost proton spe- cificity and became anion-selective or did not ...remained proton-specific. Mutation of the nearby Asp 185 did not impair proton selectivity, indicating that ... See full document

... examples, proton selectivity is likely achieved by a titratable residue, but such conclusions are not set in ...D channel of cytochrome c oxidase, is almost certainly protonated during normal enzyme opera- ... See full document