[PDF] Top 20 Construction and Immunogenicity of Recombinant Adenovirus Vaccines Expressing the HMW1, HMW2, or Hia Adhesion Protein of Nontypeable Haemophilus influenzae
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Construction and Immunogenicity of Recombinant Adenovirus Vaccines Expressing the HMW1, HMW2, or Hia Adhesion Protein of Nontypeable Haemophilus influenzae
... (26). Recombinant adenoviral vectors are particularly attractive as vaccine candi- dates for immunization of the upper respiratory tract ...(48). Recombinant adenoviral vec- tors have been shown to be ... See full document
9
Antibodies Specific for the Hia Adhesion Proteins of Nontypeable Haemophilus influenzae Mediate Opsonophagocytic Activity
... The Hia autotransporter proteins are highly immunogenic surface adhesins expressed by nontypeable Haemophilus influenzae ...the hia gene that encodes a surface-exposed portion of the H. ... See full document
7
Immunogenicity of Nontypeable Haemophilus influenzae Outer Membrane Vesicles and Protective Ability in the Chinchilla Model of Otitis Media
... of nontypeable Haemophilus influenzae (NTHi) OMV-specific antisera and the pro- tective ability of NTHi OMVs as vaccine antigens in the chinchilla otitis media ...three ... See full document
12
Turnover of nonencapsulated Haemophilus influenzae in the nasopharynges of otitis prone children
... of nontypeable Haemophilus influenzae (NTHI) isolated from the nasopharynges of children with recurrent acute otitis media ...H. influenzae isolates collected prospectively during a 2-year ... See full document
5
Vaccine Candidates against Nontypeable Haemophilus influenzae: a Review
... H. influenzae strains, including encapsulated serotypes a–f and NTHi, have this antigenically conserved 42-kDa protein [57] , making it an attractive vaccine ...H. influenzae [59] , one of which is ... See full document
8
Efficiency of Recombinant Bacille Calmette Guérin in Inducing Humoral and Cell Mediated Immunities against Human Immunodeficiency Virus Type 1 Third Variable Domain in Immunized Mice
... from the immunized mice every 2 weeks were assessed by ELISA as described in the Materials and Methods. Anti-serum was collected from the rBCG- mV3-immunized mice 6 weeks after primary inoculation. (B) Cell lysates or ... See full document
8
Acquired macrolide resistance genes in nontypeable Haemophilus influenzae
... H. influenzae remains to be ...H. influenzae Rd KW20 and examine the phenotypic effect of expression of these ...H. influenzae Rd ...H. influenzae Rd KW20 was transformed with these ... See full document
302
Binding of Complement Regulators to Invasive Nontypeable Haemophilus influenzae Isolates Is Not Increased Compared to Nasopharyngeal Isolates, but Serum Resistance Is Linked to Disease Severity
... To analyze whether NTHi bound vitronectin from NHS, bacteria were grown overnight and incubated with hiNHS and PBS. To remove unbound proteins, NTHi bacteria were washed 5 times with the same buffer. Thereafter, the bac- ... See full document
7
Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice
... circumsporozoite protein (CSP) is the most studied and commonly used antigen for the purpose of developing a vaccine against malaria ...kD protein composed of a C-terminus containing the thrombospondin-like ... See full document
14
An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable <em>Haemophilus influenzae</em> and <em>Streptococcus pneumoniae</em> to cultured human bronchial epithelial cells exposed to cigarette smoke
... Notes: (A) Beas-2B cells exposed to Cse (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. (B) reduced attachment of S. pneumoniae bacterial cells to Cse-treated Beas-2B cells due to ... See full document
9
Dectin 1 Is Expressed in Human Lung and Mediates the Proinflammatory Immune Response to Nontypeable Haemophilus influenzae
... Interestingly, our results also suggest that Dectin-1 directly in- teracts with NTHI and is involved in the NTHI-induced proin- flammatory immune response of bronchial epithelial cells, al- though we did not identify the ... See full document
9
Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia Genes in Haemophilus influenzae Isolates
... of Haemophilus influenzae to respiratory epithelial cells is the first step in the pathogenesis of ...H. influenzae infection and is facilitated by the action of several adhesins located on the ... See full document
8
Prevalence of the sodC Gene in Nontypeable Haemophilus influenzae and Haemophilus haemolyticus by Microarray Based Hybridization
... H. influenzae strains in this study revealed an internal 536- to 548-bp frag- ment, depending on the ...H. influenzae strains were, on average, ...H. influenzae strains and two (C09-23 and P23-21) ... See full document
6
In Silico Modeling of Biofilm Formation by Nontypeable Haemophilus influenzae In Vivo
... ABSTRACT Biofilms formed by nontypeable Haemophilus influenzae (NTHI) bacteria play an important role in multiple respiratory tract diseases. Visual inspection of the morphology of biofilms formed during ... See full document
13
Comparative Immunogenicity in Rhesus Monkeys of DNA Plasmid, Recombinant Vaccinia Virus, and Replication-Defective Adenovirus Vectors Expressing a Human Immunodeficiency Virus Type 1 gag Gene
... or adenovirus type 5 vectors to inhibit viral replication and dis- ease progression in rhesus macaques following challenge with ...the adenovirus type 5 vector either by itself or in a prime-boost ... See full document
9
Immunogenicity of Haemophilus influenzae Type b Capsular Polysaccharide Vaccines in 18-Month-Old Infants
... The Finnish investigators who conducted the efficacy trial con- cluded recently that a concentration of 0.15 sg/ mL of anti-PRP antibody protein as measured by radioimmunoassay was prote[r] ... See full document
6
Relationships of Nontypeable Haemophilus influenzae Strains to Hemolytic and Nonhemolytic Haemophilus haemolyticus Strains
... H. influenzae type strains expressed the opposite phenotypes (with the exception of strain 86-028NP, which lacked ODC ...H. influenzae strains (Table 3 and ...H. influenzae (the prevalence ratios are ... See full document
11
Shielding of a Lipooligosaccharide IgM Epitope Allows Evasion of Neutrophil Mediated Killing of an Invasive Strain of Nontypeable Haemophilus influenzae
... ABSTRACT Nontypeable Haemophilus influenzae is a frequent cause of noninvasive mucosal inflammatory diseases but may also cause invasive diseases, such as sepsis and meningitis, especially in ... See full document
10
Nontypeable Haemophilus influenzae Lipooligosaccharide Expresses a Terminal Ketodeoxyoctanoate In Vivo, Which Can Be Used as a Target for Bactericidal Antibody
... RNA isolation and quantitative real-time PCR. Cell samples were homogenized by pipetting with 1 ml of TRIZOL total RNA isolation reagent (Life Technologies, Grand Island, NY). Lysates were transferred to Lysing Matrix B ... See full document
11
Dual RNA seq of Nontypeable Haemophilus influenzae and Host Cell Transcriptomes Reveals Novel Insights into Host Pathogen Cross Talk
... skeletal protein ␥ -adducin is localized at spectrin-actin junctions; it binds calmodulin and is an in vivo substrate for pro- tein kinase C (PKC) and Rho-associated ...this protein in the loss of ... See full document
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