[PDF] Top 20 Determination of the origin-specific DNA-binding domain of polyomavirus large T antigen.
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Determination of the origin-specific DNA-binding domain of polyomavirus large T antigen.
... Fusion proteins of large T antigen lacking the first 200 N-terminal amino acids bound specifically to polyomavirus origin DNA; however, deletions beyond this site resulted in unstable pr[r] ... See full document
6
Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... for T-ag assembly events, we analyzed binding to larger subfragments of the core ...support T-ag assem- bly, albeit at lower levels compared to wild-type sequences, indicates that both ... See full document
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The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document
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J Domain-Independent Regulation of the Rb Family by Polyomavirus Large T Antigen
... LT binding to Rb may simply prevent the interaction necessary for its antiapoptotic ...Rb binding to MDM2 can affect its antiapoptotic activity ...a specific transcriptional regulator that becomes ... See full document
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The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... (SV40) T antigen is a multifunctional pro- tein and the only viral protein involved in SV40 DNA replica- ...initiate DNA replication, T antigen must interact with three distinct ... See full document
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Polyomavirus Large T Antigen Binds Symmetrical Repeats at the Viral Origin in an Asymmetrical Manner
... the origin of replication (8, 30, ...viral origin sequences, combined with the protein asymmetry imposed by steric constraints when the OBDs bind the major groove of the DNA, raises the question of ... See full document
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Elements of the polyomavirus replication origin required for homologous recombination mediated by large T antigen.
... for T-ag-mediated recombina- ...and large T-ag in D ...the origin region. The polyoma- virus origin comprises a core component and two auxiliary ...The origin core (ori-core) is ... See full document
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Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document
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Sequences flanking the pentanucleotide T-antigen binding sites in the polyomavirus core origin help determine selectivity of DNA replication.
... cis-acting origin of DNA replication, with its auxiliary DNA elements, and the trans-acting viral large tumor antigen (T ...and DNA chain ...the T antigen of ... See full document
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Functional implications of mutations within polyomavirus large T antigen Rb-binding domain: effects on pRb and p107 binding in vitro and immortalization activity in vivo.
... mutant DNA to be assessed (10 mg) was precipitated with 10 mg of pSV2Neo and left on the cells for 16 ...SV40 T (52) inserted downstream of the b -actin promoter and intervening ...SV40 T coding ... See full document
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... of DNA replication is a complicated process that requires very precise protein-DNA interactions at replication origins (1, ...(SV40) DNA has served as a useful model system for studying initiation ... See full document
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Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.
... and DNA replication has been noted (7, ...at specific residues might be necessary for ...a specific phosphor- ...with polyomavirus LT- expressing baculoviruses from Sf9 cells also argue ... See full document
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Guanine nucleotide contacts within viral DNA sequences bound by polyomavirus large T antigen.
... 505 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Essential nucleotide contacts between the polyomavirus large T antigen and its multiple specific binding regions wit[r] ... See full document
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Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... the T-Ag OBD and its interactions with ...the T-Ag OBD by nuclear magnetic resonance (NMR) meth- ods (30) and established that it is a monomer in ...the T-Ag OBD bound in a site-specific ... See full document
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... Eukaryotic DNA replication is a complex process that is initiated by the coordinated action of several factors, including the origin recognition complex, cdt1, cdc6, and the minichro- mosome maintenance ... See full document
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The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function.
... of polyomavirus T antigens to those of the DnaJ family is ...J domain structures, four helical regions are predicted for the LT N terminus with the conserved HPD motif on the loop between helices 2 ... See full document
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Activation of CREB/ATF Sites by Polyomavirus Large T Antigen
... the domain contributes to activity, since 1-420 is much more active than ...in DNA binding, and it allows association with ...SV40 large T, which can interact with TEF1 (28) as well as ... See full document
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Discrete regions of simian virus 40 large T antigen are required for nonspecific and viral origin-specific DNA binding.
... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... detect binding of full-length T antigen to fluorescent probes under the same salt ...D, T antigen was able to bind to the TBS-containing probe in a sequence-specific manner but ... See full document
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The DNA-binding properties of polyomavirus large T antigen are altered by ATP and other nucleotides.
... In the absence of ATP and with increasing quantities of Py TAg, sites A and B as well as site C, for which the data are not shown, on the early side of the origin palindrome, were protec[r] ... See full document
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