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[PDF] Top 20 Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

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Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

... and cheap Real Time qPCR assays for large-scale epidemiological surveys, based on detection and amplification of gametocyte sex specific transcripts selected from the literature: the ... See full document

11

Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

... and cheap Real Time qPCR assays for large-scale epidemiological surveys, based on detection and amplification of gametocyte sex specific transcripts selected from the literature: the ... See full document

11

Single-Cell Analysis Reveals Distinct Gene Expression and Heterogeneity in Male and Female Plasmodium falciparum Gametocytes

Single-Cell Analysis Reveals Distinct Gene Expression and Heterogeneity in Male and Female Plasmodium falciparum Gametocytes

... the Plasmodium falciparum life cycle, with mature gametocytes being the only form of the parasite capable of human-to-mosquito ...of male and female gametocytes ... See full document

18

A multiplex assay for the sensitive detection and quantification of male and female Plasmodium falciparum gametocytes

A multiplex assay for the sensitive detection and quantification of male and female Plasmodium falciparum gametocytes

... new female gametocyte marker gene, CCp4 was introduced and benchmarked against Pfs25 transcript-based quantifi- ...of male and female game- tocytes and show similar stability under suboptimal stor- ... See full document

11

Biology of Plasmodium falciparum gametocyte sex ratio and implications in malaria parasite transmission

Biology of Plasmodium falciparum gametocyte sex ratio and implications in malaria parasite transmission

... understanding Plasmodium falciparum gametocyte biology and its relationship with malaria parasite transmission, the gametocyte sex ratio contribution to this process still remains a ... See full document

8

Effects of mefloquine and artesunate mefloquine on the emergence, clearance and sex ratio of Plasmodium falciparum gametocytes in malarious children

Effects of mefloquine and artesunate mefloquine on the emergence, clearance and sex ratio of Plasmodium falciparum gametocytes in malarious children

... late gametocytes while being at the same time directly game- tocytocidal to early ...Gametocyte sex ratio may be influenced by a number of factors including anaemia, the number of and the compe- ... See full document

13

Mitosis in the Human Malaria Parasite Plasmodium falciparum

Mitosis in the Human Malaria Parasite Plasmodium falciparum

... the parasite uses one final round of synchronized mitosis at the end of schizogony ...in Plasmodium schizogony, this would indeed be an intriguing ...early Plasmodium mitosis, chromosomes remain ... See full document

9

Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays

Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays

... feeding assays with cultured gametocytes [35] warrants caution when comparing feeders within or between ...of gametocytes as reflected by the PCR ... See full document

9

Immunogenetics and the design of Plasmodium falciparum vaccines for use in malaria endemic populations

Immunogenetics and the design of Plasmodium falciparum vaccines for use in malaria endemic populations

... of populations upon immuniza- tion is also critical to the successful induction of pro- tective ...a human canvas more responsive to vaccines, as negative pathogen immunoregulatory effects are temporarily ... See full document

8

PfMAP-2 is essential for male gametogenesis in the malaria parasite Plasmodium falciparum

PfMAP-2 is essential for male gametogenesis in the malaria parasite Plasmodium falciparum

... for male gametogenesis and further sexual development in the mosquito vector 27 – 29 ...KO gametocytes also show a defect in male gametogenesis by assessing exflagellation rates ...by male ... See full document

16

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex nested RT PCR

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex nested RT PCR

... P. falciparum isolates from patients in Tak Province who had febrile symptom ~3 days (range one to eight days) before blood sample collection (n = 44) revealed that all isolates gave positive results for RT-PCR ... See full document

8

Life span of in vitro differentiated Plasmodium falciparum gametocytes

Life span of in vitro differentiated Plasmodium falciparum gametocytes

... of gametocytes in the circulation is often below the detection limit of commonly used methods and to follow in vivo a single mature gametocyte when released from the site of sequestration to the peripheral ... See full document

10

The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum.

The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum.

... 4). Plasmodium species harbor a relatively high number of candidate mRBPs as compared to other organisms: ...P. falciparum proteome and ...P. falciparum, ... See full document

19

The mRNA bound proteome of the human malaria parasite Plasmodium falciparum

The mRNA bound proteome of the human malaria parasite Plasmodium falciparum

... It is known that UV-crosslinking experiments often yield many false positives as a result of background from proteins that are covalently crosslinked to RNA in a non-specific manner. Recent efforts have been made to ... See full document

18

Targeting NAD + Metabolism in the Human Malaria Parasite Plasmodium falciparum

Targeting NAD + Metabolism in the Human Malaria Parasite Plasmodium falciparum

... the parasite, but did not identify the enzymes involved in de novo synthesis, suggesting that the parasite is a NAD + auxotroph ...hour parasite life cycle as well as showing a steady rate of ... See full document

Multiplication rate variation in the human malaria parasite Plasmodium falciparum.

Multiplication rate variation in the human malaria parasite Plasmodium falciparum.

... . Parasite multiplication rates have been assessed for only a few laboratory adapted clones of ...P. falciparum 13 , and from genetic cross progeny of two clones 14 , but these may not be representative of ... See full document

9

High susceptibility of wild Anopheles funestus to infection with natural Plasmodium falciparum gametocytes using membrane feeding assays

High susceptibility of wild Anopheles funestus to infection with natural Plasmodium falciparum gametocytes using membrane feeding assays

... and robust infections in mosquito ...feeding assays for ethical rea- sons, including avoiding the risk of infection of study participants with cryptic pathogens that may be trans- mitted when using ... See full document

8

High susceptibility of wild Anopheles funestus to infection with natural Plasmodium falciparum gametocytes using membrane feeding assays.

High susceptibility of wild Anopheles funestus to infection with natural Plasmodium falciparum gametocytes using membrane feeding assays.

... and robust infections in mosquito ...feeding assays for ethical rea- sons, including avoiding the risk of infection of study participants with cryptic pathogens that may be trans- mitted when using ... See full document

8

An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes

An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes

... netes produced in media A to D and was much higher for mosquitoes fed with ookinetes produced in medium E (Figure 6). This work sought to reproduce in vitro two essential steps of parasite development. The first ... See full document

7

Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria

Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria

... F. Amado, Sylvie Garcia, et al. To cite this version: Julien Duez, John P. Holleran, Papa Alioune Ndour, Sasdekumar Loganathan, Pascal Amireault, et al.. Splenic retention of Plasmodium falciparum ... See full document

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