[PDF] Top 20 Detection and identification of fungal pathogens in blood by using molecular probes
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Detection and identification of fungal pathogens in blood by using molecular probes
... The fungal suspensions with predetermined numbers of CFU per milliliter were centrifuged at 5,000 3 g, and then the pellet was incubated with 5 ml of leukocyte lysis buffer (WCLB) consisting of 10 mM Tris (pH ... See full document
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Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens
... the detection and identification of fungi directly from blood culture specimens that have been flagged as ...the fungal internal transcribed spacer 1 (ITS1) and ITS2 region, elongation factor ... See full document
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Species Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology
... invasive fungal infection, rapid identification of the fungal species is a prerequisite for selection of the most appropriate antifungal ...reliable identification of a wide range of ... See full document
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Prospective Study of the Performance of Vibrational Spectroscopies for Rapid Identification of Bacterial and Fungal Pathogens Recovered from Blood Cultures
... Rapid identification is important because some spe- cies are intrinsically resistant to antifungal agents of the azole group, which are usually the agents of first choice in treating this kind of ...rapid ... See full document
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DNA Microarray Based Detection and Identification of Fungal Pathogens in Clinical Samples from Neutropenic Patients
... invasive fungal infections (IFI) in immunocompromised patients emphasizes the need to improve diagnostic ...14 fungal pathogens (Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, ... See full document
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Development of a DNA Microarray for Detection and Identification of Fungal Pathogens Involved in Invasive Mycoses
... simultaneously. Using two multiplex liquid hybridization mixtures, Hendolin and col- leagues needed 2 days for the detection of eight Candida and Aspergillus species from tissue samples ...simultaneous ... See full document
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Evaluation of the ePlex Blood Culture Identification Panels for Detection of Pathogens in Bloodstream Infections
... of blood culture positivity (6) and has been implemented routinely in our ...rapid detection of resistance mechanisms that may have important consequences for the choices of antimicrobial therapy have been ... See full document
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Simultaneous Detection of Pathogens in Clinical Samples from Patients with Community Acquired Pneumonia by Real Time PCR with Pathogen Specific Molecular Beacon Probes
... sensitivity and high specificity, with the results for six CAP pathogens agreeing well with those of conventional cultural and immunological methods. Furthermore, cases where there were discrepancies between ... See full document
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Clinical relevance of molecular identification of microorganisms and detection of antimicrobial resistance genes in bloodstream infections of paediatric cancer patients
... Methods: Blood samples were processed by the BACTEC system and microbial identification and susceptibility tests were performed by the Phoenix ...evaluated using sex-specific TaqMan probes and ... See full document
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Rapid Identification of Dimorphic and Yeast Like Fungal Pathogens Using Specific DNA Probes
... histologic identification, atypical tissue forms may resemble other fungi (15, ...tissue using molecular probes. First, fungal DNA can be extracted from the tissue and PCR-EIA can be ... See full document
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Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5 8S Ribosomal DNA Typing in Ocular Infections
... with fungal detection in ocular ...a fungal infection should be present in all fungal genera (but should contain enough internal variation in its sequence to define a given species) and should ... See full document
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Molecular probes for diagnosis of fungal infections
... important fungal 28S ...all probes completed our ob- jective of developing an assay with clinical ...universal fungal detection and identification protocol is a sim- ple yet effective ... See full document
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Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens
... the detection of fungi in tissue specimens. The PCR amplified the fungal internal transcribed spacer (ITS) region ...specific probes, eight common fungal pathogens (Aspergillus flavus, ... See full document
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Molecular identification of mango malformation pathogens in Egypt
... 2) using Biometera Uno thermal cycler, as follows: One cycle at 95˚C for 3 min- utes and then 44 cycles at 92˚C for 2 minutes, 37˚C for 1 minute and 72˚C for 2 minutes (for denaturation, an- nealing and extension, ... See full document
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Identification of Parasitic Cysteine Protease Inhibitors using Analog Design, Molecular Docking and Molecular Dynamics Studies
... additional molecular parameters such as values for “conformational search by using the program Frog and maximum number of conformers” was set to 10, number of generations = 25 and population size = 40 ... See full document
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Molecular detection of vector borne pathogens in blood and splenic samples from dogs with splenic disease
... extracted using the Purelink® Genomic DNA Kit following manufacturer instructions (Thermo Fisher Scientific, Waltham, MA, ...whole blood as previously described ...geted using specific PCR assays ... See full document
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Novel chemical method for the preparation of nucleic acids for nonisotopic hybridization
... Compared with the use of radioactive probes, nonisotopic probes using biotin and enzymatic detection techniques are at present less sensitive for the detection of nitrocellulose filter-b[r] ... See full document
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Detection and identification of Treponema hyodysenteriae by using oligodeoxynucleotide probes complementary to 16S rRNA
... No binding to partially purified rRNA or whole-cell rRNA of Treponema innocens, Treponema succinifaciens, Treponema bryantii, or Escherichia coli occurred under stringent conditions.. Th[r] ... See full document
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<p>Identification of dynamic molecular networks in peripheral blood mononuclear cells in type 1 diabetes mellitus</p>
... peripheral blood mononuclear cells ...T1DM, using bioinformatic ...dynamic molecular networks during insulin treatment of T1DM, have not been fully ... See full document
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Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience
... the identification of fungal species is based on the detection of conserved sequences in the rDNA ...detect fungal DNA, but the majority of them (including our group) use as target DNA the ... See full document
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