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[PDF] Top 20 Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish

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Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish

Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish

... significant developmental period of zebrafish SCNT embryos remains to be ...five stages to be chosen, which is too broad to provide this ...of nuclear transferred embryos [12, 22] and leaves ... See full document

11

The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer

The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer

... of nuclear reprogramming following somatic cell nuclear transfer (SCNT) is thought to depend on factors present in the ...the somatic cell type on the outcome of the ... See full document

6

Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)

Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)

... in somatic cell reprogramming of human pluripotent stem cells [25, ...in somatic cell reprogramming, which improved somatic cell induction efficiency by blocking the ... See full document

9

SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo

SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo

... the developmental stages and localization of transferred embryos were analyzed histologi- cally in the surrogate uterus and oviduct 8 days after em- bryo ...SV40LT-K9 cell group were in the oviducts ... See full document

11

Somatic cell nuclear transfer: failures, successes and the challenges ahead

Somatic cell nuclear transfer: failures, successes and the challenges ahead

... spermatid nuclear remodeling in a somatic ...the somatic histones, including H3K9me3, a critical epigenetic barrier of SCNT reprogramming (Iuso et ...traditional nuclear transfer ... See full document

9

Development of genome engineering technologies in cattle: from random to specific

Development of genome engineering technologies in cattle: from random to specific

... initial stages of genome engineering, DNA micro-injection into one-cell stage embryos (zygotes) followed by embryo transfer into a recipient was performed because of the ease of the ...low ... See full document

9

Ras Related Nuclear Protein is required for late developmental stages of retinal cells in zebrafish eyes

Ras Related Nuclear Protein is required for late developmental stages of retinal cells in zebrafish eyes

... of cell cycle exit to undergo differentiation is very ...in cell cycle exit will result in increased numbers of neurons or glial cells which form at the later ...premature cell cycle exist will ... See full document

9

Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer

Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer

... Somatic cell nuclear transfer (SCNT) was performed as previously described ...before nuclear transfer and then injected into the perivi- telline space of an enucleated ...donor ... See full document

11

Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors

Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors

... Interspecies somatic cell nuclear transfer (iSCNT) involves the transfer of a nucleus or cell from one species into the cytoplasm of an enucleated oocyte from ...early ... See full document

13

Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear transferred embryo development in the intraooplasmic karyoplast injection technique: a review

Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear transferred embryo development in the intraooplasmic karyoplast injection technique: a review

... the somatic cloning technique. The alternative strategy of nuclear-cytoplasmic hybrid creation, which has been proposed by Lee et ...whole somatic cell bypassing both the do- nor ... See full document

11

Generation of Cashmere Goats Carrying an EDAR Gene Mutant Using CRISPR-Cas9-Mediated Genome Editing

Generation of Cashmere Goats Carrying an EDAR Gene Mutant Using CRISPR-Cas9-Mediated Genome Editing

... as nuclear donor ...the developmental stages of the embryos revealed that the fusion rate of the EDAR gene-targeted cloned embryos was significantly higher than that of the wild-type embryos (Figure ... See full document

10

Transcriptional profiling of spiny lobster metamorphosis reveals three new additions to the nuclear receptor superfamily

Transcriptional profiling of spiny lobster metamorphosis reveals three new additions to the nuclear receptor superfamily

... The MARs show quite some variation in expression between the three species examined, with the adult tis- sue distribution in S. verreauxi suggesting more consti- tutive expression than in the larvae, where expression ... See full document

14

Bähr, Andrea
  

(2011):


	(Re)producing transgenic pigs for xenotransplantation – selection of founder animals and establishment of breeding herds.


Dissertation, LMU München: Tierärztliche Fakultät

Bähr, Andrea (2011): (Re)producing transgenic pigs for xenotransplantation – selection of founder animals and establishment of breeding herds. Dissertation, LMU München: Tierärztliche Fakultät

... The pig lung has already served as an excellent model for the normal and diseased human lung, or the effect of therapeutics, in many ways (Rogers et al. 2008). Structural and size similarities between porcine and human ... See full document

188

Changes in antioxidant enzyme activities and protein profileduring different stages of somatic embryogenesis in sugarcane (Saccharum officinarum L )var 93v297

Changes in antioxidant enzyme activities and protein profileduring different stages of somatic embryogenesis in sugarcane (Saccharum officinarum L )var 93v297

... during somatic embryogenesis have been reported recently in many species such as peanut (Roja Rani et ...different stages of somatic embryogenesis ...germination stages (55 ...different ... See full document

5

COMPARATIVE GENE EXPRESSION PROFILING DURING IN VITRO REGENERATION IN TWO COCONUT CULTIVARS

COMPARATIVE GENE EXPRESSION PROFILING DURING IN VITRO REGENERATION IN TWO COCONUT CULTIVARS

... different stages of in vitro regeneration in two coconut cultivars ...development stages in the two cultivars were analyzed using ...normal somatic embryos compared to abnormal somatic ... See full document

9

Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis

Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis

... Differentiated cell nuclei can be reprogrammed to a pluripotent state in several ways, including incubation with oocyte extracts, transfer into enucleated oocytes, and induced pluripotent stem cell ... See full document

10

Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research

Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research

... block cell division, and interfere with tissue replenishment, especially in dis- eases of human aging and in some aging-related pro- cesses ...stem cell “ignition” mechanism for tissue regeneration and ... See full document

12

Carbonic anhydrase expression and CO2 excretion during early development in zebrafish Danio rerio

Carbonic anhydrase expression and CO2 excretion during early development in zebrafish Danio rerio

... earliest stages of development ...early stages of development, becoming significantly elevated over the ...mature zebrafish, where zCAb mRNA expression, at ... See full document

9

Generation of GHR modified pigs as Laron syndrome models via a dual sgRNAs/Cas9 system and somatic cell nuclear transfer

Generation of GHR modified pigs as Laron syndrome models via a dual sgRNAs/Cas9 system and somatic cell nuclear transfer

... SCNT: somatic cell nuclear transfer; T7ENI: T7 endonuclease I; q‑PCR: quantitative polymerase chain reaction; WB: western blotting; IHC: immunohistochemical; CRISPR/Cas9: clustered regularly ... See full document

14

Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology

Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology

... PFF cell was transferred into the perivitelline space of the enucleated oocyte, after which the donor cell and recipient cytoplast were fused and acti- vated to form a reconstructed ... See full document

8

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