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[PDF] Top 20 Detection of Mycoplasma hyopneumoniae by using rRNA oligodeoxynucleotide hybridization

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Detection of Mycoplasma hyopneumoniae by using rRNA oligodeoxynucleotide hybridization

Detection of Mycoplasma hyopneumoniae by using rRNA oligodeoxynucleotide hybridization

... hyopneumoniae in clinical samples, such as bronchoalveolar lavage fluid and lung lesions from experimentally infected pigs, was evaluated by this assay.. The evidence obtained from the a[r] ... See full document

6

Detection of Mycoplasma hyopneumoniae in Bronchoalveolar Lavage Fluids of Pigs by PCR

Detection of Mycoplasma hyopneumoniae in Bronchoalveolar Lavage Fluids of Pigs by PCR

... of detection of ...M. hyopneumoniae did not multiply in these cultures and the amplification inhibitors in the medium (for example, the serum content) as well as the fact that the target DNA was not ... See full document

5

Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene

Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene

... 1965 Mycoplasma hyopneumoniae was recognized as the causative agent of enzootic pneumonia in swine (EP) (7, ...M. hyopneumoniae is easily overgrown by other mycoplasmas; Mycoplasma hyorhinis ... See full document

5

First demonstration of the circulation of a pneumovirus in French pigs by detection of anti-swine orthopneumovirus nucleoprotein antibodies

First demonstration of the circulation of a pneumovirus in French pigs by detection of anti-swine orthopneumovirus nucleoprotein antibodies

... and Mycoplasma hyopneumoniae (IDEXX and Oxoid- ThermoFisher Scientific), two major porcine respiratory pathogens [7], had been performed previously to assess the status of the selected pig ... See full document

8

Incidence of Reinfections with Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniaein Pig Farms Located in Respiratory-Disease-Free Regions of Switzerland – Identification and Quantification of Risk Factors

Incidence of Reinfections with Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniaein Pig Farms Located in Respiratory-Disease-Free Regions of Switzerland – Identification and Quantification of Risk Factors

... M. hyopneumoniae and ...environments, detection requires a high level of awareness of disease and report- ing of suspicious, even vague, clinical symp- ... See full document

12

Interaction between Mycoplasma hyopneumoniae and  Swine Influenza Virus

Interaction between Mycoplasma hyopneumoniae and Swine Influenza Virus

... M. hyopneumoniae until 14 DPI (35 days after M. hyopneumoniae challenge), when two of five ...M. hyopneumoniae- infected pigs had seroconverted, while all of the pigs in the DUAL group were ... See full document

6

Serum metabolite markers of early Mycoplasma hyopneumoniae infection in pigs

Serum metabolite markers of early Mycoplasma hyopneumoniae infection in pigs

... M. hyopneumoniae from clinical ...M. hyopneumoniae are most com- monly used to detect exposure ...PCR detection across different sample types varies [13] and many sampling methods are con- sidered ... See full document

10

Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd

Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd

... Serology using ELISA is the preferential approach in order to screen for ...M. hyopneumoniae infections is often low and the positive herd predict- ive value of a serological result decreases progres- ... See full document

6

Detection of Mycoplasma genitalium by PCR Amplification of the 16S rRNA Gene

Detection of Mycoplasma genitalium by PCR Amplification of the 16S rRNA Gene

... MTP-based hybridization assay. Amplicons produced with primers MG16S- 45F–MG16S-447R were labeled with digoxigenin (DIG) during PCR by addition of DIG-11-dUTP to the master mix. The reaction components and cycling ... See full document

6

Current Status of M  hyopneumoniae in Pigs in Grenada, West Indies

Current Status of M hyopneumoniae in Pigs in Grenada, West Indies

... Mycoplasma hyopneumoniae , cause of enzootic pneumonia is known for se- rious economic losses in ...on Mycoplasma hyopneumoniae incidence in Grenadian ...for Mycoplasma ... See full document

9

Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

... M. hyopneumoniae strain 232 to ...M. hyopneumoniae strain 232 with 10 μg of pMHO-1, cells were incubated for 3 h at 37 °C and then plated onto Friis agar containing 2 μg/mL ...M. hyopneumoniae and/or ... See full document

