[PDF] Top 20 Detection of Treponema pallidum by a sensitive reverse transcriptase PCR
Has 10000 "Detection of Treponema pallidum by a sensitive reverse transcriptase PCR" found on our website. Below are the top 20 most common "Detection of Treponema pallidum by a sensitive reverse transcriptase PCR".
Detection of Treponema pallidum by a sensitive reverse transcriptase PCR
... T. pallidum in CSF. To date, the most sensitive test has been the RIT, in which ...T. pallidum is isolated by inoculation of CSF into the testes of rabbits ...patients. PCR assays present a ... See full document
5
Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment
... Detection by Polymerase Chain Reaction of Treponema pallidum DNA in Cerebrospinal Fluid from Neurosyphilis Patients before and after Antibiotic Treatment GERDA T.. DE JONGE,1 AND JAN D.[r] ... See full document
9
Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers
... multiplex PCR (M-PCR) assay with colorimetric detection was devised for the simultaneous amplification of DNA targets from Haemophilus ducreyi, Treponema pallidum, and herpes simplex ... See full document
6
New Tests for Syphilis: Rational Design of a PCR Method for Detection of Treponema pallidum in Clinical Specimens Using Unique Regions of the DNA Polymerase I Gene
... direct detection of treponemes from clinical specimens has become an important method for the early diagnosis of Treponema pallidum ...direct detection, dark-field microscopy of the ulcers is ... See full document
6
Evaluation of a PCR Test for Detection of Treponema pallidum in Swabs and Blood
... T. pallidum is a highly invasive organism that is able to pene- trate endothelial cell monolayers and to enter the bloodstream in vivo ...T. pallidum genome in whole blood from patients at all stages of ... See full document
7
Performance of the 47 Kilodalton Membrane Protein versus DNA Polymerase I Genes for Detection of Treponema pallidum by PCR in Ulcers
... A.G.-A. designed and coordinated the prospective clinical study, col- lected and managed data, and performed the statistical analysis. P.S., S.L., L.T.-T., B.M.D.T., T.F., and M.C. were responsible for or coordinated ... See full document
5
Sensitive detection of Treponema pallidum by using the polymerase chain reaction
... Positive PCR results were obtained from serum, CSF, and amniotic fluids of untreated syphilis patients but not from control specimens.. Representative results are shown in Fig.[r] ... See full document
8
Clinical Value of Treponema pallidum Real Time PCR for Diagnosis of Syphilis
... a Treponema pallidum real-time TaqMan PCR for the detection of primary and secondary ...T. pallidum real-time PCR for the diagnosis of primary syphilis was evaluated by the use ... See full document
6
Sensitivity and specificity of monoclonal antibodies directed against antigenic determinants of Treponema pallidum Nichols in the diagnosis of syphilis
... Murine anti-Treponema pallidum monoclonal antibodies were employed in studies on sensitivity and specificity of binding to examine their potential for use in the detection of low numbers[r] ... See full document
7
A longitudinal evaluation of Treponema pallidumPCR testing in early syphilis
... ensure detection of both weak and strong speci- ...of detection of the ...T pallidum (Nichols) suspension which had been previously used in the T pallidum immobilization ...of detection ... See full document
6
Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
... less sensitive than the isolation of virus from serum or plasma and who saw moderate increases in the rate of isolation of virus from patients with primary infections and if the sam- ples were collected during the ... See full document
6
Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... a sensitive, specific and fast diagnosis for rabies virus, even when samples are in a decomposed ...SS- PCR and Multiplex PCR for epidemiological characteriza- tion of the virus ... See full document
6
Fast Duplex One Step Reverse Transcriptase PCR for Rapid Differential Detection of West Nile and Japanese Encephalitis Viruses
... and reverse transcriptase PCR (RT-PCR) methods have been used to develop sensitive and specific assays for the identification of WNV (13, ...(TaqMan) PCR, SYBR green-based ... See full document
5
Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... (L3) detection assay provides a more direct measure of transmission risk and may be useful as a sensitive invasive method for monitoring GPELF ...multiplex reverse transcription polymerase chain ... See full document
5
Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... novel reverse transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific G and P ... See full document
9
Simultaneous Detection of Rift Valley Fever, Bluetongue, Rinderpest, and Peste des Petits Ruminants Viruses by a Single Tube Multiplex Reverse Transcriptase PCR Assay Using a Dual Priming Oligonucleotide System
... virus-specific PCR-based assays have been devel- oped as an alternative to virus isolation for the diagnosis of individual ...acid detection techniques (e.g., PCR methods) allow rapid and ... See full document
6
A report on Japanese encephalitis in Mizoram: clinical investigation and diagnosis
... However, reverse transcriptase polymerase chain reaction (RT-PCR) based methodologies have resulted in increased sensitivity for the detection of JEV viruses in clinical samples, and offers ... See full document
5
Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR
... the detection of hRSV infection, yet both of these methods have significant ...verse transcriptase PCR (RT-PCR), DFA lacks sensitivity, is subjective, and is dependent on the quality and ... See full document
5
Single tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis
... Sputum processing. For those experiments which involved human sputum, residual, purulent sputum samples were obtained from the Harbor-UCLA Med- ical Center Microbiology Laboratory and were treated with the mucolytic ... See full document
5
Molecular Characterization of Mycoplasma pneumoniae Infections in Two Rural Populations of Thailand from 2009 to 2012
... when an atypical respiratory bacterium, such as M. pneumoniae, is suspected. However, there has been a notable spread of macrolide resistance since 2000, with prevalence now ranging from approximately 10% to 15% in the ... See full document
12
Related subjects