10

Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which mycoplasma hyopneumoniae regulates surface topography

Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which mycoplasma hyopneumoniae regulates surface topography

... mutagenesis using mutagenic primers (see Table S1 in the supplemental ...M. hyopneumoniae strain 232 chromosomal DNA using Pfu polymerase (Aglient Technologies, CA) and cloned into the ... See full document

12

MLVA typing of Mycoplasma hyopneumoniae bacterins and field strains

MLVA typing of Mycoplasma hyopneumoniae bacterins and field strains

... M hyopneumoniae bacterins with M hyopneumoniae fi eld strains, DNA from 28 bronchoalveolar lavages (BAL) positive for the pathogen were included in the ...extracted using a commercial kit (DNAzol, ... See full document

6

Survey on Mycoplasma hyopneumoniae gilt acclimation practices in Europe

Survey on Mycoplasma hyopneumoniae gilt acclimation practices in Europe

... occur and that the maternally derived antibodies are not fully protective against infection, newborn piglets are sus- ceptible to M. hyopneumoniae colonization [7]. Thus, contact between infected sows and their ... See full document

8

Influence of pig gut microbiota on Mycoplasma hyopneumoniae susceptibility

Influence of pig gut microbiota on Mycoplasma hyopneumoniae susceptibility

... calculated using the entire amplicon sequence variant ...performed using one-way ANOVA and Tukey’s test was used to perform post hoc ...M. hyopneumoniae experimentally inoculated ... See full document

13

A Novel SimpleProbe PCR Assay for Detection of Mutations in the 23S rRNA Gene Associated with Macrolide Resistance in Mycoplasma genitalium in Clinical Samples

A Novel SimpleProbe PCR Assay for Detection of Mutations in the 23S rRNA Gene Associated with Macrolide Resistance in Mycoplasma genitalium in Clinical Samples

... Screening for M. genitalium in asymptomatic patients is con- troversial. The high sensitivity of nucleic acid amplification tests may result in the detection of low-grade infections that have the potential to ... See full document

5

Frey, Tanja
  

(2014):


	Wirksamkeit einer two-shot Vakzine nach experimenteller Infektion von Endmastschweinen mit Mycoplasma hyopneumoniae.


Dissertation, LMU München: Tierärztliche Fakultät

Frey, Tanja (2014): Wirksamkeit einer two-shot Vakzine nach experimenteller Infektion von Endmastschweinen mit Mycoplasma hyopneumoniae. Dissertation, LMU München: Tierärztliche Fakultät

... Erreger Mycoplasma hyopneumoniae ist der primäre Verursacher der weltweit verbreiteten Enzootischen Pneumonie (THACKER, ...Gattung Mycoplasma, der Familie Mycoplasmataceae und der Ordnung der ... See full document

90

Low temperature and dust favour in vitro survival of Mycoplasma hyopneumoniae : time to revisit indirect transmission in pig housing

Low temperature and dust favour in vitro survival of Mycoplasma hyopneumoniae : time to revisit indirect transmission in pig housing

... encountered in pig units, and exposed to temperatures of 4°C, 25°C and 37°C for up to 12 days. Survival of the organisms was determined by recovering the organism from the surface material and culturing in Friis broth. ... See full document

15

Detection of Gallibacterium spp  in Chickens by Fluorescent 16S rRNA In Situ Hybridization

Detection of Gallibacterium spp in Chickens by Fluorescent 16S rRNA In Situ Hybridization

... Fixation of bacterial cells. One hundred microliters of cultured bacterial cells in logarithmic growth phase was fixed in 800 ␮l of 1⫻ phosphate-buffered saline (PBS; Life Technologies, Taastrup, Denmark) with 4% ... See full document

6

PCR based assay for Mycoplasma hyopneumoniae

PCR based assay for Mycoplasma hyopneumoniae

... The kit of claim 2 further comprising a probe compris ing the nucleotide sequence 5‘-GGTAGCCCITCCI'I'I‘GAGGT-3' SEQ ID NO: 3 that hybridizes to an ampli?ed target nucleic acid sequence r[r] ... See full document

14

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